MurJ is the flippase of lipid-linked precursors for peptidoglycan biogenesis.
Saved in:
| Title: | MurJ is the flippase of lipid-linked precursors for peptidoglycan biogenesis. |
|---|---|
| Authors: | Lok-To Sham, Butler, Emily K., Lebar, Matthew D., Kahne, Daniel, Bernhardt, Thomas G., Ruiz, Natividad |
| Source: | Science (pre-March 2025). 7/11/2014, Vol. 345 Issue 6193, p220-222. 3p. |
| Subjects: | Peptidoglycans, Polyisoprene, Escherichia coli, Lipid synthesis, Lipids, Polysaccharides, Lysis |
| Abstract: | Peptidoglycan (PG) is a polysaccharide matrix that protects bacteria from osmotic lysis. Inhibition of its biogenesis is a proven strategy for killing bacteria with antibiotics. The assembly of PG requires disaccharide-pentapeptide building blocks attached to a polyisoprene lipid carrier called lipid II. Although the stages of lipid II synthesis are known, the identity of the essential flippase that translocates it across the cytoplasmic membrane for PG polymerization is unclear. We developed an assay for lipid II flippase activity and used a chemical genetic strategy to rapidly and specifically block flippase function. We combined these approaches to demonstrate that MurJ is the lipid II flippase in Escherichia coli. [ABSTRACT FROM AUTHOR] |
| Copyright of Science (pre-March 2025) is the property of American Association for the Advancement of Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Psychology and Behavioral Sciences Collection |
Be the first to leave a comment!
