View in EDS HTML Full Text PDF Full Text

Exercise affects energy metabolism and neural plasticity-related proteins in the hippocampus as revealed by proteomic analysis.

Saved in:
Bibliographic Details
Title: Exercise affects energy metabolism and neural plasticity-related proteins in the hippocampus as revealed by proteomic analysis.
Authors: Ding, Qinxue (AUTHOR), Vaynman, Shoshanna (AUTHOR), Souda, Puneet (AUTHOR), Whitelegge, Julian P. (AUTHOR), Gomez‐Pinilla, Fernando (AUTHOR)
Source: European Journal of Neuroscience. Sep2006, Vol. 24 Issue 5, p1265-1276. 12p. 3 Diagrams, 4 Charts, 1 Graph.
Subjects: Molecular chaperones, Cytoskeletal proteins, Energy metabolism, Neuroplasticity, Proteomics
Abstract: Studies were conducted to evaluate the effect of a brief voluntary exercise period on the expression pattern and post-translational modification of multiple protein classes in the rat hippocampus using proteomics. An analysis of 80 protein spots of relative high abundance on two-dimensional gels revealed that approximately 90% of the proteins identified were associated with energy metabolism and synaptic plasticity. Exercise up-regulated proteins involved in four aspects of energy metabolism, i.e. glycolysis, ATP synthesis, ATP transduction and glutamate turnover. Specifically, we found increases in fructose-bisphosphate aldolase C, phosphoglycerate kinase 1, mitochondrial ATP synthase, ubiquitous mitochondrial creatine kinase and glutamate dehydrogenase 1. Exercise also up-regulated specific synaptic-plasticity-related proteins, the cytoskeletal protein α-internexin and molecular chaperones (chaperonin-containing TCP-1, neuronal protein 22, heat shock 60-kDa protein 1 and heat shock protein 8). Western blot was used to confirm the direction and magnitude of change in ubiquitous mitochondrial creatine kinase, an enzyme essential for transducing mitochondrial-derived ATP to sites of high-energy demand such as the synapse. Protein phosphorylation visualized by Pro-Q Diamond fluorescent staining showed that neurofilament light polypeptide, glial fibrillary acidic protein, heat shock protein 8 and transcriptional activator protein pur-alpha were more intensely phosphorylated with exercise as compared with sedentary control levels. Our results, together with the fact that most of the proteins that we found to be up-regulated have been implicated in cognitive function, support a mechanism by which exercise uses processes of energy metabolism and synaptic plasticity to promote brain health. [ABSTRACT FROM AUTHOR]
Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
Full text is not displayed to guests.
FullText Links:
  – Type: pdflink
Text:
  Availability: 1
Header DbId: pbh
DbLabel: Psychology and Behavioral Sciences Collection
An: 22375493
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Exercise affects energy metabolism and neural plasticity-related proteins in the hippocampus as revealed by proteomic analysis.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Ding%2C+Qinxue%22">Ding, Qinxue</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Vaynman%2C+Shoshanna%22">Vaynman, Shoshanna</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Souda%2C+Puneet%22">Souda, Puneet</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Whitelegge%2C+Julian+P%2E%22">Whitelegge, Julian P.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Gomez‐Pinilla%2C+Fernando%22">Gomez‐Pinilla, Fernando</searchLink> (AUTHOR)
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22European+Journal+of+Neuroscience%22">European Journal of Neuroscience</searchLink>. Sep2006, Vol. 24 Issue 5, p1265-1276. 12p. 3 Diagrams, 4 Charts, 1 Graph.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Molecular+chaperones%22">Molecular chaperones</searchLink><br /><searchLink fieldCode="DE" term="%22Cytoskeletal+proteins%22">Cytoskeletal proteins</searchLink><br /><searchLink fieldCode="DE" term="%22Energy+metabolism%22">Energy metabolism</searchLink><br /><searchLink fieldCode="DE" term="%22Neuroplasticity%22">Neuroplasticity</searchLink><br /><searchLink fieldCode="DE" term="%22Proteomics%22">Proteomics</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Studies were conducted to evaluate the effect of a brief voluntary exercise period on the expression pattern and post-translational modification of multiple protein classes in the rat hippocampus using proteomics. An analysis of 80 protein spots of relative high abundance on two-dimensional gels revealed that approximately 90% of the proteins identified were associated with energy metabolism and synaptic plasticity. Exercise up-regulated proteins involved in four aspects of energy metabolism, i.e. glycolysis, ATP synthesis, ATP transduction and glutamate turnover. Specifically, we found increases in fructose-bisphosphate aldolase C, phosphoglycerate kinase 1, mitochondrial ATP synthase, ubiquitous mitochondrial creatine kinase and glutamate dehydrogenase 1. Exercise also up-regulated specific synaptic-plasticity-related proteins, the cytoskeletal protein α-internexin and molecular chaperones (chaperonin-containing TCP-1, neuronal protein 22, heat shock 60-kDa protein 1 and heat shock protein 8). Western blot was used to confirm the direction and magnitude of change in ubiquitous mitochondrial creatine kinase, an enzyme essential for transducing mitochondrial-derived ATP to sites of high-energy demand such as the synapse. Protein phosphorylation visualized by Pro-Q Diamond fluorescent staining showed that neurofilament light polypeptide, glial fibrillary acidic protein, heat shock protein 8 and transcriptional activator protein pur-alpha were more intensely phosphorylated with exercise as compared with sedentary control levels. Our results, together with the fact that most of the proteins that we found to be up-regulated have been implicated in cognitive function, support a mechanism by which exercise uses processes of energy metabolism and synaptic plasticity to promote brain health. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=pbh&AN=22375493
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1111/j.1460-9568.2006.05026.x
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 12
        StartPage: 1265
    Subjects:
      – SubjectFull: Molecular chaperones
        Type: general
      – SubjectFull: Cytoskeletal proteins
        Type: general
      – SubjectFull: Energy metabolism
        Type: general
      – SubjectFull: Neuroplasticity
        Type: general
      – SubjectFull: Proteomics
        Type: general
    Titles:
      – TitleFull: Exercise affects energy metabolism and neural plasticity-related proteins in the hippocampus as revealed by proteomic analysis.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Ding, Qinxue
      – PersonEntity:
          Name:
            NameFull: Vaynman, Shoshanna
      – PersonEntity:
          Name:
            NameFull: Souda, Puneet
      – PersonEntity:
          Name:
            NameFull: Whitelegge, Julian P.
      – PersonEntity:
          Name:
            NameFull: Gomez‐Pinilla, Fernando
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 09
              Text: Sep2006
              Type: published
              Y: 2006
          Identifiers:
            – Type: issn-print
              Value: 0953816X
          Numbering:
            – Type: volume
              Value: 24
            – Type: issue
              Value: 5
          Titles:
            – TitleFull: European Journal of Neuroscience
              Type: main
ResultId 1