Bladder pain induced by prolonged peripheral alpha 1A adrenoceptor stimulation involves the enhancement of transient receptor potential vanilloid 1 activity and an increase of urothelial adenosine triphosphate release.
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| Title: | Bladder pain induced by prolonged peripheral alpha 1A adrenoceptor stimulation involves the enhancement of transient receptor potential vanilloid 1 activity and an increase of urothelial adenosine triphosphate release. |
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| Authors: | Matos, R., Cordeiro, J. M., Coelho, A., Ferreira, S., Silva, C., Igawa, Y., Cruz, F., Charrua, A. |
| Source: | Acta Physiologica. Dec2016, Vol. 218 Issue 4, p265-275. 11p. |
| Subjects: | Visceral pain, Interstitial cystitis, Nociceptors, TRP channels, Alpha adrenoceptors, Adenosine triphosphatase, Therapeutics |
| Abstract: | Aim Pathophysiological mechanisms of chronic visceral pain ( CVP) are unknown. This study explores the association between the sympathetic system and bladder nociceptors activity by testing the effect of a prolonged adrenergic stimulation on transient receptor potential vanilloid 1 ( TRPV1) activity and on urothelial adenosine triphosphate ( ATP) release. Methods Female Wistar rats received saline, phenylephrine ( PHE), PHE + silodosin, PHE + naftopidil or PHE + prazosin. TRPV1 knockout and wild-type mice received saline or PHE. Visceral pain behaviour tests were performed before and after treatment. Cystometry was performed, during saline and capsaicin infusion. Fos immunoreactivity was assessed in L6 spinal cord segment. Human urothelial ATP release induced by mechanical and thermal stimulation was evaluated. Results Subcutaneous, but not intrathecal, PHE administration induced pain, which was reversed by silodosin, a selective alpha 1A adrenoceptor antagonist, but not by naftopidil, a relatively selective antagonist for alpha 1D adrenoceptor. Silodosin also reversed PHE-induced bladder hyperactivity and L6 spinal cord Fos expression. Thus, in subsequent experiments, only silodosin was used. Wild-type, but not TRPV1 knockout, mice exhibited phenylephrine-induced pain. Capsaicin induced a greater increase in voiding contractions in PHE-treated rats than in control animals, and silodosin reversed this effect. When treated with PHE, ATP release from human urothelial cells was enhanced either by mechanical stimulation or by lowering the thermal threshold of urothelial TRPV1, which becomes abnormally responsive at body temperature. Conclusion This study suggests that the activation of peripheral alpha 1A adrenoceptors induces CVP, probably through its interaction with TRPV1 and ATP release. [ABSTRACT FROM AUTHOR] |
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| Database: | Psychology and Behavioral Sciences Collection |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: pbh DbLabel: Psychology and Behavioral Sciences Collection An: 119354318 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Bladder pain induced by prolonged peripheral alpha 1A adrenoceptor stimulation involves the enhancement of transient receptor potential vanilloid 1 activity and an increase of urothelial adenosine triphosphate release. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Matos%2C+R%2E%22">Matos, R.</searchLink><br /><searchLink fieldCode="AR" term="%22Cordeiro%2C+J%2E+M%2E%22">Cordeiro, J. M.</searchLink><br /><searchLink fieldCode="AR" term="%22Coelho%2C+A%2E%22">Coelho, A.</searchLink><br /><searchLink fieldCode="AR" term="%22Ferreira%2C+S%2E%22">Ferreira, S.</searchLink><br /><searchLink fieldCode="AR" term="%22Silva%2C+C%2E%22">Silva, C.</searchLink><br /><searchLink fieldCode="AR" term="%22Igawa%2C+Y%2E%22">Igawa, Y.</searchLink><br /><searchLink fieldCode="AR" term="%22Cruz%2C+F%2E%22">Cruz, F.</searchLink><br /><searchLink fieldCode="AR" term="%22Charrua%2C+A%2E%22">Charrua, A.</searchLink> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Acta+Physiologica%22">Acta Physiologica</searchLink>. Dec2016, Vol. 218 Issue 4, p265-275. 