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PCR BASED MOLECULAR CHARACTERIZATION OF CITRUS BACTERIAL CANKER FROM GRAPEFRUIT (CITRUS PARADISI).

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Title: PCR BASED MOLECULAR CHARACTERIZATION OF CITRUS BACTERIAL CANKER FROM GRAPEFRUIT (CITRUS PARADISI).
Authors: Tazeen, Syeda khola1 kholatazeen02@gmail.com, Akbar, Khalid Farooq1, Khan, Arif Muhammad2, Masood, Atifa1, Fayyaz, Amna3, Khan, Umair A.1, Arooj, Sumaira4
Source: Fresenius Environmental Bulletin. 2019, Vol. 28 Issue 5, p3724-3728. 5p.
Subject Terms: Citrus canker, Citrus, Grapefruit, Xanthomonas campestris, Polymerase chain reaction, Pathogenic bacteria, Citrus varieties
Geographic Terms: India, United States, Japan
Company/Entity: Canadian Broadcasting Corp.
Abstract: Citrus bacterial canker (CBC) occurs due to Xanthomonas axonopodis pv. Citri (Xac) is a pathogenic bacteria. It was first reported in Japan, USA and many areas of India in 19th century. Synchronized by the spread of the causative agent the diversity of labelled pathogen continues to spread with new strains appearing in many other regions of the world. However, its diagnosis is quite difficult due to its symptoms similarity with other bacterial strains. This piece of study was designed to identify Xanthomonas axonopodis using polymerase chain reaction (PCR). PCR is more accurate, precise and fast method which is used for detection and quantification of many bacterial species. Two primers Xac01 and Xac02 were used for PCR based CBC detection. These primers were based on rpf gene region which give very specific results in Xac identification and can separate two much similar bacterial strains. There is another Primer pair designed on the pthA gene which act as universal primer and usually used for detection of all varieties of citrus canker bacteria. Leaves sample from different infected Citrus paradisi plants were collected for further analysis. Results confirmed that three samples among five have CBC. It is concluded that using PCR we can detect citrus canker disease more specifically which will help in discovering new eradication methods for its control. [ABSTRACT FROM AUTHOR]
Copyright of Fresenius Environmental Bulletin is the property of PRT-Parlar Research & Technology and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: PCR BASED MOLECULAR CHARACTERIZATION OF CITRUS BACTERIAL CANKER FROM GRAPEFRUIT (CITRUS PARADISI).
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  Data: <searchLink fieldCode="AR" term="%22Tazeen%2C+Syeda+khola%22">Tazeen, Syeda khola</searchLink><relatesTo>1</relatesTo><i> kholatazeen02@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Akbar%2C+Khalid+Farooq%22">Akbar, Khalid Farooq</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Khan%2C+Arif+Muhammad%22">Khan, Arif Muhammad</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Masood%2C+Atifa%22">Masood, Atifa</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Fayyaz%2C+Amna%22">Fayyaz, Amna</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Khan%2C+Umair+A%2E%22">Khan, Umair A.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Arooj%2C+Sumaira%22">Arooj, Sumaira</searchLink><relatesTo>4</relatesTo>
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  Data: <searchLink fieldCode="JN" term="%22Fresenius+Environmental+Bulletin%22">Fresenius Environmental Bulletin</searchLink>. 2019, Vol. 28 Issue 5, p3724-3728. 5p.
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  Data: <searchLink fieldCode="DE" term="%22Citrus+canker%22">Citrus canker</searchLink><br /><searchLink fieldCode="DE" term="%22Citrus%22">Citrus</searchLink><br /><searchLink fieldCode="DE" term="%22Grapefruit%22">Grapefruit</searchLink><br /><searchLink fieldCode="DE" term="%22Xanthomonas+campestris%22">Xanthomonas campestris</searchLink><br /><searchLink fieldCode="DE" term="%22Polymerase+chain+reaction%22">Polymerase chain reaction</searchLink><br /><searchLink fieldCode="DE" term="%22Pathogenic+bacteria%22">Pathogenic bacteria</searchLink><br /><searchLink fieldCode="DE" term="%22Citrus+varieties%22">Citrus varieties</searchLink>
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  Label: Geographic Terms
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  Data: <searchLink fieldCode="DE" term="%22India%22">India</searchLink><br /><searchLink fieldCode="DE" term="%22United+States%22">United States</searchLink><br /><searchLink fieldCode="DE" term="%22Japan%22">Japan</searchLink>
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  Data: <searchLink fieldCode="DE" term="%22Canadian+Broadcasting+Corp%2E%22">Canadian Broadcasting Corp.</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Citrus bacterial canker (CBC) occurs due to Xanthomonas axonopodis pv. Citri (Xac) is a pathogenic bacteria. It was first reported in Japan, USA and many areas of India in 19th century. Synchronized by the spread of the causative agent the diversity of labelled pathogen continues to spread with new strains appearing in many other regions of the world. However, its diagnosis is quite difficult due to its symptoms similarity with other bacterial strains. This piece of study was designed to identify Xanthomonas axonopodis using polymerase chain reaction (PCR). PCR is more accurate, precise and fast method which is used for detection and quantification of many bacterial species. Two primers Xac01 and Xac02 were used for PCR based CBC detection. These primers were based on rpf gene region which give very specific results in Xac identification and can separate two much similar bacterial strains. There is another Primer pair designed on the pthA gene which act as universal primer and usually used for detection of all varieties of citrus canker bacteria. Leaves sample from different infected Citrus paradisi plants were collected for further analysis. Results confirmed that three samples among five have CBC. It is concluded that using PCR we can detect citrus canker disease more specifically which will help in discovering new eradication methods for its control. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Fresenius Environmental Bulletin is the property of PRT-Parlar Research & Technology and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Code: eng
        Text: English
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      Pagination:
        PageCount: 5
        StartPage: 3724
    Subjects:
      – SubjectFull: Citrus canker
        Type: general
      – SubjectFull: Citrus
        Type: general
      – SubjectFull: Grapefruit
        Type: general
      – SubjectFull: Xanthomonas campestris
        Type: general
      – SubjectFull: Polymerase chain reaction
        Type: general
      – SubjectFull: Pathogenic bacteria
        Type: general
      – SubjectFull: Citrus varieties
        Type: general
      – SubjectFull: India
        Type: general
      – SubjectFull: United States
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      – SubjectFull: Japan
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      – SubjectFull: Canadian Broadcasting Corp.
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      – TitleFull: PCR BASED MOLECULAR CHARACTERIZATION OF CITRUS BACTERIAL CANKER FROM GRAPEFRUIT (CITRUS PARADISI).
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            NameFull: Tazeen, Syeda khola
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            NameFull: Akbar, Khalid Farooq
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            NameFull: Khan, Arif Muhammad
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            NameFull: Masood, Atifa
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            NameFull: Fayyaz, Amna
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              M: 05
              Text: 2019
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