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Recommendations and considerations for hydroxyl radical protein footprinting-mass spectrometry.

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Title: Recommendations and considerations for hydroxyl radical protein footprinting-mass spectrometry.
Authors: Wecksler AT; Analytical Development & Quality Control, Genentech, South San Francisco, CA, USA. wecksler.aaron@gene.com., Wang L; Analytical Development & Quality Control, Genentech, South San Francisco, CA, USA., Bernstein LJ; Nonclincial Biostatistics, Genentech, South San Francisco, CA, USA., Huang RY; Biologics Discovery, Johnson & Johnson Innovative Medicine, Spring House, PA, USA., Gupta S; Molecule Biophysics and Integrated Bioimaging, Lawrence Berkeley National Laboratory, Berkeley, CA, USA., Kristensen LG; Molecule Biophysics and Integrated Bioimaging, Lawrence Berkeley National Laboratory, Berkeley, CA, USA., Ralston CY; Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, USA., Sobott F; School of Molecular and Cellular Biology and Astbury Centre, University of Leeds, Leeds, UK., Sun Y; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY, USA., Brenowitz M; Department of Biochemistry and Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY, USA., Farquhar ER; Department of Nutrition and Center for Synchrotron Biosciences, Case Western Reserve University, Cleveland, OH, USA., Chance MR; Department of Nutrition and Center for Synchrotron Biosciences, Case Western Reserve University, Cleveland, OH, USA., Kuang XC; Department of Chemistry, Washington University in St Louis, St Louis, MO, USA., Gross ML; Department of Chemistry, Washington University in St Louis, St Louis, MO, USA., Jones LM; Department of Chemistry and Biochemistry, University of California, San Diego, San Diego, CA, USA., Novak P; Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic., Misra SK; Department of BioMolecular Sciences, University of Mississippi, Oxford, MS, USA., Sharp JS; Department of BioMolecular Sciences, University of Mississippi, Oxford, MS, USA. jsharp@olemiss.edu.
Source: Nature methods [Nat Methods] 2026 Jun; Vol. 23 (6), pp. 1089-1099. Date of Electronic Publication: 2026 May 14.
Publication Type: Journal Article; Review
Journal Info: Publisher: Nature Pub. Group Country of Publication: United States NLM ID: 101215604 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1548-7105 (Electronic) Linking ISSN: 15487091 NLM ISO Abbreviation: Nat Methods Subsets: MEDLINE
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  Data: <searchLink fieldCode="AU" term="%22Wecksler+AT%22">Wecksler AT</searchLink>; Analytical Development & Quality Control, Genentech, South San Francisco, CA, USA. wecksler.aaron@gene.com.<br /><searchLink fieldCode="AU" term="%22Wang+L%22">Wang L</searchLink>; Analytical Development & Quality Control, Genentech, South San Francisco, CA, USA.<br /><searchLink fieldCode="AU" term="%22Bernstein+LJ%22">Bernstein LJ</searchLink>; Nonclincial Biostatistics, Genentech, South San Francisco, CA, USA.<br /><searchLink fieldCode="AU" term="%22Huang+RY%22">Huang RY</searchLink>; Biologics Discovery, Johnson & Johnson Innovative Medicine, Spring House, PA, USA.<br /><searchLink fieldCode="AU" term="%22Gupta+S%22">Gupta S</searchLink>; Molecule Biophysics and Integrated Bioimaging, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.<br /><searchLink fieldCode="AU" term="%22Kristensen+LG%22">Kristensen LG</searchLink>; Molecule Biophysics and Integrated Bioimaging, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.<br /><searchLink fieldCode="AU" term="%22Ralston+CY%22">Ralston CY</searchLink>; Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.<br /><searchLink fieldCode="AU" term="%22Sobott+F%22">Sobott F</searchLink>; School of Molecular and Cellular Biology and Astbury Centre, University of Leeds, Leeds, UK.<br /><searchLink fieldCode="AU" term="%22Sun+Y%22">Sun Y</searchLink>; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY, USA.<br /><searchLink fieldCode="AU" term="%22Brenowitz+M%22">Brenowitz M</searchLink>; Department of Biochemistry and Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY, USA.<br /><searchLink fieldCode="AU" term="%22Farquhar+ER%22">Farquhar ER</searchLink>; Department of Nutrition and Center for Synchrotron Biosciences, Case Western Reserve University, Cleveland, OH, USA.<br /><searchLink fieldCode="AU" term="%22Chance+MR%22">Chance MR</searchLink>; Department of Nutrition and Center for Synchrotron Biosciences, Case Western Reserve University, Cleveland, OH, USA.<br /><searchLink fieldCode="AU" term="%22Kuang+XC%22">Kuang XC</searchLink>; Department of Chemistry, Washington University in St Louis, St Louis, MO, USA.<br /><searchLink fieldCode="AU" term="%22Gross+ML%22">Gross ML</searchLink>; Department of Chemistry, Washington University in St Louis, St Louis, MO, USA.<br /><searchLink fieldCode="AU" term="%22Jones+LM%22">Jones LM</searchLink>; Department of Chemistry and Biochemistry, University of California, San Diego, San Diego, CA, USA.<br /><searchLink fieldCode="AU" term="%22Novak+P%22">Novak P</searchLink>; Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic.<br /><searchLink fieldCode="AU" term="%22Misra+SK%22">Misra SK</searchLink>; Department of BioMolecular Sciences, University of Mississippi, Oxford, MS, USA.<br /><searchLink fieldCode="AU" term="%22Sharp+JS%22">Sharp JS</searchLink>; Department of BioMolecular Sciences, University of Mississippi, Oxford, MS, USA. jsharp@olemiss.edu.
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