A practical workflow for fixation and autofluorescence reduction in correlative light and electron microscopy of postmortem human brain tissue.

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Title: A practical workflow for fixation and autofluorescence reduction in correlative light and electron microscopy of postmortem human brain tissue.
Authors: Lee HE; Department of Anatomy, Catholic Neuroscience Institute, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea; Department of Medical Science, College of Medicine, Graduate School of The Catholic University of Korea, Seoul, Republic of Korea., Kim HL; Integrative Research Support Center, Laboratory of Electron Microscope, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea., Cho A; Department of Anatomy, Catholic Neuroscience Institute, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea; Biophysic Group, Department of Physics, Friedrich-Alexander Universität Erlangen-Nürberg (FAU) Max-Planck-Zentrum für Physik und Medizin, Erlangen, Germany., Kim DG; Department of Anatomy, Catholic Neuroscience Institute, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea; Department of Medical Science, College of Medicine, Graduate School of The Catholic University of Korea, Seoul, Republic of Korea., Park MJ; Department of Anatomy, Catholic Neuroscience Institute, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea; Department of Medical Science, College of Medicine, Graduate School of The Catholic University of Korea, Seoul, Republic of Korea., Oh Y; Integrative Research Support Center, Laboratory of Electron Microscope, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea., Park YS; Department of Anatomy, Catholic Neuroscience Institute, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea; Department of Medical Science, College of Medicine, Graduate School of The Catholic University of Korea, Seoul, Republic of Korea., Han RT; Biomedical Research Division, Korea Institute of Science and Technology, Seoul, Republic of Korea; KHU-KIST Department of Converging Science and Technology, Kyung Hee University, Seoul, Republic of Korea., Lee MY; Department of Anatomy, Catholic Neuroscience Institute, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea; Department of Medical Science, College of Medicine, Graduate School of The Catholic University of Korea, Seoul, Republic of Korea., Riew TR; Department of Anatomy, Catholic Neuroscience Institute, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea; Department of Medical Science, College of Medicine, Graduate School of The Catholic University of Korea, Seoul, Republic of Korea. Electronic address: taeryong.riew@catholic.ac.kr.
Source: Tissue & cell [Tissue Cell] 2026 Apr; Vol. 99, pp. 103284. Date of Electronic Publication: 2025 Dec 16.
Publication Type: Journal Article
Journal Info: Publisher: Churchill Livingstone Country of Publication: Scotland NLM ID: 0214745 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-3072 (Electronic) Linking ISSN: 00408166 NLM ISO Abbreviation: Tissue Cell Subsets: MEDLINE
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  Data: A practical workflow for fixation and autofluorescence reduction in correlative light and electron microscopy of postmortem human brain tissue.
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  Data: <searchLink fieldCode="AU" term="%22Lee+HE%22">Lee HE</searchLink>; Department of Anatomy, Catholic Neuroscience Institute, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea; Department of Medical Science, College of Medicine, Graduate School of The Catholic University of Korea, Seoul, Republic of Korea.<br /><searchLink fieldCode="AU" term="%22Kim+HL%22">Kim HL</searchLink>; Integrative Research Support Center, Laboratory of Electron Microscope, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.<br /><searchLink fieldCode="AU" term="%22Cho+A%22">Cho A</searchLink>; Department of Anatomy, Catholic Neuroscience Institute, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea; Biophysic Group, Department of Physics, Friedrich-Alexander Universität Erlangen-Nürberg (FAU) Max-Planck-Zentrum für Physik und Medizin, Erlangen, Germany.<br /><searchLink fieldCode="AU" term="%22Kim+DG%22">Kim DG</searchLink>; Department of Anatomy, Catholic Neuroscience Institute, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea; Department of Medical Science, College of Medicine, Graduate School of The Catholic University of Korea, Seoul, Republic of Korea.<br /><searchLink fieldCode="AU" term="%22Park+MJ%22">Park MJ</searchLink>; Department of Anatomy, Catholic Neuroscience Institute, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea; Department of Medical Science, College of Medicine, Graduate School of The Catholic University of Korea, Seoul, Republic of Korea.<br /><searchLink fieldCode="AU" term="%22Oh+Y%22">Oh Y</searchLink>; Integrative Research Support Center, Laboratory of Electron Microscope, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.<br /><searchLink fieldCode="AU" term="%22Park+YS%22">Park YS</searchLink>; Department of Anatomy, Catholic Neuroscience Institute, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea; Department of Medical Science, College of Medicine, Graduate School of The Catholic University of Korea, Seoul, Republic of Korea.<br /><searchLink fieldCode="AU" term="%22Han+RT%22">Han RT</searchLink>; Biomedical Research Division, Korea Institute of Science and Technology, Seoul, Republic of Korea; KHU-KIST Department of Converging Science and Technology, Kyung Hee University, Seoul, Republic of Korea.<br /><searchLink fieldCode="AU" term="%22Lee+MY%22">Lee MY</searchLink>; Department of Anatomy, Catholic Neuroscience Institute, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea; Department of Medical Science, College of Medicine, Graduate School of The Catholic University of Korea, Seoul, Republic of Korea.<br /><searchLink fieldCode="AU" term="%22Riew+TR%22">Riew TR</searchLink>; Department of Anatomy, Catholic Neuroscience Institute, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea; Department of Medical Science, College of Medicine, Graduate School of The Catholic University of Korea, Seoul, Republic of Korea. Electronic address: taeryong.riew@catholic.ac.kr.
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  Data: <searchLink fieldCode="JN" term="%220214745%22">Tissue & cell</searchLink> [Tissue Cell] 2026 Apr; Vol. 99, pp. 103284. <i>Date of Electronic Publication: </i>2025 Dec 16.
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Churchill+Livingstone%22">Churchill Livingstone </searchLink><i>Country of Publication: </i>Scotland <i>NLM ID: </i>0214745 <i>Publication Model: </i>Print-Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1532-3072 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2200408166%22">00408166 </searchLink><i>NLM ISO Abbreviation: </i>Tissue Cell <i>Subsets: </i>MEDLINE
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              Text: 2026 Apr
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