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Pistacia chinensis Inhibits NO Production and Upregulates HO-1 Induction via PI-3K/Akt Pathway in LPS Stimulated Macrophage Cells.

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Title: Pistacia chinensis Inhibits NO Production and Upregulates HO-1 Induction via PI-3K/Akt Pathway in LPS Stimulated Macrophage Cells.
Authors: Yayeh, Taddesse, Hong, Mei, Jia, Qi, Lee, Young-Chul, Kim, Hyun-Jin, Hyun, Eujin, Kim, Tae-Wan, Rhee, Man Hee
Source: American Journal of Chinese Medicine. 2012, Vol. 40 Issue 5, p1085-1097. 13p. 2 Black and White Photographs, 1 Chart, 2 Graphs.
Subjects: Antioxidant analysis, RNA analysis, Lipid analysis, Phenol analysis, Animal experimentation, Anti-inflammatory agents, Bark, Cell culture, Macrophages, Medicinal plants, Methanol, Nitric oxide, Oxidoreductases, Polymerase chain reaction, T-test (Statistics), Western immunoblotting, Plant extracts, Lipopolysaccharides, Descriptive statistics
Abstract: Pistacia chinensis has been used for various purposes in China including as an understock for grafting Pistacia vera. However, little attention was given to its health promoting effects. Therefore, in this study, we investigated the effect of Pistacia chinensis methanolic extract (PCME) containing resorcinol class of phenolic lipids on pro-inflammatory mediators and heme oxygenase-1(HO-1) in lipopolysaccharide stimulated RAW264.7 cells. While PCME (2.5-10 μg/ml) inhibited mRNA expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and interleukin (IL)-6, it up-regulated HO-1 expression. Likewise, PCME inhibited iNOS protein expression, but not COX-2, and reduced nitric oxide (NO) release. Moreover, Phosphorylated c-Jun N-terminal Kinase (JNK) was attenuated dose-dependently in PCME pre-treated RAW264.7 cells. In addition, PCME up-regulated HO-1 protein expression was diminished by pre-treatment of PI-3K inhibitor. Furthermore, nuclear factor erythroid 2 related factor 2 (Nrf2) repressor was attenuated time-dependently during PCME treatment. Taken together, our study showed (for the first time) that PCME inhibited NO production and up-regulated HO-1 induction via PI-3K/Akt pathway, suggesting the role of Pistacia chinensis as potential sources of anti-inflammatory and antioxidant natural compounds. [ABSTRACT FROM AUTHOR]
Copyright of American Journal of Chinese Medicine is the property of World Scientific Publishing Company and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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  Label: Title
  Group: Ti
  Data: Pistacia chinensis Inhibits NO Production and Upregulates HO-1 Induction via PI-3K/Akt Pathway in LPS Stimulated Macrophage Cells.
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  Label: Authors
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  Data: <searchLink fieldCode="AR" term="%22Yayeh%2C+Taddesse%22">Yayeh, Taddesse</searchLink><br /><searchLink fieldCode="AR" term="%22Hong%2C+Mei%22">Hong, Mei</searchLink><br /><searchLink fieldCode="AR" term="%22Jia%2C+Qi%22">Jia, Qi</searchLink><br /><searchLink fieldCode="AR" term="%22Lee%2C+Young-Chul%22">Lee, Young-Chul</searchLink><br /><searchLink fieldCode="AR" term="%22Kim%2C+Hyun-Jin%22">Kim, Hyun-Jin</searchLink><br /><searchLink fieldCode="AR" term="%22Hyun%2C+Eujin%22">Hyun, Eujin</searchLink><br /><searchLink fieldCode="AR" term="%22Kim%2C+Tae-Wan%22">Kim, Tae-Wan</searchLink><br /><searchLink fieldCode="AR" term="%22Rhee%2C+Man+Hee%22">Rhee, Man Hee</searchLink>
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  Data: <searchLink fieldCode="JN" term="%22American+Journal+of+Chinese+Medicine%22">American Journal of Chinese Medicine</searchLink>. 2012, Vol. 40 Issue 5, p1085-1097. 13p. 2 Black and White Photographs, 1 Chart, 2 Graphs.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Antioxidant+analysis%22">Antioxidant analysis</searchLink><br /><searchLink fieldCode="DE" term="%22RNA+analysis%22">RNA analysis</searchLink><br /><searchLink fieldCode="DE" term="%22Lipid+analysis%22">Lipid analysis</searchLink><br /><searchLink fieldCode="DE" term="%22Phenol+analysis%22">Phenol analysis</searchLink><br /><searchLink fieldCode="DE" term="%22Animal+experimentation%22">Animal experimentation</searchLink><br /><searchLink fieldCode="DE" term="%22Anti-inflammatory+agents%22">Anti-inflammatory agents</searchLink><br /><searchLink fieldCode="DE" term="%22Bark%22">Bark</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+culture%22">Cell