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Methodological guidelines for P2X receptor assays and data interpretation.

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Title: Methodological guidelines for P2X receptor assays and data interpretation.
Authors: Gorecki DC; School of Medicine, Pharmacy and Biomedical Sciences, University of Portsmouth, Portsmouth, UK. darek.gorecki@port.ac.uk., Adinolfi E; Department of Medical Sciences, University of Ferrara, Ferrara, Italy., Adriouch S; Univ Rouen Normandie, INSERM, U1234, Pathophysiology Autoimmunity and Immunotherapy (PANTHER), Normandie Univ, Rouen, France., Coutinho-Silva R; Laboratório de Imunofisiologia, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., Engel T; Department of Physiology & Medical Physics, RCSI University of Medicine and Health Sciences, Dublin, Ireland.; FutureNeuro Research Ireland Centre, RCSI University of Medicine and Health Sciences, Dublin, Ireland., Gölöncsér F; Laboratory of Molecular Pharmacology, HUN-REN Institute of Experimental Medicine, Budapest, Hungary., Haag F; Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Illes P; Rudolf Boehm Institute for Pharmacology and Toxicology, University of Leipzig, Leipzig, Germany.; Molecular Horizons and School of Science, University of Wollongong, Wollongong, NSW, Australia., Jacobson KA; Molecular Recognition Section, Laboratory of Bioorganic Chemistry, NIDDK, National Institutes of Health, Bethesda, MD, USA., Koch-Nolte F; Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Mansoor SE; Department of Chemical Physiology & Biochemistry; Division of Cardiovascular Medicine, Knight Cardiovascular Institute, Oregon Health & Science University, Portland, OR, USA., Matute C; Laboratory of Neurobiology, Universidad del País Vasco (UPV/EHU), CIBERNED-Instituto Carlos III, IIS-Biobizkaia, Department of Neurosciences, School of Medicine and Nursing, Leioa, Spain., Melino G; University of Rome 'Tor Vergata', Rome, Italy., Novak I; Department of Biology, Universitetsparken 13, University of Copenhagen, Copenhagen Ø, Denmark., Pegoraro A; Department of Medical Sciences, University of Ferrara, Ferrara, Italy., Pelegrin P; Department of Biochemistry and Molecular Biology and Immunology, Faculty of Medicine, University of Murcia, BioMedical Research Institute of Murcia, Murcia, Spain., Piacentini M; University of Rome 'Tor Vergata', Rome, Italy., Robson SC; Departments of Anesthesia and Medicine, CLS 612, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA., Rumney RMH; Bone and Joint Group, School of Human Development and Health, Faculty of Medicine, University Hospital Southampton, University of Southampton, Southampton, England., Seman M; Univ Rouen Normandie, INSERM, U1234, Pathophysiology Autoimmunity and Immunotherapy (PANTHER), Normandie Univ, Rouen, France., Sluyter R; Molecular Horizons and School of Science, University of Wollongong, Wollongong, NSW, Australia., Sperlagh B; Laboratory of Molecular Pharmacology, HUN-REN Institute of Experimental Medicine, Budapest, Hungary., Tang Y; International Joint Research Centre on Purinergic Signalling, School of Health and Rehabilitation, Chengdu University of Traditional Chinese Medicine, Chengdu, China., Tarantini M; Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Ulrich H; Departamento de Bioquimica, Instituto de Quimica Universidade de Sao Paulo, Sao Paulo, Brazil., Vouret-Craviari V; Université Côte d'Azur, CNRS, UMR7284, INSERM, U1081, IRCAN, Nice, France & IHU RespirERA, Nice, France., Ye Q; International Joint Research Centre on Purinergic Signalling, School of Health and Rehabilitation, Chengdu University of Traditional Chinese Medicine, Chengdu, China., Yegutkin GG; MediCity Research Laboratory and InFLAMES Flagship, University of Turku, Turku, Finland., Verkhratsky A; Faculty of Biology, Medicine, and Health, The University of Manchester, Manchester, UK.
Source: Cell death & disease [Cell Death Dis] 2026 Apr 20; Vol. 17 (1). Date of Electronic Publication: 2026 Apr 20.
