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Development of multiplex reverse-transcription digital PCR assay for co-detection of bovine leukemia virus and bovine viral diarrhea virus in cattle.

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Title: Development of multiplex reverse-transcription digital PCR assay for co-detection of bovine leukemia virus and bovine viral diarrhea virus in cattle.
Authors: Ullah S; Graduate School of Medicine and Veterinary Medicine, University of Miyazaki, Miyazaki 889-1692, Japan; Livestock and Dairy Development Department Balochistan, Quetta 87300, Pakistan. Electronic address: shakir_ullah@med.miyazaki-u.ac.jp., Notsu K; Center for Animal Disease Control, University of Miyazaki, Miyazaki 889-2192, Japan; Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA. Electronic address: kousuke_notsu@med.miyazaki-u.ac.jp., Saito A; Center for Animal Disease Control, University of Miyazaki, Miyazaki 889-2192, Japan; Department of Veterinary Science, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan. Electronic address: sakatsuki@cc.miyazaki-u.ac.jp., Okabayashi T; Center for Animal Disease Control, University of Miyazaki, Miyazaki 889-2192, Japan; Department of Veterinary Science, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan. Electronic address: okbys81@cc.miyazaki-u.ac.jp., Mekata H; Center for Animal Disease Control, University of Miyazaki, Miyazaki 889-2192, Japan. Electronic address: mekata@cc.miyazaki-u.ac.jp., Shiokawa M; School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Musashino, Tokyo 180-8602, Japan. Electronic address: shiokawa@nvlu.ac.jp., Aoki H; School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Musashino, Tokyo 180-8602, Japan. Electronic address: aokihir@nvlu.ac.jp., Sekiguchi S; Center for Animal Disease Control, University of Miyazaki, Miyazaki 889-2192, Japan; Department of Veterinary Science, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan. Electronic address: sekiguchi@cc.miyazaki-u.ac.jp.
Source: Journal of virological methods [J Virol Methods] 2026 Feb; Vol. 340, pp. 115298. Date of Electronic Publication: 2025 Nov 06.
Publication Type: Journal Article
Journal Info: Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 8005839 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0984 (Electronic) Linking ISSN: 01660934 NLM ISO Abbreviation: J Virol Methods Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Data: Development of multiplex reverse-transcription digital PCR assay for co-detection of bovine leukemia virus and bovine viral diarrhea virus in cattle.
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  Data: <searchLink fieldCode="AU" term="%22Ullah+S%22">Ullah S</searchLink>; Graduate School of Medicine and Veterinary Medicine, University of Miyazaki, Miyazaki 889-1692, Japan; Livestock and Dairy Development Department Balochistan, Quetta 87300, Pakistan. Electronic address: shakir_ullah@med.miyazaki-u.ac.jp.<br /><searchLink fieldCode="AU" term="%22Notsu+K%22">Notsu K</searchLink>; Center for Animal Disease Control, University of Miyazaki, Miyazaki 889-2192, Japan; Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA. Electronic address: kousuke_notsu@med.miyazaki-u.ac.jp.<br /><searchLink fieldCode="AU" term="%22Saito+A%22">Saito A</searchLink>; Center for Animal Disease Control, University of Miyazaki, Miyazaki 889-2192, Japan; Department of Veterinary Science, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan. Electronic address: sakatsuki@cc.miyazaki-u.ac.jp.<br /><searchLink fieldCode="AU" term="%22Okabayashi+T%22">Okabayashi T</searchLink>; Center for Animal Disease Control, University of Miyazaki, Miyazaki 889-2192, Japan; Department of Veterinary Science, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan. Electronic address: okbys81@cc.miyazaki-u.ac.jp.<br /><searchLink fieldCode="AU" term="%22Mekata+H%22">Mekata H</searchLink>; Center for Animal Disease Control, University of Miyazaki, Miyazaki 889-2192, Japan. Electronic address: mekata@cc.miyazaki-u.ac.jp.<br /><searchLink fieldCode="AU" term="%22Shiokawa+M%22">Shiokawa M</searchLink>; School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Musashino, Tokyo 180-8602, Japan. Electronic address: shiokawa@nvlu.ac.jp.<br /><searchLink fieldCode="AU" term="%22Aoki+H%22">Aoki H</searchLink>; School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Musashino, Tokyo 180-8602, Japan. Electronic address: aokihir@nvlu.ac.jp.<br /><searchLink fieldCode="AU" term="%22Sekiguchi+S%22">Sekiguchi S</searchLink>; Center for Animal Disease Control, University of Miyazaki, Miyazaki 889-2192, Japan; Department of Veterinary Science, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan. Electronic address: sekiguchi@cc.miyazaki-u.ac.jp.
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  Data: <searchLink fieldCode="JN" term="%228005839%22">Journal of virological methods</searchLink> [J Virol Methods] 2026 Feb; Vol. 340, pp. 115298. <i>Date of Electronic Publication: </i>2025 Nov 06.
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Elsevier%2FNorth-Holland+Biomedical+Press%22">Elsevier/North-Holland Biomedical Press </searchLink><i>Country of Publication: </i>Netherlands <i>NLM ID: </i>8005839 <i>Publication Model: </i>Print-Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1879-0984 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2201660934%22">01660934 </searchLink><i>NLM ISO Abbreviation: </i>J Virol Methods <i>Subsets: </i>MEDLINE
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      – Type: doi
        Value: 10.1016/j.jviromet.2025.115298
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      – Code: eng
        Text: English
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        StartPage: 115298
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      – TitleFull: Development of multiplex reverse-transcription digital PCR assay for co-detection of bovine leukemia virus and bovine viral diarrhea virus in cattle.
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            NameFull: Ullah S
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            NameFull: Notsu K
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            NameFull: Aoki H
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            – D: 01
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              Text: 2026 Feb
              Type: published
              Y: 2026
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              Value: 340
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