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Combined Effects of in Utero and Adolescent Tobacco Smoke Exposure on Lung Function in C57BI/6J Mice.

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Title: Combined Effects of in Utero and Adolescent Tobacco Smoke Exposure on Lung Function in C57BI/6J Mice.
Authors: Drummond, David1, Baravalle-Einaudi, Melissa1, Lezmi, Guillaume1, Vibhushan, Shamila1, Franco-Montoya, Marie-Laure1, Boczkowski, Jorge1, Hadchouel, Alice1,2,3, Delacourt, Christophe1,2,3
Source: Environmental Health Perspectives. Mar2017, Vol. 125 Issue 3, p392-399. 8p. 1 Diagram, 1 Chart, 3 Graphs.
Subject Terms: *Animal experimentation, *Smoking, *Environmental exposure, Lung physiology, Protein analysis, Obstructive lung diseases, Lung analysis, Biological models, Bronchi, Cellular aging, Collagen, Disease susceptibility, Gene expression, Lungs, Research methodology, Mice, Probability theory, Research funding, Stains & staining (Microscopy), Statistics, Tissue culture, Tobacco, Data analysis, Data analysis software, Descriptive statistics, Prenatal exposure delayed effects, Mann Whitney U Test, Kruskal-Wallis Test, Disease risk factors
Abstract: BACKGROUND: Fetal determinants of airway function, such as in utero exposure to maternal cigarette smoke (CS), may create a predisposition to adult airflow obstruction and chronic obstructive pulmonary disease (COPD) in adulthood. It has been suggested that active smoking in adolescence and preexisting airflow obstruction have synergistic deleterious effects. OBJECTIVE: We used a mouse model to investigate whether there is a synergistic effect of exposure to CS in utero and during adolescence on lung function. METHODS: Female C57B1/6J mice were exposed to CS or to filtered room air during pregnancy. Exposure to CS began 2 weeks before mating and continued until delivery. After birth, the pups were not exposed to CS until day 21 (D21). Between D21 and D49, corresponding to "adolescence," litters were randomized for an additional 4 weeks of exposure to CS. Lung morphometry, lung mechanics, and the expression of genes involved in senescence were evaluated in different subsets of mice on D21 and D49. RESULTS: In utero exposure to CS induced significant lung function impairment by D21. CS exposure between D21 and D49 induced significant functional impairment only in mice exposed to CS prenatally. On D49, no difference was observed between subgroups in terms of lung p53, pl6, p21, and Bax mRNA levels. CONCLUSIONS: Our findings suggest that prenatal and adolescent CS exposure have a synergistic effect on lung function in mice. The combined effect did not appear to be a consequence of early pulmonary senescence. CITATION: Drummond D, Baravalle-Einaudi M, Lezmi G, Vibhushan S, Franco-Montoya ML, Hadchouel A, Boczkowski J, Delacourt C. 2017. Combined effects of in utero and adolescent tobacco smoke exposure on lung function in C57B1/6J mice. Environ Health Perspect 125:392-399; http://dx.doi.org/10.1289/EHP54 [ABSTRACT FROM AUTHOR]
Copyright of Environmental Health Perspectives is the property of National Institute of Environmental Health Sciences and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Combined Effects of in Utero and Adolescent Tobacco Smoke Exposure on Lung Function in C57BI/6J Mice.
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  Data: <searchLink fieldCode="JN" term="%22Environmental+Health+Perspectives%22">Environmental Health Perspectives</searchLink>. Mar2017, Vol. 125 Issue 3, p392-399. 8p. 1 Diagram, 1 Chart, 3 Graphs.
