Acetylcholine-induced Phosphorylation in Isolated Outer Hair Cells.
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| Title: | Acetylcholine-induced Phosphorylation in Isolated Outer Hair Cells. |
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| Authors: | Szõnyi, Magdolna, Csermely, Péter, Sziklai, István |
| Source: | Acta Oto-Laryngologica. 4/22/99, Vol. 119 Issue 2, p185-188. 4p. |
| Subjects: | Hair cells, Acetylcholine |
| Abstract: | Two groups of isolated, surviving outer hair cells (OHCs) of guinea pig cochleas (n=20, for each group) were treated with 10 μM acetylcholine or acetylcholine plus strichnine (an α9 nAChR antagonist), respectively, under short-term tissue culture conditions. The protein content of the cell homogenates was separated by SDS-polyacrylamide gel electrophoresis, Western blotted and labelled with an antibody against phosphoserine residues. Signals were detected using the ECL system. Acetylcholine challenge of the OHCs resulted in a difference in the pattern of phosphorylated proteins from those of strichnine pretreated cells. A 220 kDa and a 120 kDa protein expressed a more intense phosphorylated state in the ACh group compared with the ACh plus strichnine group. The 220 kDa phosphoprotein is in the range of the cytoskeletal protein β-fodrin, whereas the 120 kDa fraction is similar to α-fodrin or an ankyrin isoform. Phosphorylation of proteins due to activation of the AChR by agonist can play a role in the signalling mechanism between receptor activation and increase in the electromotile capability of isolated OHCs. [ABSTRACT FROM AUTHOR] |
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| Database: | Psychology and Behavioral Sciences Collection |
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