Recent advances in the mass spectrometric analysis of glycosphingolipidome – A review.
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| Title: | Recent advances in the mass spectrometric analysis of glycosphingolipidome – A review. |
|---|---|
| Authors: | Barrientos, Rodell C.1,2 (AUTHOR), Zhang, Qibin1,2 (AUTHOR) q_zhang2@uncg.edu |
| Source: | Analytica Chimica Acta. Oct2020, Vol. 1132, p134-155. 22p. |
| Subjects: | Tandem mass spectrometry, Ion mobility spectroscopy, Mass spectrometry, Biological nomenclature, Glycosphingolipids, Isomers |
| Abstract: | Aberrant expression of glycosphingolipids has been implicated in a myriad of diseases, but our understanding of the strucural diversity, spatial distribution, and biological function of this class of biomolecules remains limited. These challenges partly stem from a lack of sensitive tools that can detect, identify, and quantify glycosphingolipids at the molecular level. Mass spectrometry has emerged as a powerful tool poised to address most of these challenges. Here, we review the recent developments in analytical glycosphingolipidomics with an emphasis on sample preparation, mass spectrometry and tandem mass spectrometry-based structural characterization, label-free and labeling-based quantification. We also discuss the nomenclature of glycosphingolipids, and emerging technologies like ion mobility spectrometry in differentiation of glycosphingolipid isomers. The intrinsic advantages and shortcomings of each method are carefully critiqued in line with an individual's research goals. Finally, future perspectives on analytical sphingolipidomics are stated, including a need for novel and more sensive methods in isomer separation, low abundance species detection, and profiling the spatial distribution of glycosphingolipid molecular species in cells and tissues using imaging mass spectrometry. Image 1 • A critical review of recent advances in MS analysis of glycosphingolipidome. • Nomenclature and biological significance of glycosphingolipids are covered. • Comprehensive coverage of novel enrichment strategies for glycosphingolipids. • Extensive coverage of fragmentation techniques in glycosphingolipid analysis. • Labeling approaches for quantitation and perspectives on future directions. [ABSTRACT FROM AUTHOR] |
| Copyright of Analytica Chimica Acta is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 146056987 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Recent advances in the mass spectrometric analysis of glycosphingolipidome – A review. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Barrientos%2C+Rodell+C%2E%22">Barrientos, Rodell C.</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhang%2C+Qibin%22">Zhang, Qibin</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> q_zhang2@uncg.edu</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Analytica+Chimica+Acta%22">Analytica Chimica Acta</searchLink>. Oct2020, Vol. 1132, p134-155. 22p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Tandem+mass+spectrometry%22">Tandem mass spectrometry</searchLink><br /><searchLink fieldCode="DE" term="%22Ion+mobility+spectroscopy%22">Ion mobility spectroscopy</searchLink><br /><searchLink fieldCode="DE" term="%22Mass+spectrometry%22">Mass spectrometry</searchLink><br /><searchLink fieldCode="DE" term="%22Biological+nomenclature%22">Biological nomenclature</searchLink><br /><searchLink fieldCode="DE" term="%22Glycosphingolipids%22">Glycosphingolipids</searchLink><br /><searchLink fieldCode="DE" term="%22Isomers%22">Isomers</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Aberrant expression of glycosphingolipids has been implicated in a myriad of diseases, but our understanding of the strucural diversity, spatial distribution, and biological function of this class of biomolecules remains limited. These challenges partly stem from a lack of sensitive tools that can detect, identify, and quantify glycosphingolipids at the molecular level. Mass spectrometry has emerged as a powerful tool poised to address most of these challenges. Here, we review the recent developments in analytical glycosphingolipidomics with an emphasis on sample preparation, mass spectrometry and tandem mass spectrometry-based structural characterization, label-free and labeling-based quantification. We also discuss the nomenclature of glycosphingolipids, and emerging technologies like ion mobility spectrometry in differentiation of glycosphingolipid isomers. The intrinsic advantages and shortcomings of each method are carefully critiqued in line with an individual's research goals. Finally, future perspectives on analytical sphingolipidomics are stated, including a need for novel and more sensive methods in isomer separation, low abundance species detection, and profiling the spatial distribution of glycosphingolipid molecular species in cells and tissues using imaging mass spectrometry. Image 1 • A critical review of recent advances in MS analysis of glycosphingolipidome. • Nomenclature and biological significance of glycosphingolipids are covered. • Comprehensive coverage of novel enrichment strategies for glycosphingolipids. • Extensive coverage of fragmentation techniques in glycosphingolipid analysis. • Labeling approaches for quantitation and perspectives on future directions. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Analytica Chimica Acta is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.aca.2020.05.051 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 22 StartPage: 134 Subjects: – SubjectFull: Tandem mass spectrometry Type: general – SubjectFull: Ion mobility spectroscopy Type: general – SubjectFull: Mass spectrometry Type: general – SubjectFull: Biological nomenclature Type: general – SubjectFull: Glycosphingolipids Type: general – SubjectFull: Isomers Type: general Titles: – TitleFull: Recent advances in the mass spectrometric analysis of glycosphingolipidome – A review. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Barrientos, Rodell C. – PersonEntity: Name: NameFull: Zhang, Qibin IsPartOfRelationships: – BibEntity: Dates: – D: 02 M: 10 Text: Oct2020 Type: published Y: 2020 Identifiers: – Type: issn-print Value: 00032670 Numbering: – Type: volume Value: 1132 Titles: – TitleFull: Analytica Chimica Acta Type: main |
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