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A beamline‐compatible STED microscope for combined visible‐light and X‐ray studies of biological matter.

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Title: A beamline‐compatible STED microscope for combined visible‐light and X‐ray studies of biological matter.
Authors: Bernhardt, Marten1 (AUTHOR), Nicolas, Jan-David1 (AUTHOR), Osterhoff, Markus1 (AUTHOR), Mittelstädt, Haugen2 (AUTHOR), Reuss, Matthias2 (AUTHOR), Harke, Benjamin2 (AUTHOR), Wittmeier, Andrew1 (AUTHOR), Sprung, Michael3 (AUTHOR), Köster, Sarah1 (AUTHOR), Salditt, Tim1 (AUTHOR) tsaldit@gwdg.de
Source: Journal of Synchrotron Radiation. Jul2019, Vol. 26 Issue 4, p1144-1151. 8p.
Subjects: Holography, Small-angle X-ray scattering, X-rays, Stimulated emission, Microscopes, Storage rings
Geographic Terms: Petra (Extinct city), Göttingen (Germany)
Abstract: A dedicated stimulated emission depletion (STED) microscope had been designed and implemented into the Göttingen Instrument for Nano‐Imaging with X‐rays (GINIX) at the synchrotron beamline P10 of the PETRA III storage ring (DESY, Hamburg). The microscope was installed on the same optical table used for X‐ray holography and scanning small‐angle X‐ray scattering (SAXS). Scanning SAXS was implemented with the Kirkpatrick–Baez (KB) nano‐focusing optics of GINIX, while X‐ray holography used a combined KB and X‐ray waveguide optical system for full‐field projection recordings at a defocus position of the object. The STED optical axis was aligned (anti‐)parallel to the focused synchrotron beam and was laterally displaced from the KB focus. This close proximity between the STED and the X‐ray probe enabled in situ combined recordings on the same biological cell, tissue or any other biomolecular sample, using the same environment and mounting. Here, the instrumentation and experimental details of this correlative microscopy approach are described, as first published in our preceding work [Bernhardt et al. (2018), Nat. Commun.9, 3641], and the capabilities of correlative STED microscopy, X‐ray holography and scanning SAXS are illustrated by presenting additional datasets on cardiac tissue cells with labeled actin cytoskeleton. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Synchrotron Radiation is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: A beamline‐compatible STED microscope for combined visible‐light and X‐ray studies of biological matter.
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  Data: <searchLink fieldCode="AR" term="%22Bernhardt%2C+Marten%22">Bernhardt, Marten</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Nicolas%2C+Jan-David%22">Nicolas, Jan-David</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Osterhoff%2C+Markus%22">Osterhoff, Markus</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Mittelstädt%2C+Haugen%22">Mittelstädt, Haugen</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Reuss%2C+Matthias%22">Reuss, Matthias</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Harke%2C+Benjamin%22">Harke, Benjamin</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wittmeier%2C+Andrew%22">Wittmeier, Andrew</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Sprung%2C+Michael%22">Sprung, Michael</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Köster%2C+Sarah%22">Köster, Sarah</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Salditt%2C+Tim%22">Salditt, Tim</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> tsaldit@gwdg.de</i>
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  Data: A dedicated stimulated emission depletion (STED) microscope had been designed and implemented into the Göttingen Instrument for Nano‐Imaging with X‐rays (GINIX) at the synchrotron beamline P10 of the PETRA III storage ring (DESY, Hamburg). The microscope was installed on the same optical table used for X‐ray holography and scanning small‐angle X‐ray scattering (SAXS). Scanning SAXS was implemented with the Kirkpatrick–Baez (KB) nano‐focusing optics of GINIX, while X‐ray holography used a combined KB and X‐ray waveguide optical system for full‐field projection recordings at a defocus position of the object. The STED optical axis was aligned (anti‐)parallel to the focused synchrotron beam and was laterally displaced from the KB focus. This close proximity between the STED and the X‐ray probe enabled in situ combined recordings on the same biological cell, tissue or any other biomolecular sample, using the same environment and mounting. Here, the instrumentation and experimental details of this correlative microscopy approach are described, as first published in our preceding work [Bernhardt et al. (2018), Nat. Commun.9, 3641], and the capabilities of correlative STED microscopy, X‐ray holography and scanning SAXS are illustrated by presenting additional datasets on cardiac tissue cells with labeled actin cytoskeleton. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
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  Data: <i>Copyright of Journal of Synchrotron Radiation is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1107/S1600577519004089
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      – Code: eng
        Text: English
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        PageCount: 8
        StartPage: 1144
    Subjects:
      – SubjectFull: Holography
        Type: general
      – SubjectFull: Small-angle X-ray scattering
        Type: general
      – SubjectFull: X-rays
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      – SubjectFull: Stimulated emission
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      – SubjectFull: Storage rings
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      – SubjectFull: Petra (Extinct city)
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      – SubjectFull: Göttingen (Germany)
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      – TitleFull: A beamline‐compatible STED microscope for combined visible‐light and X‐ray studies of biological matter.
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              Text: Jul2019
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              Y: 2019
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