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Isolation of genome-predicted Caldatribacterium (Atribacterota) reveals pervasive microbial cultivation problem due to folate precipitation.

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Title: Isolation of genome-predicted Caldatribacterium (Atribacterota) reveals pervasive microbial cultivation problem due to folate precipitation.
Authors: Hedlund BP; School of Life Sciences, University of Nevada, Las Vegas, NV, USA. brian.hedlund@unlv.edu., Alvarado T; Department of Biology, California State University, San Bernardino, CA, USA., Sushenko NS; School of Life Sciences, University of Nevada, Las Vegas, NV, USA., Murphy TR; Department of Microbiology, Southern Illinois University, Carbondale, IL, USA., Torres A; Department of Biology, California State University, San Bernardino, CA, USA., Blocker AM; Department of Microbiology, Southern Illinois University, Carbondale, IL, USA., Lai D; School of Life Sciences, University of Nevada, Las Vegas, NV, USA., Mayali X; Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA., Seymour CO; School of Life Sciences, University of Nevada, Las Vegas, NV, USA.; Department of Pediatrics, University of California San Diego, La Jolla, CA, USA., Muok A; Institute for Biology, Leiden University, Leiden, BE, The Netherlands.; Centre for Microbial Cell Biology, Leiden University, Leiden, BE, The Netherlands., Xiao R; Pathobiology Department, Center for Host Microbe Interactions, University of Pennsylvania, Philadelphia, PA, USA., Levon E; Department of Microbiology, Southern Illinois University, Carbondale, IL, USA., Phoenix JT; Department of Microbiology, Southern Illinois University, Carbondale, IL, USA., Mazdra K; Department of Microbiology, Southern Illinois University, Carbondale, IL, USA., Sons T; Department of Microbiology, Southern Illinois University, Carbondale, IL, USA., Doss RK; School of Life Sciences, University of Nevada, Las Vegas, NV, USA., Vamvakas A; Department of Biology, California State University, San Bernardino, CA, USA., Camacho A; Department of Biology, California State University, San Bernardino, CA, USA., Ledesma J; Department of Biology, California State University, San Bernardino, CA, USA., Jiries J; Department of Biology, California State University, San Bernardino, CA, USA., Manriquez A; Department of Biology, California State University, San Bernardino, CA, USA., Martinez AR; Department of Biology, California State University, San Bernardino, CA, USA., Owerkowicz Z; Department of Biology, California State University, San Bernardino, CA, USA., Lacy J; Department of Biology, California State University, San Bernardino, CA, USA., Weber PK; Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA., Briegel A; Institute for Biology, Leiden University, Leiden, BE, The Netherlands.; Centre for Microbial Cell Biology, Leiden University, Leiden, BE, The Netherlands.; Integrative Structural Cell Biology Unit, Department of Structural Biology and Chemistry, Institut Pasteur, Paris, France., Moser DP; Division of Hydrologic Sciences, Desert Research Institute, Las Vegas, NV, USA., Pett-Ridge J; Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA.; Life & Environmental Sciences Department, University of California, Merced, CA, USA.; Innovative Genomics Institute, University of California, Berkeley, CA, USA., Dodsworth JA; Department of Biology, California State University, San Bernardino, CA, USA. JDodsworth@csusb.edu., Hamilton-Brehm SD; Department of Microbiology, Southern Illinois University, Carbondale, IL, USA. scott.hamilton-brehm@siu.edu.
Source: Nature communications [Nat Commun] 2026 Jun 11. Date of Electronic Publication: 2026 Jun 11.
Publication Type: Journal Article
Journal Info: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
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JDodsworth@csusb.edu.<br /><searchLink fieldCode="AU" term="%22Hamilton-Brehm+SD%22">Hamilton-Brehm SD</searchLink>; Department of Microbiology, Southern Illinois University, Carbondale, IL, USA. scott.hamilton-brehm@siu.edu.
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