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Genome editing of phylogenetically distinct bacteria using cross-species retron-mediated recombineering.

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Title: Genome editing of phylogenetically distinct bacteria using cross-species retron-mediated recombineering.
Authors: González-Delgado A; Gladstone Institute of Data Science and Biotechnology, San Francisco, CA, USA. alejandro.gonzalez@gladstone.ucsf.edu., Bonillo-Lopez L; Unitat Mixta d'Investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Autònoma de Barcelona (UAB), Bellaterra, Spain.; IRTA, Programa de Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Autònoma de Barcelona (UAB), Bellaterra, Spain.; WOAH Collaborating Centre for the Research and Control of Emerging and Re-Emerging Swine Diseases in Europe (IRTA-CReSA), Bellaterra, Spain., Johnson MS; Department of Integrative Biology, University of California Berkeley, Berkeley, CA, USA.; Environmental Genomics and Systems Biology Division, E.O. Lawrence Berkeley National Laboratory, Berkeley, CA, USA., Knödlseder N; Department of Medicine and Life Sciences, Universitat Pompeu Fabra, Barcelona, Spain.; Department of Biomedicine, Aarhus University, Aarhus, Denmark., Ko CC; Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA, USA., Lekbach Y; McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, USA., Oh JH; Department of Food Science, 1605 Linden Dr, University of Wisconsin-Madison, Madison, WI, USA., Selvakumar H; Environmental Genomics and Systems Biology Division, E.O. Lawrence Berkeley National Laboratory, Berkeley, CA, USA., Wold MC; BioTechnology Institute, University of Minnesota-Twin Cities, St. Paul, MN, USA.; Department of Plant and Microbial Biology, University of Minnesota-Twin Cities, St. Paul, MN, USA., Yu Z; McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, USA., Aragón V; Unitat Mixta d'Investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Autònoma de Barcelona (UAB), Bellaterra, Spain.; IRTA, Programa de Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Autònoma de Barcelona (UAB), Bellaterra, Spain.; WOAH Collaborating Centre for the Research and Control of Emerging and Re-Emerging Swine Diseases in Europe (IRTA-CReSA), Bellaterra, Spain., Gralnick JA; BioTechnology Institute, University of Minnesota-Twin Cities, St. Paul, MN, USA.; Department of Plant and Microbial Biology, University of Minnesota-Twin Cities, St. Paul, MN, USA., Güell M; Department of Medicine and Life Sciences, Universitat Pompeu Fabra, Barcelona, Spain.; ICREA, Institució Catalana de Recerca i Estudis Avançats, Barcelona, Spain., Hatfull GF; Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA, USA., Keitz BK; McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, USA., Koskella B; Department of Integrative Biology, University of California Berkeley, Berkeley, CA, USA., Mutalik VK; Environmental Genomics and Systems Biology Division, E.O. Lawrence Berkeley National Laboratory, Berkeley, CA, USA.; Biological systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA., van Pijkeren JP; Department of Food Science, 1605 Linden Dr, University of Wisconsin-Madison, Madison, WI, USA., Shipman SL; Gladstone Institute of Data Science and Biotechnology, San Francisco, CA, USA. seth.shipman@gladstone.ucsf.edu.; Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, CA, USA. seth.shipman@gladstone.ucsf.edu.; Biohub, San Francisco, CA, USA. seth.shipman@gladstone.ucsf.edu.
Source: Nature biotechnology [Nat Biotechnol] 2026 Apr 23. Date of Electronic Publication: 2026 Apr 23.
Publication Type: Journal Article
Journal Info: Publisher: Nature America Publishing Country of Publication: United States NLM ID: 9604648 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1546-1696 (Electronic) Linking ISSN: 10870156 NLM ISO Abbreviation: Nat Biotechnol Subsets: MEDLINE
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  Data: Genome editing of phylogenetically distinct bacteria using cross-species retron-mediated recombineering.
