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Dual Phospho-CyTOF Workflows for Comparative JAK/STAT Signaling Analysis in Human Cryopreserved PBMCs and Whole Blood.

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Title: Dual Phospho-CyTOF Workflows for Comparative JAK/STAT Signaling Analysis in Human Cryopreserved PBMCs and Whole Blood.
Authors: Ramos IE; Protein and Chemistry Section (PCS), Research Technologies Branch (RTB), National Institute of Allergy and Infectious Diseases, Bethesda, MD, USA.; Center for Human Immunology, Autoimmunity, and Inflammation (CHI), National Institute of Allergy and Infectious Diseases, Bethesda, MD, USA.; Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine, Coral Gables, FL, USA., Matthiasardottir B; National Human Genome Research Institute (NHGRI), Bethesda, MD, USA.; Computational Biology, Bioinformatics, and Genomics, Biological Sciences, University of Maryland, Baltimore, MD, USA., Hawley TS; Center for Human Immunology, Autoimmunity, and Inflammation (CHI), National Institute of Allergy and Infectious Diseases, Bethesda, MD, USA.; Flow Cytometry Section, Research Technologies Branch (RTB), National Institute of Allergy and Infectious Diseases, Bethesda, MD, USA., Han KL; Center for Human Immunology, Autoimmunity, and Inflammation (CHI), National Institute of Allergy and Infectious Diseases, Bethesda, MD, USA., Toborek M; Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine, Coral Gables, FL, USA., Douagi I; Center for Human Immunology, Autoimmunity, and Inflammation (CHI), National Institute of Allergy and Infectious Diseases, Bethesda, MD, USA.; Flow Cytometry Section, Research Technologies Branch (RTB), National Institute of Allergy and Infectious Diseases, Bethesda, MD, USA., Jones GN; Center for Human Immunology, Autoimmunity, and Inflammation (CHI), National Institute of Allergy and Infectious Diseases, Bethesda, MD, USA., Cherry JM; Center for Human Immunology, Autoimmunity, and Inflammation (CHI), National Institute of Allergy and Infectious Diseases, Bethesda, MD, USA.
Source: Bio-protocol [Bio Protoc] 2025 Nov 20; Vol. 15 (22), pp. e5512. Date of Electronic Publication: 2025 Nov 20 (Print Publication: 2025).
Publication Type: Journal Article
Journal Info: Publisher: Bio-protocol LLC Country of Publication: United States NLM ID: 101635102 Publication Model: eCollection Cited Medium: Internet ISSN: 2331-8325 (Electronic) Linking ISSN: 23318325 NLM ISO Abbreviation: Bio Protoc Subsets: PubMed not MEDLINE
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  Data: Dual Phospho-CyTOF Workflows for Comparative JAK/STAT Signaling Analysis in Human Cryopreserved PBMCs and Whole Blood.
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  Data: <searchLink fieldCode="AU" term="%22Ramos+IE%22">Ramos IE</searchLink>; Protein and Chemistry Section (PCS), Research Technologies Branch (RTB), National Institute of Allergy and Infectious Diseases, Bethesda, MD, USA.; Center for Human Immunology, Autoimmunity, and Inflammation (CHI), National Institute of Allergy and Infectious Diseases, Bethesda, MD, USA.; Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine, Coral Gables, FL, USA.<br /><searchLink fieldCode="AU" term="%22Matthiasardottir+B%22">Matthiasardottir B</searchLink>; National Human Genome Research Institute (NHGRI), Bethesda, MD, USA.; Computational Biology, Bioinformatics, and Genomics, Biological Sciences, University of Maryland, Baltimore, MD, USA.<br /><searchLink fieldCode="AU" term="%22Hawley+TS%22">Hawley TS</searchLink>; Center for Human Immunology, Autoimmunity, and Inflammation (CHI), National Institute of Allergy and Infectious Diseases, Bethesda, MD, USA.; Flow Cytometry Section, Research Technologies Branch (RTB), National Institute of Allergy and Infectious Diseases, Bethesda, MD, USA.<br /><searchLink fieldCode="AU" term="%22Han+KL%22">Han KL</searchLink>; Center for Human Immunology, Autoimmunity, and Inflammation (CHI), National Institute of Allergy and Infectious Diseases, Bethesda, MD, USA.<br /><searchLink fieldCode="AU" term="%22Toborek+M%22">Toborek M</searchLink>; Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine, Coral Gables, FL, USA.<br /><searchLink fieldCode="AU" term="%22Douagi+I%22">Douagi I</searchLink>; Center for Human Immunology, Autoimmunity, and Inflammation (CHI), National Institute of Allergy and Infectious Diseases, Bethesda, MD, USA.; Flow Cytometry Section, Research Technologies Branch (RTB), National Institute of Allergy and Infectious Diseases, Bethesda, MD, USA.<br /><searchLink fieldCode="AU" term="%22Jones+GN%22">Jones GN</searchLink>; Center for Human Immunology, Autoimmunity, and Inflammation (CHI), National Institute of Allergy and Infectious Diseases, Bethesda, MD, USA.<br /><searchLink fieldCode="AU" term="%22Cherry+JM%22">Cherry JM</searchLink>; Center for Human Immunology, Autoimmunity, and Inflammation (CHI), National Institute of Allergy and Infectious Diseases, Bethesda, MD, USA.
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  Data: <searchLink fieldCode="JN" term="%22101635102%22">Bio-protocol</searchLink> [Bio Protoc] 2025 Nov 20; Vol. 15 (22), pp. e5512. <i>Date of Electronic Publication: </i>2025 Nov 20 (<i>Print Publication: </i>2025).
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              Text: 2025 Nov 20
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