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Cell type-specific regulation of RE-1 silencing transcription factor (REST) target genes.

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Title: Cell type-specific regulation of RE-1 silencing transcription factor (REST) target genes.
Authors: Hohl, Mathias (AUTHOR), Thiel, Gerald (AUTHOR)
Source: European Journal of Neuroscience. Nov2005, Vol. 22 Issue 9, p2216-2230. 15p. 6 Diagrams, 4 Graphs.
Subjects: Transcription factors, Gene silencing, Neurons, Neuroendocrine cells, Histone deacetylases, Gene expression, Chromatin, Transcription factor REST
Abstract: RE-1 silencing transcription factor (REST) is a transcriptional repressor that represses neuronal gene transcription in non-neuronal cells. REST target genes are expressed in neurons and in neuroendocrine cells. Here, we show that treatment with the histone deacetylase inhibitor trichostatin A (TSA) or expression of a mutant of REST (DP-REST:ER) that contains a transcriptional activation domain enhanced expression of the REST target genes encoding synaptophysin and secretogranin II in neuronal as well as in neuroendocrine cells. These data indicate that the synaptophysin and secretogranin II genes are similarly regulated in neuronal and neuroendocrine cells. In contrast, expression of the connexin36 gene was inducible by TSA or DP-REST:ER only in pancreatic α and β cells, but not in neuronal and pituitary cells, indicating that transcriptional repression by REST functions in a cell type-specific manner. Expression of the BDNF and GluR2 genes, both described as targets of REST, was not induced by either TSA or expression of DP-REST:ER in neuronal or neuroendocrine cells. Chromatin immunoprecipitation experiments using antibodies directed against methylated histone H3Lys4 or H3Lys9 showed a perfect correlation between expression of REST target genes in different cell types and nucleosomal modifications that distinguish active from inactive genes. We conclude that the cell type-specific microenvironment, in particular the cell type-specific structure of the chromatin, is crucial for the ability of REST to control gene transcription. [ABSTRACT FROM AUTHOR]
Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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  Label: Title
  Group: Ti
  Data: Cell type-specific regulation of RE-1 silencing transcription factor (REST) target genes.
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  Data: <searchLink fieldCode="AR" term="%22Hohl%2C+Mathias%22">Hohl, Mathias</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Thiel%2C+Gerald%22">Thiel, Gerald</searchLink> (AUTHOR)
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  Data: <searchLink fieldCode="JN" term="%22European+Journal+of+Neuroscience%22">European Journal of Neuroscience</searchLink>. Nov2005, Vol. 22 Issue 9, p2216-2230. 15p. 6 Diagrams, 4 Graphs.
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  Data: <searchLink fieldCode="DE" term="%22Transcription+factors%22">Transcription factors</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+silencing%22">Gene silencing</searchLink><br /><searchLink fieldCode="DE" term="%22Neurons%22">Neurons</searchLink><br /><searchLink fieldCode="DE" term="%22Neuroendocrine+cells%22">Neuroendocrine cells</searchLink><br /><searchLink fieldCode="DE" term="%22Histone+deacetylases%22">Histone deacetylases</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+expression%22">Gene expression</searchLink><br /><searchLink fieldCode="DE" term="%22Chromatin%22">Chromatin</searchLink><br /><searchLink fieldCode="DE" term="%22Transcription+factor+REST%22">Transcription factor REST</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: RE-1 silencing transcription factor (REST) is a transcriptional repressor that represses neuronal gene transcription in non-neuronal cells. REST target genes are expressed in neurons and in neuroendocrine cells. Here, we show that treatment with the histone deacetylase inhibitor trichostatin A (TSA) or expression of a mutant of REST (DP-REST:ER) that contains a transcriptional activation domain enhanced expression of the REST target genes encoding synaptophysin and secretogranin II in neuronal as well as in neuroendocrine cells. These data indicate that the synaptophysin and secretogranin II genes are similarly regulated in neuronal and neuroendocrine cells. In contrast, expression of the connexin36 gene was inducible by TSA or DP-REST:ER only in pancreatic α and β cells, but not in neuronal and pituitary cells, indicating that transcriptional repression by REST functions in a cell type-specific manner. Expression of the BDNF and GluR2 genes, both described as targets of REST, was not induced by either TSA or expression of DP-REST:ER in neuronal or neuroendocrine cells. Chromatin immunoprecipitation experiments using antibodies directed against methylated histone H3Lys4 or H3Lys9 showed a perfect correlation between expression of REST target genes in different cell types and nucleosomal modifications that distinguish active from inactive genes. We conclude that the cell type-specific microenvironment, in particular the cell type-specific structure of the chromatin, is crucial for the ability of REST to control gene transcription. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=pbh&AN=18712420
RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1111/j.1460-9568.2005.04404.x
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      – Code: eng
        Text: English
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        Type: general
      – SubjectFull: Gene silencing
        Type: general
      – SubjectFull: Neurons
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      – SubjectFull: Neuroendocrine cells
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      – SubjectFull: Histone deacetylases
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      – SubjectFull: Gene expression
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      – SubjectFull: Chromatin
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      – SubjectFull: Transcription factor REST
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      – TitleFull: Cell type-specific regulation of RE-1 silencing transcription factor (REST) target genes.
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            NameFull: Hohl, Mathias
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              M: 11
              Text: Nov2005
              Type: published
              Y: 2005
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