View in EDS

Effect of cobalt and chromium ions on bcl-2, bax, caspase-3, and caspase-8 expression in human U937 macrophages

Saved in:
Bibliographic Details
Title: Effect of cobalt and chromium ions on bcl-2, bax, caspase-3, and caspase-8 expression in human U937 macrophages
Authors: Petit, Alain1, Mwale, Fackson1, Zukor, David J.1, Catelas, Isabelle1, Antoniou, John1, Huk, Olga L. ohuk@ldi.jgh.mcgill.ca
Source: Biomaterials. May2004, Vol. 25 Issue 11, p2013. 6p.
Subjects: Proteins, Metal ions, Chromium, Apoptosis
Abstract: The bcl-2 and caspase families of proteins play a central role in the modulation of apoptosis. The purpose of this study was to analyze the effect of Co2+ and Cr3+ ions on the expression of bcl-2, bax, caspase-3 and caspase-8 to better understand the mechanisms leading to ion-induced apoptosis in macrophages. U937 human macrophages were exposed to Co2+ and Cr3+ ions. The expression of proteins was measured by Western blot while caspase activities were measured by colorimetric assay. Results show that Co2+ ions inhibited bcl-2 expression with significant effect (p<0.05) after 16 h and a maximal 52% inhibitory effect after 24 h. Co2+ stimulated bax expression with a significant stimulation (p<0.05) after 8 h and a maximal 1.75-fold increase after 16 h. Co2+ also stimulated the expression of the active fragment of caspase-3 as well as caspase-3 activity maximal increase after 24 h. Co2+ ions had no effect on caspase-8 expression or activity.Cr3+ ions inhibited bcl-2 expression with significant effect (p<0.05) after 16 h and a maximal 43% inhibitory effect after 24 h. Cr3+ stimulated bax expression with significant stimulation (p<0.01) after 8 h and a maximal 2.25-fold increase after 24 h. Cr3+ ions also stimulated the expression of the active fragments of caspase-3 and -8, as well as the activities of both proteases. The effect of Cr3+ ions on the expression of both caspase active fragments was maximal after 16 h incubation. In conclusion, our results suggest that the modulation of the expression of proteins from the bcl-2 and the caspase families of proteins are implicated in the induction of macrophage apoptosis by Co2+ and Cr3+ ions. [Copyright &y& Elsevier]
Copyright of Biomaterials is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
Description
Abstract:The bcl-2 and caspase families of proteins play a central role in the modulation of apoptosis. The purpose of this study was to analyze the effect of Co2+ and Cr3+ ions on the expression of bcl-2, bax, caspase-3 and caspase-8 to better understand the mechanisms leading to ion-induced apoptosis in macrophages. U937 human macrophages were exposed to Co2+ and Cr3+ ions. The expression of proteins was measured by Western blot while caspase activities were measured by colorimetric assay. Results show that Co2+ ions inhibited bcl-2 expression with significant effect (<f>p<0.05</f>) after 16 h and a maximal 52% inhibitory effect after 24 h. Co2+ stimulated bax expression with a significant stimulation (<f>p<0.05</f>) after 8 h and a maximal 1.75-fold increase after 16 h. Co2+ also stimulated the expression of the active fragment of caspase-3 as well as caspase-3 activity maximal increase after 24 h. Co2+ ions had no effect on caspase-8 expression or activity.Cr3+ ions inhibited bcl-2 expression with significant effect (<f>p<0.05</f>) after 16 h and a maximal 43% inhibitory effect after 24 h. Cr3+ stimulated bax expression with significant stimulation (<f>p<0.01</f>) after 8 h and a maximal 2.25-fold increase after 24 h. Cr3+ ions also stimulated the expression of the active fragments of caspase-3 and -8, as well as the activities of both proteases. The effect of Cr3+ ions on the expression of both caspase active fragments was maximal after 16 h incubation. In conclusion, our results suggest that the modulation of the expression of proteins from the bcl-2 and the caspase families of proteins are implicated in the induction of macrophage apoptosis by Co2+ and Cr3+ ions. [Copyright &y& Elsevier]
ISSN:01429612
DOI:10.1016/j.biomaterials.2003.08.040