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Sandwich ELISA using newly generated monoclonal antibodies quantifies circulating neuropeptide F in Drosophila melanogaster.

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Title: Sandwich ELISA using newly generated monoclonal antibodies quantifies circulating neuropeptide F in Drosophila melanogaster.
Authors: Higashida M; Graduate School of Science and Technology, University of Tsukuba, Tsukuba, Ibaraki, 305-8572, Japan., Kobayashi T; Division of Molecular Genetics, Shigei Medical Research Institute, Okayama, 701-0202, Japan., Hoshino R; Graduate School of Science and Technology, University of Tsukuba, Tsukuba, Ibaraki, 305-8572, Japan; Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, Tsukuba, Ibaraki, 305-8577, Japan., Yoshinari Y; Graduate School of Science and Technology, University of Tsukuba, Tsukuba, Ibaraki, 305-8572, Japan; Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, Tsukuba, Ibaraki, 305-8577, Japan; Metabolic Regulation and Genetics, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Gunma, 371-8512, Japan., Chen J; Ecology, Evolution, & Organismal Biology, Center on the Biology of Aging, Brown University, Providence, RI, 02912, United States of America., Tatar M; Ecology, Evolution, & Organismal Biology, Center on the Biology of Aging, Brown University, Providence, RI, 02912, United States of America., Okamoto N; Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, Tsukuba, Ibaraki, 305-8577, Japan., Matsuyama M; Division of Molecular Genetics, Shigei Medical Research Institute, Okayama, 701-0202, Japan. Electronic address: matsuyama@shigei.or.jp., Niwa R; Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, Tsukuba, Ibaraki, 305-8577, Japan. Electronic address: ryusuke-niwa@tara.tsukuba.ac.jp.
Source: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2026 May 28; Vol. 815, pp. 153680. Date of Electronic Publication: 2026 Mar 25.
Publication Type: Journal Article
Journal Info: Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2104 (Electronic) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE
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  Data: Sandwich ELISA using newly generated monoclonal antibodies quantifies circulating neuropeptide F in Drosophila melanogaster.
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  Data: <searchLink fieldCode="AU" term="%22Higashida+M%22">Higashida M</searchLink>; Graduate School of Science and Technology, University of Tsukuba, Tsukuba, Ibaraki, 305-8572, Japan.<br /><searchLink fieldCode="AU" term="%22Kobayashi+T%22">Kobayashi T</searchLink>; Division of Molecular Genetics, Shigei Medical Research Institute, Okayama, 701-0202, Japan.<br /><searchLink fieldCode="AU" term="%22Hoshino+R%22">Hoshino R</searchLink>; Graduate School of Science and Technology, University of Tsukuba, Tsukuba, Ibaraki, 305-8572, Japan; Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, Tsukuba, Ibaraki, 305-8577, Japan.<br /><searchLink fieldCode="AU" term="%22Yoshinari+Y%22">Yoshinari Y</searchLink>; Graduate School of Science and Technology, University of Tsukuba, Tsukuba, Ibaraki, 305-8572, Japan; Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, Tsukuba, Ibaraki, 305-8577, Japan; Metabolic Regulation and Genetics, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Gunma, 371-8512, Japan.<br /><searchLink fieldCode="AU" term="%22Chen+J%22">Chen J</searchLink>; Ecology, Evolution, & Organismal Biology, Center on the Biology of Aging, Brown University, Providence, RI, 02912, United States of America.<br /><searchLink fieldCode="AU" term="%22Tatar+M%22">Tatar M</searchLink>; Ecology, Evolution, & Organismal Biology, Center on the Biology of Aging, Brown University, Providence, RI, 02912, United States of America.<br /><searchLink fieldCode="AU" term="%22Okamoto+N%22">Okamoto N</searchLink>; Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, Tsukuba, Ibaraki, 305-8577, Japan.<br /><searchLink fieldCode="AU" term="%22Matsuyama+M%22">Matsuyama M</searchLink>; Division of Molecular Genetics, Shigei Medical Research Institute, Okayama, 701-0202, Japan. Electronic address: matsuyama@shigei.or.jp.<br /><searchLink fieldCode="AU" term="%22Niwa+R%22">Niwa R</searchLink>; Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, Tsukuba, Ibaraki, 305-8577, Japan. Electronic address: ryusuke-niwa@tara.tsukuba.ac.jp.
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  Data: <searchLink fieldCode="JN" term="%220372516%22">Biochemical and biophysical research communications</searchLink> [Biochem Biophys Res Commun] 2026 May 28; Vol. 815, pp. 153680. <i>Date of Electronic Publication: </i>2026 Mar 25.
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Elsevier%22">Elsevier </searchLink><i>Country of Publication: </i>United States <i>NLM ID: </i>0372516 <i>Publication Model: </i>Print-Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1090-2104 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%220006291X%22">0006291X </searchLink><i>NLM ISO Abbreviation: </i>Biochem Biophys Res Commun <i>Subsets: </i>MEDLINE
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              Text: 2026 May 28
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