11p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Visceral+pain%22">Visceral pain</searchLink><br /><searchLink fieldCode="DE" term="%22Interstitial+cystitis%22">Interstitial cystitis</searchLink><br /><searchLink fieldCode="DE" term="%22Nociceptors%22">Nociceptors</searchLink><br /><searchLink fieldCode="DE" term="%22TRP+channels%22">TRP channels</searchLink><br /><searchLink fieldCode="DE" term="%22Alpha+adrenoceptors%22">Alpha adrenoceptors</searchLink><br /><searchLink fieldCode="DE" term="%22Adenosine+triphosphatase%22">Adenosine triphosphatase</searchLink><br /><searchLink fieldCode="DE" term="%22Therapeutics%22">Therapeutics</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Aim Pathophysiological mechanisms of chronic visceral pain ( CVP) are unknown. This study explores the association between the sympathetic system and bladder nociceptors activity by testing the effect of a prolonged adrenergic stimulation on transient receptor potential vanilloid 1 ( TRPV1) activity and on urothelial adenosine triphosphate ( ATP) release. Methods Female Wistar rats received saline, phenylephrine ( PHE), PHE + silodosin, PHE + naftopidil or PHE + prazosin. TRPV1 knockout and wild-type mice received saline or PHE. Visceral pain behaviour tests were performed before and after treatment. Cystometry was performed, during saline and capsaicin infusion. Fos immunoreactivity was assessed in L6 spinal cord segment. Human urothelial ATP release induced by mechanical and thermal stimulation was evaluated. Results Subcutaneous, but not intrathecal, PHE administration induced pain, which was reversed by silodosin, a selective alpha 1A adrenoceptor antagonist, but not by naftopidil, a relatively selective antagonist for alpha 1D adrenoceptor. Silodosin also reversed PHE-induced bladder hyperactivity and L6 spinal cord Fos expression. Thus, in subsequent experiments, only silodosin was used. Wild-type, but not TRPV1 knockout, mice exhibited phenylephrine-induced pain. Capsaicin induced a greater increase in voiding contractions in PHE-treated rats than in control animals, and silodosin reversed this effect. When treated with PHE, ATP release from human urothelial cells was enhanced either by mechanical stimulation or by lowering the thermal threshold of urothelial TRPV1, which becomes abnormally responsive at body temperature. Conclusion This study suggests that the activation of peripheral alpha 1A adrenoceptors induces CVP, probably through its interaction with TRPV1 and ATP release. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Acta Physiologica is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1111/apha.12744 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 11 StartPage: 265 Subjects: – SubjectFull: Visceral pain Type: general – SubjectFull: Interstitial cystitis Type: general – SubjectFull: Nociceptors Type: general – SubjectFull: TRP channels Type: general – SubjectFull: Alpha adrenoceptors Type: general – SubjectFull: Adenosine triphosphatase Type: general – SubjectFull: Therapeutics Type: general Titles: – TitleFull: Bladder pain induced by prolonged peripheral alpha 1A adrenoceptor stimulation involves the enhancement of transient receptor potential vanilloid 1 activity and an increase of urothelial adenosine triphosphate release. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Matos, R. – PersonEntity: Name: NameFull: Cordeiro, J. M. – PersonEntity: Name: NameFull: Coelho, A. – PersonEntity: Name: NameFull: Ferreira, S. – PersonEntity: Name: NameFull: Silva, C. – PersonEntity: Name: NameFull: Igawa, Y. – PersonEntity: Name: NameFull: Cruz, F. – PersonEntity: Name: NameFull: Charrua, A. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 12 Text: Dec2016 Type: published Y: 2016 Identifiers: – Type: issn-print Value: 17481708 Numbering: – Type: volume Value: 218 – Type: issue Value: 4 Titles: – TitleFull: Acta Physiologica Type: main |
| ResultId | 1 |
