RAD51 bypasses the CMG helicase to promote replication fork reversal.
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| Title: | RAD51 bypasses the CMG helicase to promote replication fork reversal. |
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| Authors: | Liu, Wenpeng, Saito, Yuichiro, Jackson, Jessica, Bhowmick, Rahul, Kanemaki, Masato T., Vindigni, Alessandro, Cortez, David |
| Source: | Science (pre-March 2025). 4/28/2023, Vol. 380 Issue 6643, p382-387. 6p. 3 Diagrams. |
| Subjects: | DNA helicases, DNA replication, DNA synthesis, Recombinases |
| Abstract: | Replication fork reversal safeguards genome integrity as a replication stress response. DNA translocases and the RAD51 recombinase catalyze reversal. However, it remains unknown why RAD51 is required and what happens to the replication machinery during reversal. We find that RAD51 uses its strand exchange activity to circumvent the replicative helicase, which remains bound to the stalled fork. RAD51 is not required for fork reversal if the helicase is unloaded. Thus, we propose that RAD51 creates a parental DNA duplex behind the helicase that is used as a substrate by the DNA translocases for branch migration to create a reversed fork structure. Our data explain how fork reversal happens while maintaining the helicase in a position poised to restart DNA synthesis and complete genome duplication. [ABSTRACT FROM AUTHOR] |
| Copyright of Science (pre-March 2025) is the property of American Association for the Advancement of Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Psychology and Behavioral Sciences Collection |
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