Pistacia chinensis Inhibits NO Production and Upregulates HO-1 Induction via PI-3K/Akt Pathway in LPS Stimulated Macrophage Cells.
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| Title: | Pistacia chinensis Inhibits NO Production and Upregulates HO-1 Induction via PI-3K/Akt Pathway in LPS Stimulated Macrophage Cells. |
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| Authors: | Yayeh, Taddesse, Hong, Mei, Jia, Qi, Lee, Young-Chul, Kim, Hyun-Jin, Hyun, Eujin, Kim, Tae-Wan, Rhee, Man Hee |
| Source: | American Journal of Chinese Medicine. 2012, Vol. 40 Issue 5, p1085-1097. 13p. 2 Black and White Photographs, 1 Chart, 2 Graphs. |
| Subjects: | Antioxidant analysis, RNA analysis, Lipid analysis, Phenol analysis, Animal experimentation, Anti-inflammatory agents, Bark, Cell culture, Macrophages, Medicinal plants, Methanol, Nitric oxide, Oxidoreductases, Polymerase chain reaction, T-test (Statistics), Western immunoblotting, Plant extracts, Lipopolysaccharides, Descriptive statistics |
| Abstract: | Pistacia chinensis has been used for various purposes in China including as an understock for grafting Pistacia vera. However, little attention was given to its health promoting effects. Therefore, in this study, we investigated the effect of Pistacia chinensis methanolic extract (PCME) containing resorcinol class of phenolic lipids on pro-inflammatory mediators and heme oxygenase-1(HO-1) in lipopolysaccharide stimulated RAW264.7 cells. While PCME (2.5-10 μg/ml) inhibited mRNA expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and interleukin (IL)-6, it up-regulated HO-1 expression. Likewise, PCME inhibited iNOS protein expression, but not COX-2, and reduced nitric oxide (NO) release. Moreover, Phosphorylated c-Jun N-terminal Kinase (JNK) was attenuated dose-dependently in PCME pre-treated RAW264.7 cells. In addition, PCME up-regulated HO-1 protein expression was diminished by pre-treatment of PI-3K inhibitor. Furthermore, nuclear factor erythroid 2 related factor 2 (Nrf2) repressor was attenuated time-dependently during PCME treatment. Taken together, our study showed (for the first time) that PCME inhibited NO production and up-regulated HO-1 induction via PI-3K/Akt pathway, suggesting the role of Pistacia chinensis as potential sources of anti-inflammatory and antioxidant natural compounds. [ABSTRACT FROM AUTHOR] |
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| Database: | Psychology and Behavioral Sciences Collection |
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