Contribution of serotonin uptake and degradation to myocardial interstitial serotonin levels during ischaemia-reperfusion in rabbits.
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| Title: | Contribution of serotonin uptake and degradation to myocardial interstitial serotonin levels during ischaemia-reperfusion in rabbits. |
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| Authors: | Sonobe, T., Akiyama, T., Du, C.‐K., Zhan, D.‐Y., Shirai, M. |
| Source: | Acta Physiologica. Feb2013, Vol. 207 Issue 2, p260-268. 9p. 3 Diagrams, 1 Chart, 2 Graphs. |
| Subjects: | Serotonin uptake inhibitors, Cardiomyopathies, Serotonin, Ischemia, Reperfusion, Laboratory rabbits, Monoamine oxidase |
| Abstract: | Aim Although deleterious effects of serotonin (5- HT) have been demonstrated during myocardial ischaemia-reperfusion, little information is available on myocardial interstitial 5- HT kinetics. This study evaluated the contribution of 5- HT reuptake and degradation to myocardial interstitial 5- HT levels during ischaemia-reperfusion. Methods Using microdialysis technique in anaesthetized rabbits, we monitored myocardial interstitial 5- HT levels in the ischaemic region during ischaemia (30 min) followed by reperfusion (60 min) and investigated the effects of local infusion of fluoxetine, a 5- HT uptake inhibitor, and/or pargyline, a monoamine oxidase inhibitor. Results In vehicle control, dialysate 5-HT concentration increased gradually from 16 ± 3 at baseline to 85 ± 18 n m during 20-30 min of ischaemia. Dialysate 5-HT concentration further increased to 236 ± 47 n m at 0-10 min of reperfusion and then began to decline. Averaged 5-HT concentration was 61 ± 11 during ischaemia and 113 ± 13 n m during reperfusion. Fluoxetine elevated dialysate 5-HT level at baseline and at 10-30 min of reperfusion; it increased averaged dialysate 5-HT concentration by approx. 304% during reperfusion compared to control. Pargyline elevated averaged dialysate 5-HT concentration during ischaemia by approx. 243% and that during reperfusion by approx. 250% compared to control. The changes in dialysate 5-HT concentration by fluoxetine + pargyline were similar to those of fluoxetine alone. Conclusion The 5- HT reuptake function plays an important role in the clearance of myocardial interstitial 5- HT during reperfusion. When 5- HT reuptake function is intact, degradation of 5- HT by monoamine oxidase contributes to reduce myocardial interstitial 5- HT level throughout ischaemia-reperfusion. [ABSTRACT FROM AUTHOR] |
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| Database: | Psychology and Behavioral Sciences Collection |
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| Abstract: | Aim Although deleterious effects of serotonin (5- HT) have been demonstrated during myocardial ischaemia-reperfusion, little information is available on myocardial interstitial 5- HT kinetics. This study evaluated the contribution of 5- HT reuptake and degradation to myocardial interstitial 5- HT levels during ischaemia-reperfusion. Methods Using microdialysis technique in anaesthetized rabbits, we monitored myocardial interstitial 5- HT levels in the ischaemic region during ischaemia (30 min) followed by reperfusion (60 min) and investigated the effects of local infusion of fluoxetine, a 5- HT uptake inhibitor, and/or pargyline, a monoamine oxidase inhibitor. Results In vehicle control, dialysate 5-HT concentration increased gradually from 16 ± 3 at baseline to 85 ± 18 n m during 20-30 min of ischaemia. Dialysate 5-HT concentration further increased to 236 ± 47 n m at 0-10 min of reperfusion and then began to decline. Averaged 5-HT concentration was 61 ± 11 during ischaemia and 113 ± 13 n m during reperfusion. Fluoxetine elevated dialysate 5-HT level at baseline and at 10-30 min of reperfusion; it increased averaged dialysate 5-HT concentration by approx. 304% during reperfusion compared to control. Pargyline elevated averaged dialysate 5-HT concentration during ischaemia by approx. 243% and that during reperfusion by approx. 250% compared to control. The changes in dialysate 5-HT concentration by fluoxetine + pargyline were similar to those of fluoxetine alone. Conclusion The 5- HT reuptake function plays an important role in the clearance of myocardial interstitial 5- HT during reperfusion. When 5- HT reuptake function is intact, degradation of 5- HT by monoamine oxidase contributes to reduce myocardial interstitial 5- HT level throughout ischaemia-reperfusion. [ABSTRACT FROM AUTHOR] |
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| ISSN: | 17481708 |
| DOI: | 10.1111/j.1748-1716.2012.02461.x |
