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Erythropoietin exerts anti-apoptotic effects on rat microglial cells in vitro.

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Title: Erythropoietin exerts anti-apoptotic effects on rat microglial cells in vitro.
Authors: Vairano, Mauro (AUTHOR), Russo, Cinzia Dello (AUTHOR), Pozzoli, Giacomo (AUTHOR), Battaglia, Alessandra (AUTHOR), Scambia, Giovanni (AUTHOR), Tringali, Giuseppe (AUTHOR), Aloe‐Spiriti, Maria Antonietta (AUTHOR), Preziosi, Paolo (AUTHOR), Navarra, Pierluigi (AUTHOR)
Source: European Journal of Neuroscience. Aug2002, Vol. 16 Issue 4, p584-592. 9p.
Subjects: Erythropoietin, Apoptosis, Cell proliferation
Abstract: Abstract Erythropoietin (EPO), a renal cytokine regulating haematopoiesis, is also produced by different cell types within the central nervous system, where it acts via the activation of specific receptors. Current evidence shows that EPO exerts neurotrophic and neuroprotective activities in different in vivo and in vitro models of brain damage. In the present study we investigated the effects of EPO on primary cultures of rat cortical microglia and astrocytes. We found that: (i) EPO exerted a marked stimulatory effect on microglial cell viability, assessed through the MTS assay, whereas astrocytes were almost unaffected; (ii) the cytokine increased microglial cell population size in a concentration-dependent manner; however, as microglia cultures undergo spontaneous apoptosis after separation from astrocytes, the apparent effect on cell proliferation could be attributed to EPO antagonism of normal apoptosis; (iii) subsequent flow cytometry analysis on microglial cells demonstrated both the trophic role of factor(s) released by astrocytes in mixed cultures, and the putative anti-apoptotic action of EPO; (iv) the latter was further confirmed through the assessment of gene expression of anti- and pro-apoptotic factors, which showed that EPO is able to shift the Bcl : Bax ratio towards a net anti-apoptotic effect; (v) EPO did not affect the pro-inflammatory function of microglial cells. [ABSTRACT FROM AUTHOR]
Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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  Data: Erythropoietin exerts anti-apoptotic effects on rat microglial cells in vitro.
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  Data: <searchLink fieldCode="AR" term="%22Vairano%2C+Mauro%22">Vairano, Mauro</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Russo%2C+Cinzia+Dello%22">Russo, Cinzia Dello</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Pozzoli%2C+Giacomo%22">Pozzoli, Giacomo</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Battaglia%2C+Alessandra%22">Battaglia, Alessandra</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Scambia%2C+Giovanni%22">Scambia, Giovanni</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Tringali%2C+Giuseppe%22">Tringali, Giuseppe</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Aloe‐Spiriti%2C+Maria+Antonietta%22">Aloe‐Spiriti, Maria Antonietta</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Preziosi%2C+Paolo%22">Preziosi, Paolo</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Navarra%2C+Pierluigi%22">Navarra, Pierluigi</searchLink> (AUTHOR)
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  Data: <searchLink fieldCode="JN" term="%22European+Journal+of+Neuroscience%22">European Journal of Neuroscience</searchLink>. Aug2002, Vol. 16 Issue 4, p584-592. 9p.
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  Data: <searchLink fieldCode="DE" term="%22Erythropoietin%22">Erythropoietin</searchLink><br /><searchLink fieldCode="DE" term="%22Apoptosis%22">Apoptosis</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+proliferation%22">Cell proliferation</searchLink>
– Name: Abstract
  Label: Abstract
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  Data: Abstract Erythropoietin (EPO), a renal cytokine regulating haematopoiesis, is also produced by different cell types within the central nervous system, where it acts via the activation of specific receptors. Current evidence shows that EPO exerts neurotrophic and neuroprotective activities in different in vivo and in vitro models of brain damage. In the present study we investigated the effects of EPO on primary cultures of rat cortical microglia and astrocytes. We found that: (i) EPO exerted a marked stimulatory effect on microglial cell viability, assessed through the MTS assay, whereas astrocytes were almost unaffected; (ii) the cytokine increased microglial cell population size in a concentration-dependent manner; however, as microglia cultures undergo spontaneous apoptosis after separation from astrocytes, the apparent effect on cell proliferation could be attributed to EPO antagonism of normal apoptosis; (iii) subsequent flow cytometry analysis on microglial cells demonstrated both the trophic role of factor(s) released by astrocytes in mixed cultures, and the putative anti-apoptotic action of EPO; (iv) the latter was further confirmed through the assessment of gene expression of anti- and pro-apoptotic factors, which showed that EPO is able to shift the Bcl : Bax ratio towards a net anti-apoptotic effect; (v) EPO did not affect the pro-inflammatory function of microglial cells. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1046/j.1460-9568.2002.02125.x
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              Text: Aug2002
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