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  Data: <searchLink fieldCode="AU" term="%22González-Delgado+A%22">González-Delgado A</searchLink>; Gladstone Institute of Data Science and Biotechnology, San Francisco, CA, USA. alejandro.gonzalez@gladstone.ucsf.edu.<br /><searchLink fieldCode="AU" term="%22Bonillo-Lopez+L%22">Bonillo-Lopez L</searchLink>; Unitat Mixta d'Investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Autònoma de Barcelona (UAB), Bellaterra, Spain.; IRTA, Programa de Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Autònoma de Barcelona (UAB), Bellaterra, Spain.; WOAH Collaborating Centre for the Research and Control of Emerging and Re-Emerging Swine Diseases in Europe (IRTA-CReSA), Bellaterra, Spain.<br /><searchLink fieldCode="AU" term="%22Johnson+MS%22">Johnson MS</searchLink>; Department of Integrative Biology, University of California Berkeley, Berkeley, CA, USA.; Environmental Genomics and Systems Biology Division, E.O. Lawrence Berkeley National Laboratory, Berkeley, CA, USA.<br /><searchLink fieldCode="AU" term="%22Knödlseder+N%22">Knödlseder N</searchLink>; Department of Medicine and Life Sciences, Universitat Pompeu Fabra, Barcelona, Spain.; Department of Biomedicine, Aarhus University, Aarhus, Denmark.<br /><searchLink fieldCode="AU" term="%22Ko+CC%22">Ko CC</searchLink>; Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA, USA.<br /><searchLink fieldCode="AU" term="%22Lekbach+Y%22">Lekbach Y</searchLink>; McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, USA.<br /><searchLink fieldCode="AU" term="%22Oh+JH%22">Oh JH</searchLink>; Department of Food Science, 1605 Linden Dr, University of Wisconsin-Madison, Madison, WI, USA.<br /><searchLink fieldCode="AU" term="%22Selvakumar+H%22">Selvakumar H</searchLink>; Environmental Genomics and Systems Biology Division, E.O. Lawrence Berkeley National Laboratory, Berkeley, CA, USA.<br /><searchLink fieldCode="AU" term="%22Wold+MC%22">Wold MC</searchLink>; BioTechnology Institute, University of Minnesota-Twin Cities, St. Paul, MN, USA.; Department of Plant and Microbial Biology, University of Minnesota-Twin Cities, St. Paul, MN, USA.<br /><searchLink fieldCode="AU" term="%22Yu+Z%22">Yu Z</searchLink>; McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, USA.<br /><searchLink fieldCode="AU" term="%22Aragón+V%22">Aragón V</searchLink>; Unitat Mixta d'Investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Autònoma de Barcelona (UAB), Bellaterra, Spain.; IRTA, Programa de Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Autònoma de Barcelona (UAB), Bellaterra, Spain.; WOAH Collaborating Centre for the Research and Control of Emerging and Re-Emerging Swine Diseases in Europe (IRTA-CReSA), Bellaterra, Spain.<br /><searchLink fieldCode="AU" term="%22Gralnick+JA%22">Gralnick JA</searchLink>; BioTechnology Institute, University of Minnesota-Twin Cities, St. Paul, MN, USA.; Department of Plant and Microbial Biology, University of Minnesota-Twin Cities, St. Paul, MN, USA.<br /><searchLink fieldCode="AU" term="%22Güell+M%22">Güell M</searchLink>; Department of Medicine and Life Sciences, Universitat Pompeu Fabra, Barcelona, Spain.; ICREA, Institució Catalana de Recerca i Estudis Avançats, Barcelona, Spain.<br /><searchLink fieldCode="AU" term="%22Hatfull+GF%22">Hatfull GF</searchLink>; Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA, USA.<br /><searchLink fieldCode="AU" term="%22Keitz+BK%22">Keitz BK</searchLink>; McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, USA.<br /><searchLink fieldCode="AU" term="%22Koskella+B%22">Koskella B</searchLink>; Department of Integrative Biology, University of California Berkeley, Berkeley, CA, USA.<br /><searchLink fieldCode="AU" term="%22Mutalik+VK%22">Mutalik VK</searchLink>; Environmental Genomics and Systems Biology Division, E.O. Lawrence Berkeley National Laboratory, Berkeley, CA, USA.; Biological systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.<br /><searchLink fieldCode="AU" term="%22van+Pijkeren+JP%22">van Pijkeren JP</searchLink>; Department of Food Science, 1605 Linden Dr, University of Wisconsin-Madison, Madison, WI, USA.<br /><searchLink fieldCode="AU" term="%22Shipman+SL%22">Shipman SL</searchLink>; Gladstone Institute of Data Science and Biotechnology, San Francisco, CA, USA. seth.shipman@gladstone.ucsf.edu.; Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, CA, USA. seth.shipman@gladstone.ucsf.edu.; Biohub, San Francisco, CA, USA. seth.shipman@gladstone.ucsf.edu.
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