The aim of this study was to evaluate the effect of two levels: prostaglandin and glycosaminoglycans in sperm motility. A total of 90 samples were obtained from three adult male Gyr breed (animals from The Biotechnological Center of Caribe, CBC, SENA, Valledupar, Colombia) divided into 6 treatments...
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https://revistas.sena.edu.co/index.php/raaa/article/view/504 |
| Summary: | The aim of this study was to evaluate the effect of two levels: prostaglandin and glycosaminoglycans in sperm motility. A total of 90 samples were obtained from three adult male Gyr breed (animals from The Biotechnological Center of Caribe, CBC, SENA, Valledupar, Colombia) divided into 6 treatments and 5 replicates per treatment. All ejaculated semen was evaluated, divided into 6 aliquots and then, frozen by means of a standard protocol using the commercial diluent, AndroMed®. After freezing, sperm motility was evaluated by means of computer-assisted sperm analysis (CASA). The control treatment and the cloprostenol treatment (0.1 mL) showed statistically higher values (P = 0.05) for most of the evaluated seminal parameters: motility (68.71 ± 26.3% and 12.9 ± 60.80 %, respectively); and progressiveness (18.75 ± 17.01 ± 11.3% and 6.9%, respectively). Hyaluronic acid treatment (0.1 uL) showed the best values in the parameters of curvilinear (VCL) and average path velocity (VAP), and the mean amplitude of lateral sperm head (ALH) displacement. For straight-line velocity (VSL), linearity index (LIN), straightness index (STR), wobble (WOB) and beat cross frequency (BCF), there were no statistical differences (p =0.05). In conclusion, the data obtained in the study reflects sperm damage due to cryopreservation of bovine sperm at the CBC. |
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