Publication Type: Journal Article; Review
Journal Info: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101524092 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-4889 (Electronic) NLM ISO Abbreviation: Cell Death Dis Subsets: MEDLINE
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  Data: <searchLink fieldCode="AU" term="%22Gorecki+DC%22">Gorecki DC</searchLink>; School of Medicine, Pharmacy and Biomedical Sciences, University of Portsmouth, Portsmouth, UK. darek.gorecki@port.ac.uk.<br /><searchLink fieldCode="AU" term="%22Adinolfi+E%22">Adinolfi E</searchLink>; Department of Medical Sciences, University of Ferrara, Ferrara, Italy.<br /><searchLink fieldCode="AU" term="%22Adriouch+S%22">Adriouch S</searchLink>; Univ Rouen Normandie, INSERM, U1234, Pathophysiology Autoimmunity and Immunotherapy (PANTHER), Normandie Univ, Rouen, France.<br /><searchLink fieldCode="AU" term="%22Coutinho-Silva+R%22">Coutinho-Silva R</searchLink>; Laboratório de Imunofisiologia, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.<br /><searchLink fieldCode="AU" term="%22Engel+T%22">Engel T</searchLink>; Department of Physiology & Medical Physics, RCSI University of Medicine and Health Sciences, Dublin, Ireland.; FutureNeuro Research Ireland Centre, RCSI University of Medicine and Health Sciences, Dublin, Ireland.<br /><searchLink fieldCode="AU" term="%22Gölöncsér+F%22">Gölöncsér F</searchLink>; Laboratory of Molecular Pharmacology, HUN-REN Institute of Experimental Medicine, Budapest, Hungary.<br /><searchLink fieldCode="AU" term="%22Haag+F%22">Haag F</searchLink>; Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.<br /><searchLink fieldCode="AU" term="%22Illes+P%22">Illes P</searchLink>; Rudolf Boehm Institute for Pharmacology and Toxicology, University of Leipzig, Leipzig, Germany.; Molecular Horizons and School of Science, University of Wollongong, Wollongong, NSW, Australia.<br /><searchLink fieldCode="AU" term="%22Jacobson+KA%22">Jacobson KA</searchLink>; Molecular Recognition Section, Laboratory of Bioorganic Chemistry, NIDDK, National Institutes of Health, Bethesda, MD, USA.<br /><searchLink fieldCode="AU" term="%22Koch-Nolte+F%22">Koch-Nolte F</searchLink>; Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.<br /><searchLink fieldCode="AU" term="%22Mansoor+SE%22">Mansoor SE</searchLink>; Department of Chemical Physiology & Biochemistry; Division of Cardiovascular Medicine, Knight Cardiovascular Institute, Oregon Health & Science University, Portland, OR, USA.<br /><searchLink fieldCode="AU" term="%22Matute+C%22">Matute C</searchLink>; Laboratory of Neurobiology, Universidad del País Vasco (UPV/EHU), CIBERNED-Instituto Carlos III, IIS-Biobizkaia, Department of Neurosciences, School of Medicine and Nursing, Leioa, Spain.<br /><searchLink fieldCode="AU" term="%22Melino+G%22">Melino G</searchLink>; University of Rome 'Tor Vergata', Rome, Italy.<br /><searchLink fieldCode="AU" term="%22Novak+I%22">Novak I</searchLink>; Department of Biology, Universitetsparken 13, University of Copenhagen, Copenhagen Ø, Denmark.<br /><searchLink fieldCode="AU" term="%22Pegoraro+A%22">Pegoraro A</searchLink>; Department of Medical Sciences, University of Ferrara, Ferrara, Italy.<br /><searchLink fieldCode="AU" term="%22Pelegrin+P%22">Pelegrin P</searchLink>; Department of Biochemistry and Molecular Biology and Immunology, Faculty of Medicine, University of Murcia, BioMedical Research Institute of Murcia, Murcia, Spain.<br /><searchLink fieldCode="AU" term="%22Piacentini+M%22">Piacentini M</searchLink>; University of Rome 'Tor Vergata', Rome, Italy.<br /><searchLink fieldCode="AU" term="%22Robson+SC%22">Robson SC</searchLink>; Departments of Anesthesia and Medicine, CLS 612, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.<br /><searchLink fieldCode="AU" term="%22Rumney+RMH%22">Rumney RMH</searchLink>; Bone and Joint Group, School of Human Development and Health, Faculty of Medicine, University Hospital Southampton, University of Southampton, Southampton, England.<br /><searchLink fieldCode="AU" term="%22Seman+M%22">Seman M</searchLink>; Univ Rouen Normandie, INSERM, U1234, Pathophysiology Autoimmunity and Immunotherapy (PANTHER), Normandie Univ, Rouen, France.<br /><searchLink fieldCode="AU" term="%22Sluyter+R%22">Sluyter R</searchLink>; Molecular Horizons and School of Science, University of Wollongong, Wollongong, NSW, Australia.<br /><searchLink fieldCode="AU" term="%22Sperlagh+B%22">Sperlagh B</searchLink>; Laboratory of Molecular Pharmacology, HUN-REN Institute of Experimental Medicine, Budapest, Hungary.<br /><searchLink fieldCode="AU" term="%22Tang+Y%22">Tang Y</searchLink>; International Joint Research Centre on Purinergic Signalling, School of Health and Rehabilitation, Chengdu University of Traditional Chinese Medicine, Chengdu, China.<br /><searchLink fieldCode="AU" term="%22Tarantini+M%22">Tarantini M</searchLink>; Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.<br /><searchLink fieldCode="AU" term="%22Ulrich+H%22">Ulrich H</searchLink>; Departamento de Bioquimica, Instituto de Quimica Universidade de Sao Paulo, Sao Paulo, Brazil.<br /><searchLink fieldCode="AU" term="%22Vouret-Craviari+V%22">Vouret-Craviari V</searchLink>; Université Côte d'Azur, CNRS, UMR7284, INSERM, U1081, IRCAN, Nice, France & IHU RespirERA, Nice, France.<br /><searchLink fieldCode="AU" term="%22Ye+Q%22">Ye Q</searchLink>; International Joint Research Centre on Purinergic Signalling, School of Health and Rehabilitation, Chengdu University of Traditional Chinese Medicine, Chengdu, China.<br /><searchLink fieldCode="AU" term="%22Yegutkin+GG%22">Yegutkin GG</searchLink>; MediCity Research Laboratory and InFLAMES Flagship, University of Turku, Turku, Finland.<br /><searchLink fieldCode="AU" term="%22Verkhratsky+A%22">Verkhratsky A</searchLink>; Faculty of Biology, Medicine, and Health, The University of Manchester, Manchester, UK.
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