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The Vitotox and ToxTracker assays: A two-test combination for quick and reliable assessment of genotoxic hazards.

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Title: The Vitotox and ToxTracker assays: A two-test combination for quick and reliable assessment of genotoxic hazards.
Authors: Ates, Gamze1 gamze.ates@vub.ac.be, Favyts, Dorien1, Hendriks, Giel2, Derr, Remco2, Mertens, Birgit3, Verschaeve, Luc3, Rogiers, Vera1, Y. Doktorova, Tatyana3
Source: Mutation Research - Genetic Toxicology & Environmental Mutagenesis. Nov2016, Vol. 810, p13-21. 9p.
Subject Terms: *Genetic toxicology, *Drug additives, Vinblastine, Biliverdin, Chromosome abnormalities
Abstract: To ensure safety for humans, it is essential to characterize the genotoxic potential of new chemical entities, such as pharmaceutical and cosmetic substances. In a first tier, a battery of in vitro tests is recommended by international regulatory agencies. However, these tests suffer from inadequate specificity: compounds may be wrongly categorized as genotoxic, resulting in unnecessary, time-consuming, and expensive in vivo follow-up testing. In the last decade, novel assays (notably, reporter-based assays) have been developed in an attempt to overcome these drawbacks. Here, we have investigated the performance of two in vitro reporter-based assays, Vitotox and ToxTracker. A set of reference compounds was selected to span a variety of mechanisms of genotoxic action and applicability domains ( e.g ., pharmaceutical and cosmetic ingredients). Combining the performance of the two assays, we achieved 93% sensitivity and 79% specificity for prediction of gentoxicity for this set of compounds. Both assays permit quick high-throughput analysis of drug candidates, while requiring only small quantities of the test substances. Our study shows that these two assays, when combined, can be a reliable method for assessment of genotoxicity hazard. [ABSTRACT FROM AUTHOR]
Copyright of Mutation Research - Genetic Toxicology & Environmental Mutagenesis is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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Header DbId: 8gh
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An: 119001383
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PubType: Academic Journal
PubTypeId: academicJournal
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  Data: The Vitotox and ToxTracker assays: A two-test combination for quick and reliable assessment of genotoxic hazards.
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  Data: <searchLink fieldCode="AR" term="%22Ates%2C+Gamze%22">Ates, Gamze</searchLink><relatesTo>1</relatesTo><i> gamze.ates@vub.ac.be</i><br /><searchLink fieldCode="AR" term="%22Favyts%2C+Dorien%22">Favyts, Dorien</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Hendriks%2C+Giel%22">Hendriks, Giel</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Derr%2C+Remco%22">Derr, Remco</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Mertens%2C+Birgit%22">Mertens, Birgit</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Verschaeve%2C+Luc%22">Verschaeve, Luc</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Rogiers%2C+Vera%22">Rogiers, Vera</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Y%2E+Doktorova%2C+Tatyana%22">Y. Doktorova, Tatyana</searchLink><relatesTo>3</relatesTo>
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  Data: <searchLink fieldCode="JN" term="%22Mutation+Research+-+Genetic+Toxicology+%26+Environmental+Mutagenesis%22">Mutation Research - Genetic Toxicology & Environmental Mutagenesis</searchLink>. Nov2016, Vol. 810, p13-21. 9p.
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  Data: *<searchLink fieldCode="DE" term="%22Genetic+toxicology%22">Genetic toxicology</searchLink><br />*<searchLink fieldCode="DE" term="%22Drug+additives%22">Drug additives</searchLink><br /><searchLink fieldCode="DE" term="%22Vinblastine%22">Vinblastine</searchLink><br /><searchLink fieldCode="DE" term="%22Biliverdin%22">Biliverdin</searchLink><br /><searchLink fieldCode="DE" term="%22Chromosome+abnormalities%22">Chromosome abnormalities</searchLink>
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  Data: To ensure safety for humans, it is essential to characterize the genotoxic potential of new chemical entities, such as pharmaceutical and cosmetic substances. In a first tier, a battery of in vitro tests is recommended by international regulatory agencies. However, these tests suffer from inadequate specificity: compounds may be wrongly categorized as genotoxic, resulting in unnecessary, time-consuming, and expensive in vivo follow-up testing. In the last decade, novel assays (notably, reporter-based assays) have been developed in an attempt to overcome these drawbacks. Here, we have investigated the performance of two in vitro reporter-based assays, Vitotox and ToxTracker. A set of reference compounds was selected to span a variety of mechanisms of genotoxic action and applicability domains ( e.g ., pharmaceutical and cosmetic ingredients). Combining the performance of the two assays, we achieved 93% sensitivity and 79% specificity for prediction of gentoxicity for this set of compounds. Both assays permit quick high-throughput analysis of drug candidates, while requiring only small quantities of the test substances. Our study shows that these two assays, when combined, can be a reliable method for assessment of genotoxicity hazard. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
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  Data: <i>Copyright of Mutation Research - Genetic Toxicology & Environmental Mutagenesis is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1016/j.mrgentox.2016.09.005
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        Text: English
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        Type: general
      – SubjectFull: Drug additives
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      – SubjectFull: Vinblastine
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      – SubjectFull: Biliverdin
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      – SubjectFull: Chromosome abnormalities
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      – TitleFull: The Vitotox and ToxTracker assays: A two-test combination for quick and reliable assessment of genotoxic hazards.
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              Text: Nov2016
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