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  Data: *<searchLink fieldCode="DE" term="%22Animal+experimentation%22">Animal experimentation</searchLink><br />*<searchLink fieldCode="DE" term="%22Smoking%22">Smoking</searchLink><br />*<searchLink fieldCode="DE" term="%22Environmental+exposure%22">Environmental exposure</searchLink><br /><searchLink fieldCode="DE" term="%22Lung+physiology%22">Lung physiology</searchLink><br /><searchLink fieldCode="DE" term="%22Protein+analysis%22">Protein analysis</searchLink><br /><searchLink fieldCode="DE" term="%22Obstructive+lung+diseases%22">Obstructive lung diseases</searchLink><br /><searchLink fieldCode="DE" term="%22Lung+analysis%22">Lung analysis</searchLink><br /><searchLink fieldCode="DE" term="%22Biological+models%22">Biological models</searchLink><br /><searchLink fieldCode="DE" term="%22Bronchi%22">Bronchi</searchLink><br /><searchLink fieldCode="DE" term="%22Cellular+aging%22">Cellular aging</searchLink><br /><searchLink fieldCode="DE" term="%22Collagen%22">Collagen</searchLink><br /><searchLink fieldCode="DE" term="%22Disease+susceptibility%22">Disease susceptibility</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+expression%22">Gene expression</searchLink><br /><searchLink fieldCode="DE" term="%22Lungs%22">Lungs</searchLink><br /><searchLink fieldCode="DE" term="%22Research+methodology%22">Research methodology</searchLink><br /><searchLink fieldCode="DE" term="%22Mice%22">Mice</searchLink><br /><searchLink fieldCode="DE" term="%22Probability+theory%22">Probability theory</searchLink><br /><searchLink fieldCode="DE" term="%22Research+funding%22">Research funding</searchLink><br /><searchLink fieldCode="DE" term="%22Stains+%26+staining+%28Microscopy%29%22">Stains & staining (Microscopy)</searchLink><br /><searchLink fieldCode="DE" term="%22Statistics%22">Statistics</searchLink><br /><searchLink fieldCode="DE" term="%22Tissue+culture%22">Tissue culture</searchLink><br /><searchLink fieldCode="DE" term="%22Tobacco%22">Tobacco</searchLink><br /><searchLink fieldCode="DE" term="%22Data+analysis%22">Data analysis</searchLink><br /><searchLink fieldCode="DE" term="%22Data+analysis+software%22">Data analysis software</searchLink><br /><searchLink fieldCode="DE" term="%22Descriptive+statistics%22">Descriptive statistics</searchLink><br /><searchLink fieldCode="DE" term="%22Prenatal+exposure+delayed+effects%22">Prenatal exposure delayed effects</searchLink><br /><searchLink fieldCode="DE" term="%22Mann+Whitney+U+Test%22">Mann Whitney U Test</searchLink><br /><searchLink fieldCode="DE" term="%22Kruskal-Wallis+Test%22">Kruskal-Wallis Test</searchLink><br /><searchLink fieldCode="DE" term="%22Disease+risk+factors%22">Disease risk factors</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: BACKGROUND: Fetal determinants of airway function, such as in utero exposure to maternal cigarette smoke (CS), may create a predisposition to adult airflow obstruction and chronic obstructive pulmonary disease (COPD) in adulthood. It has been suggested that active smoking in adolescence and preexisting airflow obstruction have synergistic deleterious effects. OBJECTIVE: We used a mouse model to investigate whether there is a synergistic effect of exposure to CS in utero and during adolescence on lung function. METHODS: Female C57B1/6J mice were exposed to CS or to filtered room air during pregnancy. Exposure to CS began 2 weeks before mating and continued until delivery. After birth, the pups were not exposed to CS until day 21 (D21). Between D21 and D49, corresponding to "adolescence," litters were randomized for an additional 4 weeks of exposure to CS. Lung morphometry, lung mechanics, and the expression of genes involved in senescence were evaluated in different subsets of mice on D21 and D49. RESULTS: In utero exposure to CS induced significant lung function impairment by D21. CS exposure between D21 and D49 induced significant functional impairment only in mice exposed to CS prenatally. On D49, no difference was observed between subgroups in terms of lung p53, pl6, p21, and Bax mRNA levels. CONCLUSIONS: Our findings suggest that prenatal and adolescent CS exposure have a synergistic effect on lung function in mice. The combined effect did not appear to be a consequence of early pulmonary senescence. CITATION: Drummond D, Baravalle-Einaudi M, Lezmi G, Vibhushan S, Franco-Montoya ML, Hadchouel A, Boczkowski J, Delacourt C. 2017. Combined effects of in utero and adolescent tobacco smoke exposure on lung function in C57B1/6J mice. Environ Health Perspect 125:392-399; http://dx.doi.org/10.1289/EHP54 [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
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  Data: <i>Copyright of Environmental Health Perspectives is the property of National Institute of Environmental Health Sciences and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1289/EHP54
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        Text: English
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    Subjects:
      – SubjectFull: Animal experimentation
        Type: general
      – SubjectFull: Smoking
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      – SubjectFull: Environmental exposure
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      – SubjectFull: Lung physiology
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