culture</searchLink><br /><searchLink fieldCode="DE" term="%22Macrophages%22">Macrophages</searchLink><br /><searchLink fieldCode="DE" term="%22Medicinal+plants%22">Medicinal plants</searchLink><br /><searchLink fieldCode="DE" term="%22Methanol%22">Methanol</searchLink><br /><searchLink fieldCode="DE" term="%22Nitric+oxide%22">Nitric oxide</searchLink><br /><searchLink fieldCode="DE" term="%22Oxidoreductases%22">Oxidoreductases</searchLink><br /><searchLink fieldCode="DE" term="%22Polymerase+chain+reaction%22">Polymerase chain reaction</searchLink><br /><searchLink fieldCode="DE" term="%22T-test+%28Statistics%29%22">T-test (Statistics)</searchLink><br /><searchLink fieldCode="DE" term="%22Western+immunoblotting%22">Western immunoblotting</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+extracts%22">Plant extracts</searchLink><br /><searchLink fieldCode="DE" term="%22Lipopolysaccharides%22">Lipopolysaccharides</searchLink><br /><searchLink fieldCode="DE" term="%22Descriptive+statistics%22">Descriptive statistics</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Pistacia chinensis has been used for various purposes in China including as an understock for grafting Pistacia vera. However, little attention was given to its health promoting effects. Therefore, in this study, we investigated the effect of Pistacia chinensis methanolic extract (PCME) containing resorcinol class of phenolic lipids on pro-inflammatory mediators and heme oxygenase-1(HO-1) in lipopolysaccharide stimulated RAW264.7 cells. While PCME (2.5-10 μg/ml) inhibited mRNA expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and interleukin (IL)-6, it up-regulated HO-1 expression. Likewise, PCME inhibited iNOS protein expression, but not COX-2, and reduced nitric oxide (NO) release. Moreover, Phosphorylated c-Jun N-terminal Kinase (JNK) was attenuated dose-dependently in PCME pre-treated RAW264.7 cells. In addition, PCME up-regulated HO-1 protein expression was diminished by pre-treatment of PI-3K inhibitor. Furthermore, nuclear factor erythroid 2 related factor 2 (Nrf2) repressor was attenuated time-dependently during PCME treatment. Taken together, our study showed (for the first time) that PCME inhibited NO production and up-regulated HO-1 induction via PI-3K/Akt pathway, suggesting the role of Pistacia chinensis as potential sources of anti-inflammatory and antioxidant natural compounds. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of American Journal of Chinese Medicine is the property of World Scientific Publishing Company and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1142/S0192415X12500802
    Languages:
      – Code: eng
        Text: English
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      Pagination:
        PageCount: 13
        StartPage: 1085
    Subjects:
      – SubjectFull: Antioxidant analysis
        Type: general
      – SubjectFull: RNA analysis
        Type: general
      – SubjectFull: Lipid analysis
        Type: general
      – SubjectFull: Phenol analysis
        Type: general
      – SubjectFull: Animal experimentation
        Type: general
      – SubjectFull: Anti-inflammatory agents
        Type: general
      – SubjectFull: Bark
        Type: general
      – SubjectFull: Cell culture
        Type: general
      – SubjectFull: Macrophages
        Type: general
      – SubjectFull: Medicinal plants
        Type: general
      – SubjectFull: Methanol
        Type: general
      – SubjectFull: Nitric oxide
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      – SubjectFull: Oxidoreductases
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      – SubjectFull: Polymerase chain reaction
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      – SubjectFull: T-test (Statistics)
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      – SubjectFull: Western immunoblotting
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      – SubjectFull: Plant extracts
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      – SubjectFull: Lipopolysaccharides
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      – SubjectFull: Descriptive statistics
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    Titles:
      – TitleFull: Pistacia chinensis Inhibits NO Production and Upregulates HO-1 Induction via PI-3K/Akt Pathway in LPS Stimulated Macrophage Cells.
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              M: 10
              Text: 2012
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