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Analysis of ligand recognition by choline O-acetyltransferase reveals thiol-reactive assay interference and weak ligand affinity in solution.

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Title: Analysis of ligand recognition by choline O-acetyltransferase reveals thiol-reactive assay interference and weak ligand affinity in solution.
Authors: Forsgren N; CBRN Defence and Security, Swedish Defence Research Agency, Umeå, Sweden., Jonsson F; CBRN Defence and Security, Swedish Defence Research Agency, Umeå, Sweden., Carlsson M; CBRN Defence and Security, Swedish Defence Research Agency, Umeå, Sweden., Sander RA; CBRN Defence and Security, Swedish Defence Research Agency, Umeå, Sweden., Larsson A; CBRN Defence and Security, Swedish Defence Research Agency, Umeå, Sweden., Lindén P; CBRN Defence and Security, Swedish Defence Research Agency, Umeå, Sweden., Linusson A; CBRN Defence and Security, Swedish Defence Research Agency, Umeå, Sweden; Department of Chemistry, Umeå University, Umeå, Sweden., Engdahl CS; CBRN Defence and Security, Swedish Defence Research Agency, Umeå, Sweden., Wiktelius D; CBRN Defence and Security, Swedish Defence Research Agency, Umeå, Sweden., Ekström F; CBRN Defence and Security, Swedish Defence Research Agency, Umeå, Sweden. Electronic address: fredrik.ekstrom@foi.se.
Source: The Journal of biological chemistry [J Biol Chem] 2026 May 07; Vol. 302 (6), pp. 113115. Date of Electronic Publication: 2026 May 07.
Publication Type: Journal Article
Journal Info: Publisher: Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Country of Publication: United States NLM ID: 2985121R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1083-351X (Electronic) Linking ISSN: 00219258 NLM ISO Abbreviation: J Biol Chem Subsets: MEDLINE
Database: MEDLINE Ultimate
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An: 42103233
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PubType: Academic Journal
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  Data: Analysis of ligand recognition by choline O-acetyltransferase reveals thiol-reactive assay interference and weak ligand affinity in solution.
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  Data: <searchLink fieldCode="AU" term="%22Forsgren+N%22">Forsgren N</searchLink>; CBRN Defence and Security, Swedish Defence Research Agency, Umeå, Sweden.<br /><searchLink fieldCode="AU" term="%22Jonsson+F%22">Jonsson F</searchLink>; CBRN Defence and Security, Swedish Defence Research Agency, Umeå, Sweden.<br /><searchLink fieldCode="AU" term="%22Carlsson+M%22">Carlsson M</searchLink>; CBRN Defence and Security, Swedish Defence Research Agency, Umeå, Sweden.<br /><searchLink fieldCode="AU" term="%22Sander+RA%22">Sander RA</searchLink>; CBRN Defence and Security, Swedish Defence Research Agency, Umeå, Sweden.<br /><searchLink fieldCode="AU" term="%22Larsson+A%22">Larsson A</searchLink>; CBRN Defence and Security, Swedish Defence Research Agency, Umeå, Sweden.<br /><searchLink fieldCode="AU" term="%22Lindén+P%22">Lindén P</searchLink>; CBRN Defence and Security, Swedish Defence Research Agency, Umeå, Sweden.<br /><searchLink fieldCode="AU" term="%22Linusson+A%22">Linusson A</searchLink>; CBRN Defence and Security, Swedish Defence Research Agency, Umeå, Sweden; Department of Chemistry, Umeå University, Umeå, Sweden.<br /><searchLink fieldCode="AU" term="%22Engdahl+CS%22">Engdahl CS</searchLink>; CBRN Defence and Security, Swedish Defence Research Agency, Umeå, Sweden.<br /><searchLink fieldCode="AU" term="%22Wiktelius+D%22">Wiktelius D</searchLink>; CBRN Defence and Security, Swedish Defence Research Agency, Umeå, Sweden.<br /><searchLink fieldCode="AU" term="%22Ekström+F%22">Ekström F</searchLink>; CBRN Defence and Security, Swedish Defence Research Agency, Umeå, Sweden. Electronic address: fredrik.ekstrom@foi.se.
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  Data: <searchLink fieldCode="JN" term="%222985121R%22">The Journal of biological chemistry</searchLink> [J Biol Chem] 2026 May 07; Vol. 302 (6), pp. 113115. <i>Date of Electronic Publication: </i>2026 May 07.
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Elsevier+Inc%2E+on+behalf+of+American+Society+for+Biochemistry+and+Molecular+Biology%22">Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology </searchLink><i>Country of Publication: </i>United States <i>NLM ID: </i>2985121R <i>Publication Model: </i>Print-Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1083-351X (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2200219258%22">00219258 </searchLink><i>NLM ISO Abbreviation: </i>J Biol Chem <i>Subsets: </i>MEDLINE
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        Value: 10.1016/j.jbc.2026.113115
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      – Code: eng
        Text: English
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        StartPage: 113115
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              Text: 2026 May 07
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