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In vitro cholesteatoma growth and secretion of cytokines.

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Title: In vitro cholesteatoma growth and secretion of cytokines.
Authors: Helgaland, Tore (AUTHOR), Engelen, Bart (AUTHOR), Olsnes, Carla (AUTHOR), Aarstad, Hans Jørgen (AUTHOR), Vassbotn, Flemming S. (AUTHOR)
Source: Acta Oto-Laryngologica. Jul2010, Vol. 130 Issue 7, p815-819. 5p. 2 Color Photographs, 3 Graphs.
Subjects: Cholesteatoma, Etiology of diseases, Skin, Cytokines, Otitis media, Comparative studies, Patients
Abstract: Conclusion: Our results show a significant difference between skin and cholesteatoma biology in vitro. Objectives: Cholesteatoma disease is a process of destruction characterized by uncontrolled growth of squamous epithelial cells in the middle ear or temporal bone. The pathophysiology behind the cholesteatoma development is controversial, and the mechanisms driving the cholesteatoma growth, migration and destructive properties is still unclear. We aimed to provide a method to study the effect of various compounds on cholesteatoma and skin tissue growth, as well as to further investigate the biological differences between normal skin and cholesteatoma tissue. Methods: We have established a method to study cholesteatoma biopsy tissue in vitro. Cholesteatoma tissues from patients undergoing surgery for chronic otitis were grown in culture medium and compared to growth patterns and behaviour of normal retroauricular skin. Conditioned medium was analysed for various secreted cytokines. Results: We found a radial outgrowth of keratinocyte epithelium from the circular biopsies. After 5 days of culture we found a significant growth of both cholesteatoma and skin-derived cells. Cholesteatoma samples showed higher growth rate as compared with skin control cultures from the same patient. Moreover, the cholesteatoma cells showed higher production of monocyte chemoattractant protein-1 (MCP-1) and interleukin (IL)-6 as compared with normal skin. [ABSTRACT FROM AUTHOR]
Copyright of Acta Oto-Laryngologica is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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  Data: In vitro cholesteatoma growth and secretion of cytokines.
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  Data: <searchLink fieldCode="AR" term="%22Helgaland%2C+Tore%22">Helgaland, Tore</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Engelen%2C+Bart%22">Engelen, Bart</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Olsnes%2C+Carla%22">Olsnes, Carla</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Aarstad%2C+Hans+Jørgen%22">Aarstad, Hans Jørgen</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Vassbotn%2C+Flemming+S%2E%22">Vassbotn, Flemming S.</searchLink> (AUTHOR)
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  Data: <searchLink fieldCode="JN" term="%22Acta+Oto-Laryngologica%22">Acta Oto-Laryngologica</searchLink>. Jul2010, Vol. 130 Issue 7, p815-819. 5p. 2 Color Photographs, 3 Graphs.
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  Data: <searchLink fieldCode="DE" term="%22Cholesteatoma%22">Cholesteatoma</searchLink><br /><searchLink fieldCode="DE" term="%22Etiology+of+diseases%22">Etiology of diseases</searchLink><br /><searchLink fieldCode="DE" term="%22Skin%22">Skin</searchLink><br /><searchLink fieldCode="DE" term="%22Cytokines%22">Cytokines</searchLink><br /><searchLink fieldCode="DE" term="%22Otitis+media%22">Otitis media</searchLink><br /><searchLink fieldCode="DE" term="%22Comparative+studies%22">Comparative studies</searchLink><br /><searchLink fieldCode="DE" term="%22Patients%22">Patients</searchLink>
– Name: Abstract
  Label: Abstract
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  Data: Conclusion: Our results show a significant difference between skin and cholesteatoma biology in vitro. Objectives: Cholesteatoma disease is a process of destruction characterized by uncontrolled growth of squamous epithelial cells in the middle ear or temporal bone. The pathophysiology behind the cholesteatoma development is controversial, and the mechanisms driving the cholesteatoma growth, migration and destructive properties is still unclear. We aimed to provide a method to study the effect of various compounds on cholesteatoma and skin tissue growth, as well as to further investigate the biological differences between normal skin and cholesteatoma tissue. Methods: We have established a method to study cholesteatoma biopsy tissue in vitro. Cholesteatoma tissues from patients undergoing surgery for chronic otitis were grown in culture medium and compared to growth patterns and behaviour of normal retroauricular skin. Conditioned medium was analysed for various secreted cytokines. Results: We found a radial outgrowth of keratinocyte epithelium from the circular biopsies. After 5 days of culture we found a significant growth of both cholesteatoma and skin-derived cells. Cholesteatoma samples showed higher growth rate as compared with skin control cultures from the same patient. Moreover, the cholesteatoma cells showed higher production of monocyte chemoattractant protein-1 (MCP-1) and interleukin (IL)-6 as compared with normal skin. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Acta Oto-Laryngologica is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.3109/00016480903413657
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        Type: general
      – SubjectFull: Etiology of diseases
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              Text: Jul2010
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