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Anti-CRISPR-mediated continuous directed evolution of CRISPR-Cas9 in human cells.

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Title: Anti-CRISPR-mediated continuous directed evolution of CRISPR-Cas9 in human cells.
Authors: Sabol AL; Contributed equally to this work.; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA., Mengiste AA; Contributed equally to this work.; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA., Sreekanth V; Contributed equally to this work.; Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA., Singh P; Contributed equally to this work.; Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA., Hendel SJ; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA., Tran MTN; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA., Barybin AM; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA., Chaudhary S; Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA., Harris RM; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA., Liivak K; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA., Severance ZC; Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA., Locicero CM; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA., Kailass K; Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA., Lee C; Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA., Xu LQ; Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA., Butty VL; Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.; BioMicro Center, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA., Choudhary A; Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA.; Divisions of Renal Medicine and Engineering, Brigham and Women's Hospital, Boston, Massachusetts, 02115, USA.; Department of Medicine, Harvard Medical School, Boston, MA, 02115, USA., Shoulders MD; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.; Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA.; Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
Source: BioRxiv : the preprint server for biology [bioRxiv] 2025 Dec 11. Date of Electronic Publication: 2025 Dec 11.
Publication Type: Journal Article; Preprint
Journal Info: Country of Publication: United States NLM ID: 101680187 Publication Model: Electronic Cited Medium: Internet ISSN: 2692-8205 (Electronic) Linking ISSN: 26928205 NLM ISO Abbreviation: bioRxiv Subsets: PubMed not MEDLINE
Database: MEDLINE Ultimate
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  Data: <searchLink fieldCode="AU" term="%22Sabol+AL%22">Sabol AL</searchLink>; Contributed equally to this work.; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.<br /><searchLink fieldCode="AU" term="%22Mengiste+AA%22">Mengiste AA</searchLink>; Contributed equally to this work.; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.<br /><searchLink fieldCode="AU" term="%22Sreekanth+V%22">Sreekanth V</searchLink>; Contributed equally to this work.; Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA.<br /><searchLink fieldCode="AU" term="%22Singh+P%22">Singh P</searchLink>; Contributed equally to this work.; Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA.<br /><searchLink fieldCode="AU" term="%22Hendel+SJ%22">Hendel SJ</searchLink>; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.<br /><searchLink fieldCode="AU" term="%22Tran+MTN%22">Tran MTN</searchLink>; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.<br /><searchLink fieldCode="AU" term="%22Barybin+AM%22">Barybin AM</searchLink>; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.<br /><searchLink fieldCode="AU" term="%22Chaudhary+S%22">Chaudhary S</searchLink>; Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA.<br /><searchLink fieldCode="AU" term="%22Harris+RM%22">Harris RM</searchLink>; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.<br /><searchLink fieldCode="AU" term="%22Liivak+K%22">Liivak K</searchLink>; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.<br /><searchLink fieldCode="AU" term="%22Severance+ZC%22">Severance ZC</searchLink>; Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA.<br /><searchLink fieldCode="AU" term="%22Locicero+CM%22">Locicero CM</searchLink>; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.<br /><searchLink fieldCode="AU" term="%22Kailass+K%22">Kailass K</searchLink>; Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA.<br /><searchLink fieldCode="AU" term="%22Lee+C%22">Lee C</searchLink>; Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA.<br /><searchLink fieldCode="AU" term="%22Xu+LQ%22">Xu LQ</searchLink>; Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA.<br /><searchLink fieldCode="AU" term="%22Butty+VL%22">Butty VL</searchLink>; Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.; BioMicro Center, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.<br /><searchLink fieldCode="AU" term="%22Choudhary+A%22">Choudhary A</searchLink>; Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA.; Divisions of Renal Medicine and Engineering, Brigham and Women's Hospital, Boston, Massachusetts, 02115, USA.; Department of Medicine, Harvard Medical School, Boston, MA, 02115, USA.<br /><searchLink fieldCode="AU" term="%22Shoulders+MD%22">Shoulders MD</searchLink>; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.; Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA.; Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
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