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High sensitivity of spontaneous spike frequency to sodium leak current in a Lymnaea pacemaker neuron.

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Title: High sensitivity of spontaneous spike frequency to sodium leak current in a Lymnaea pacemaker neuron.
Authors: Lu, T. Z., Kostelecki, W., Sun, C. L. F., Dong, N., Pérez Velázquez, J. L., Feng, Z.‐P., Poirazi, Panayiota
Source: European Journal of Neuroscience. Dec2016, Vol. 44 Issue 12, p3011-3022. 12p. 1 Chart, 7 Graphs.
Subjects: Action potentials, Voltage-gated ion channels, Neural physiology, Cardiac pacemakers, Lymnaea, Patch-clamp techniques (Electrophysiology)
Abstract: The spontaneous rhythmic firing of action potentials in pacemaker neurons depends on the biophysical properties of voltage-gated ion channels and background leak currents. The background leak current includes a large K+ and a small Na+ component. We previously reported that a Na+-leak current via U-type channels is required to generate spontaneous action potential firing in the identified respiratory pacemaker neuron, RPeD1, in the freshwater pond snail Lymnaea stagnalis. We further investigated the functional significance of the background Na+ current in rhythmic spiking of RPeD1 neurons. Whole-cell patch-clamp recording and computational modeling approaches were carried out in isolated RPeD1 neurons. The whole-cell current of the major ion channel components in RPeD1 neurons were characterized, and a conductance-based computational model of the rhythmic pacemaker activity was simulated with the experimental measurements. We found that the spiking rate is more sensitive to changes in the Na+ leak current as compared to the K+ leak current, suggesting a robust function of Na+ leak current in regulating spontaneous neuronal firing activity. Our study provides new insight into our current understanding of the role of Na+ leak current in intrinsic properties of pacemaker neurons. [ABSTRACT FROM AUTHOR]
Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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DbLabel: Psychology and Behavioral Sciences Collection
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PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
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  Label: Title
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  Data: High sensitivity of spontaneous spike frequency to sodium leak current in a Lymnaea pacemaker neuron.
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  Data: <searchLink fieldCode="AR" term="%22Lu%2C+T%2E+Z%2E%22">Lu, T. Z.</searchLink><br /><searchLink fieldCode="AR" term="%22Kostelecki%2C+W%2E%22">Kostelecki, W.</searchLink><br /><searchLink fieldCode="AR" term="%22Sun%2C+C%2E+L%2E+F%2E%22">Sun, C. L. F.</searchLink><br /><searchLink fieldCode="AR" term="%22Dong%2C+N%2E%22">Dong, N.</searchLink><br /><searchLink fieldCode="AR" term="%22Pérez+Velázquez%2C+J%2E+L%2E%22">Pérez Velázquez, J. L.</searchLink><br /><searchLink fieldCode="AR" term="%22Feng%2C+Z%2E‐P%2E%22">Feng, Z.‐P.</searchLink><br /><searchLink fieldCode="AR" term="%22Poirazi%2C+Panayiota%22">Poirazi, Panayiota</searchLink>
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  Data: <searchLink fieldCode="JN" term="%22European+Journal+of+Neuroscience%22">European Journal of Neuroscience</searchLink>. Dec2016, Vol. 44 Issue 12, p3011-3022. 12p. 1 Chart, 7 Graphs.
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  Data: <searchLink fieldCode="DE" term="%22Action+potentials%22">Action potentials</searchLink><br /><searchLink fieldCode="DE" term="%22Voltage-gated+ion+channels%22">Voltage-gated ion channels</searchLink><br /><searchLink fieldCode="DE" term="%22Neural+physiology%22">Neural physiology</searchLink><br /><searchLink fieldCode="DE" term="%22Cardiac+pacemakers%22">Cardiac pacemakers</searchLink><br /><searchLink fieldCode="DE" term="%22Lymnaea%22">Lymnaea</searchLink><br /><searchLink fieldCode="DE" term="%22Patch-clamp+techniques+%28Electrophysiology%29%22">Patch-clamp techniques (Electrophysiology)</searchLink>
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  Label: Abstract
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  Data: The spontaneous rhythmic firing of action potentials in pacemaker neurons depends on the biophysical properties of voltage-gated ion channels and background leak currents. The background leak current includes a large K+ and a small Na+ component. We previously reported that a Na+-leak current via U-type channels is required to generate spontaneous action potential firing in the identified respiratory pacemaker neuron, RPeD1, in the freshwater pond snail Lymnaea stagnalis. We further investigated the functional significance of the background Na+ current in rhythmic spiking of RPeD1 neurons. Whole-cell patch-clamp recording and computational modeling approaches were carried out in isolated RPeD1 neurons. The whole-cell current of the major ion channel components in RPeD1 neurons were characterized, and a conductance-based computational model of the rhythmic pacemaker activity was simulated with the experimental measurements. We found that the spiking rate is more sensitive to changes in the Na+ leak current as compared to the K+ leak current, suggesting a robust function of Na+ leak current in regulating spontaneous neuronal firing activity. Our study provides new insight into our current understanding of the role of Na+ leak current in intrinsic properties of pacemaker neurons. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1111/ejn.13426
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        Text: English
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        Type: general
      – SubjectFull: Voltage-gated ion channels
        Type: general
      – SubjectFull: Neural physiology
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      – SubjectFull: Cardiac pacemakers
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      – SubjectFull: Lymnaea
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      – SubjectFull: Patch-clamp techniques (Electrophysiology)
        Type: general
    Titles:
      – TitleFull: High sensitivity of spontaneous spike frequency to sodium leak current in a Lymnaea pacemaker neuron.
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            NameFull: Lu, T. Z.
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            NameFull: Dong, N.
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            NameFull: Pérez Velázquez, J. L.
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              Text: Dec2016
              Type: published
              Y: 2016
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