When size matters: differences in demineralized bone matrix particles affect collagen structure, mesenchymal stem cell behavior, and osteogenic potential.
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| Title: | When size matters: differences in demineralized bone matrix particles affect collagen structure, mesenchymal stem cell behavior, and osteogenic potential. |
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| Authors: | Dozza, B.1,2 barbara.dozza@ior.it, Lesci, I. G.3, Duchi, S.1, Della Bella, E.4,5, Martini, L.4, Salamanna, F.4, Falconi, M.6, Cinotti, S.7, Fini, M.4, Lucarelli, E.1, Donati, D.1,2 |
| Source: | Journal of Biomedical Materials Research, Part A. Apr2017, Vol. 105 Issue 4, p1019-1033. 15p. |
| Abstract: | Demineralized bone matrix (DBM) is a natural, collagen-based, osteoinductive biomaterial. Nevertheless, there are conflicting reports on the efficacy of this product. The purpose of this study was to evaluate whether DBM collagen structure is affected by particle size and can influence DBM cytocompatibility and osteoinductivity. Sheep cortical bone was ground and particles were divided in three fractions with different sizes, defined as large (L, 1-2 mm), medium (M, 0.5-1 mm), and small (S, <0.5 mm). After demineralization, the chemical-physical analysis clearly showed a particle size-dependent alteration in collagen structure, with DBM-M being altered but not as much as DBM-S. DBM-M displayed a preferable trend in almost all biological characteristics tested, although all DBM particles revealed an optimal cytocompatibility. Subcutaneous implantation of DBM particles into immunocompromised mice resulted in bone induction only for DBM-M. When sheep MSC were seeded onto particles before implantation, all DBM particles were able to induce new bone formation with the best incidence for DBM-M and DBM-S. In conclusion, the collagen alteration in DBM-M is likely the best condition to promote bone induction in vivo. Furthermore, the choice of 0.5-1 mm particles may enable to obtain more efficient and consistent results among different research groups in bone tissue-engineering applications. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1019-1033, 2017. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Biomedical Materials Research, Part A is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 121443119 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1002/jbm.a.35975 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 15 StartPage: 1019 Titles: – TitleFull: When size matters: differences in demineralized bone matrix particles affect collagen structure, mesenchymal stem cell behavior, and osteogenic potential. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Dozza, B. – PersonEntity: Name: NameFull: Lesci, I. G. – PersonEntity: Name: NameFull: Duchi, S. – PersonEntity: Name: NameFull: Della Bella, E. – PersonEntity: Name: NameFull: Martini, L. – PersonEntity: Name: NameFull: Salamanna, F. – PersonEntity: Name: NameFull: Falconi, M. – PersonEntity: Name: NameFull: Cinotti, S. – PersonEntity: Name: NameFull: Fini, M. – PersonEntity: Name: NameFull: Lucarelli, E. – PersonEntity: Name: NameFull: Donati, D. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 04 Text: Apr2017 Type: published Y: 2017 Identifiers: – Type: issn-print Value: 15493296 Numbering: – Type: volume Value: 105 – Type: issue Value: 4 Titles: – TitleFull: Journal of Biomedical Materials Research, Part A Type: main |
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