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Integrated fluorescence light microscopy-guided cryo-focused ion beam-milling for in situ montage cryo-ET.

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Title: Integrated fluorescence light microscopy-guided cryo-focused ion beam-milling for in situ montage cryo-ET.
Authors: Yang JE; Department of Biochemistry, University of Wisconsin, Madison, WI, USA.; Cryo-Electron Microscopy Research Center, Department of Biochemistry, University of Wisconsin, Madison, WI, USA.; Midwest Center for Cryo-Electron Tomography, Department of Biochemistry, University of Wisconsin, Madison, WI, USA., Vrbovská V; Thermo Fisher Scientific Brno, Brno, Czech Republic., Mitchell JM; Department of Biomolecular Chemistry, University of Wisconsin, Madison, WI, USA.; Department of Medicine, University of Wisconsin, Madison, WI, USA.; Investigative Medicine, Homer Stryker M.D. School of Medicine, Western Michigan University, Kalamazoo, MI, USA., Franke T; Thermo Fisher Scientific Planegg, Bavaria, Germany., Sibert BS; Department of Biochemistry, University of Wisconsin, Madison, WI, USA.; Cryo-Electron Microscopy Research Center, Department of Biochemistry, University of Wisconsin, Madison, WI, USA.; Midwest Center for Cryo-Electron Tomography, Department of Biochemistry, University of Wisconsin, Madison, WI, USA., Larson MR; Department of Biochemistry, University of Wisconsin, Madison, WI, USA.; Cryo-Electron Microscopy Research Center, Department of Biochemistry, University of Wisconsin, Madison, WI, USA.; Midwest Center for Cryo-Electron Tomography, Department of Biochemistry, University of Wisconsin, Madison, WI, USA., Hall AS; Thermo Fisher Scientific, Houston, TX, USA., Rigort A; Thermo Fisher Scientific Planegg, Bavaria, Germany., Mosher DF; Department of Biomolecular Chemistry, University of Wisconsin, Madison, WI, USA.; Thermo Fisher Scientific, Houston, TX, USA.; Investigative Medicine, Homer Stryker M.D. School of Medicine, Western Michigan University, Kalamazoo, MI, USA., Mitchels J; Thermo Fisher Scientific Brno, Brno, Czech Republic., Wright ER; Department of Biochemistry, University of Wisconsin, Madison, WI, USA. erwright2@wisc.edu.; Cryo-Electron Microscopy Research Center, Department of Biochemistry, University of Wisconsin, Madison, WI, USA. erwright2@wisc.edu.; Midwest Center for Cryo-Electron Tomography, Department of Biochemistry, University of Wisconsin, Madison, WI, USA. erwright2@wisc.edu.; Morgridge Institute for Research, Madison, WI, USA. erwright2@wisc.edu.; DOE Great Lakes Bioenergy Research Center, University of Wisconsin, Madison, WI, USA. erwright2@wisc.edu.
Source: Nature protocols [Nat Protoc] 2026 Jun; Vol. 21 (6), pp. 2552-2587. Date of Electronic Publication: 2026 Jan 30.
Publication Type: Journal Article; Review
Journal Info: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101284307 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1750-2799 (Electronic) Linking ISSN: 17502799 NLM ISO Abbreviation: Nat Protoc Subsets: MEDLINE
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  Data: <searchLink fieldCode="AU" term="%22Yang+JE%22">Yang JE</searchLink>; Department of Biochemistry, University of Wisconsin, Madison, WI, USA.; Cryo-Electron Microscopy Research Center, Department of Biochemistry, University of Wisconsin, Madison, WI, USA.; Midwest Center for Cryo-Electron Tomography, Department of Biochemistry, University of Wisconsin, Madison, WI, USA.<br /><searchLink fieldCode="AU" term="%22Vrbovská+V%22">Vrbovská V</searchLink>; Thermo Fisher Scientific Brno, Brno, Czech Republic.<br /><searchLink fieldCode="AU" term="%22Mitchell+JM%22">Mitchell JM</searchLink>; Department of Biomolecular Chemistry, University of Wisconsin, Madison, WI, USA.; Department of Medicine, University of Wisconsin, Madison, WI, USA.; Investigative Medicine, Homer Stryker M.D. School of Medicine, Western Michigan University, Kalamazoo, MI, USA.<br /><searchLink fieldCode="AU" term="%22Franke+T%22">Franke T</searchLink>; Thermo Fisher Scientific Planegg, Bavaria, Germany.<br /><searchLink fieldCode="AU" term="%22Sibert+BS%22">Sibert BS</searchLink>; Department of Biochemistry, University of Wisconsin, Madison, WI, USA.; Cryo-Electron Microscopy Research Center, Department of Biochemistry, University of Wisconsin, Madison, WI, USA.; Midwest Center for Cryo-Electron Tomography, Department of Biochemistry, University of Wisconsin, Madison, WI, USA.<br /><searchLink fieldCode="AU" term="%22Larson+MR%22">Larson MR</searchLink>; Department of Biochemistry, University of Wisconsin, Madison, WI, USA.; Cryo-Electron Microscopy Research Center, Department of Biochemistry, University of Wisconsin, Madison, WI, USA.; Midwest Center for Cryo-Electron Tomography, Department of Biochemistry, University of Wisconsin, Madison, WI, USA.<br /><searchLink fieldCode="AU" term="%22Hall+AS%22">Hall AS</searchLink>; Thermo Fisher Scientific, Houston, TX, USA.<br /><searchLink fieldCode="AU" term="%22Rigort+A%22">Rigort A</searchLink>; Thermo Fisher Scientific Planegg, Bavaria, Germany.<br /><searchLink fieldCode="AU" term="%22Mosher+DF%22">Mosher DF</searchLink>; Department of Biomolecular Chemistry, University of Wisconsin, Madison, WI, USA.; Thermo Fisher Scientific, Houston, TX, USA.; Investigative Medicine, Homer Stryker M.D. School of Medicine, Western Michigan University, Kalamazoo, MI, USA.<br /><searchLink fieldCode="AU" term="%22Mitchels+J%22">Mitchels J</searchLink>; Thermo Fisher Scientific Brno, Brno, Czech Republic.<br /><searchLink fieldCode="AU" term="%22Wright+ER%22">Wright ER</searchLink>; Department of Biochemistry, University of Wisconsin, Madison, WI, USA. erwright2@wisc.edu.; Cryo-Electron Microscopy Research Center, Department of Biochemistry, University of Wisconsin, Madison, WI, USA. erwright2@wisc.edu.; Midwest Center for Cryo-Electron Tomography, Department of Biochemistry, University of Wisconsin, Madison, WI, USA. erwright2@wisc.edu.; Morgridge Institute for Research, Madison, WI, USA. erwright2@wisc.edu.; DOE Great Lakes Bioenergy Research Center, University of Wisconsin, Madison, WI, USA. erwright2@wisc.edu.
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  Data: <searchLink fieldCode="JN" term="%22101284307%22">Nature protocols</searchLink> [Nat Protoc] 2026 Jun; Vol. 21 (6), pp. 2552-2587. <i>Date of Electronic Publication: </i>2026 Jan 30.
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