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A Shear horizontal surface acoustic wave biosensor for a rapid and specific detection of d-serine.

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Title: A Shear horizontal surface acoustic wave biosensor for a rapid and specific detection of d-serine.
Authors: Di Pietrantonio, F.1, Benetti, M.1, Cannatà, D.1, Verona, E.2, Girasole, M.3, Fosca, M.3, Dinarelli, S.3, Staiano, M.4, Marzullo, V.M.5, Capo, A.4, Varriale, A.4, D’Auria, S.4 sabato.dauria@cnr.it
Source: Sensors & Actuators B: Chemical. Apr2016, Vol. 226, p1-6. 6p.
Subjects: Shear (Mechanics), Surface acoustic wave sensors, Serine, Amino acids, Cellular signal transduction, Aspartates
Abstract: d -serine is an amino acid that has an important role in the regulation of N-methyl- d -aspartate (NMDA) signalling in the central nervous system. In fact, it acts as an endogenous co-agonist molecule for the NMDA receptor activation. Since an abnormal body level of d -amino acids is usually associated to different neurological diseases, it appears evident that it is of high interest to develop a fast and specific biosensor for the determination of d -serine. In this work, we report on the design and development of a surface acoustic wave (SAW) biosensor to detect the level of d -serine. The SAW-biosensor is based on the utilization of an inactivated form of the d -serine dehydratase isolated from Saccharomyces cerevisiae , a biomolecule that is able to specifically bind d -serine. In particular, we produced a PLP-reduced d -serine dehydratase biomolecule that was still able to bind d -serine and not to transform it into the product. The binding event between the “inactivated” d -serine dehydratase and d -serine was monitored by SAW delay-lines. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
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Abstract:d -serine is an amino acid that has an important role in the regulation of N-methyl- d -aspartate (NMDA) signalling in the central nervous system. In fact, it acts as an endogenous co-agonist molecule for the NMDA receptor activation. Since an abnormal body level of d -amino acids is usually associated to different neurological diseases, it appears evident that it is of high interest to develop a fast and specific biosensor for the determination of d -serine. In this work, we report on the design and development of a surface acoustic wave (SAW) biosensor to detect the level of d -serine. The SAW-biosensor is based on the utilization of an inactivated form of the d -serine dehydratase isolated from Saccharomyces cerevisiae , a biomolecule that is able to specifically bind d -serine. In particular, we produced a PLP-reduced d -serine dehydratase biomolecule that was still able to bind d -serine and not to transform it into the product. The binding event between the “inactivated” d -serine dehydratase and d -serine was monitored by SAW delay-lines. [ABSTRACT FROM AUTHOR]
ISSN:09254005
DOI:10.1016/j.snb.2015.11.099