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Synergy effects of Methylomonas koyamae and Hyphomicrobium methylovorum under methanethiol stress.

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Title: Synergy effects of Methylomonas koyamae and Hyphomicrobium methylovorum under methanethiol stress.
Authors: Zhang, Xin1 (AUTHOR), Li, Hua-Jun2 (AUTHOR), Jiang, Lei1 (AUTHOR), Wang, Jing2 (AUTHOR), He, Ruo1,2 (AUTHOR) heruo@zju.edu.cn
Source: Applied Microbiology & Biotechnology. May2023, Vol. 107 Issue 9, p3099-3111. 13p.
Subjects: Methanethiol, Sulfur cycle, Volatile organic compounds, Biogeochemical cycles, Microbial communities, Methanotrophs, Ecosystems
Abstract: Methanotrophs are able to metabolize volatile organic sulfur compounds (VOSCs), excrete organic carbon during CH4 oxidation, and influence microbial community structure and function of the ecosystem. In return, microbial community structure and environmental factors can affect the growth metabolism of methanotrophs. In this study, Methylomonas koyamae and Hyphomicrobium methylovorum were used for model organisms, and methanethiol (MT) was chosen for a typical VOSC to investigate the synergy effects under VOSC stress. The results showed that when Hyphomicrobium methylovorum was co-cultured with Methylomonas koyamae in the medium with CH4 used as the carbon source, the co-culture had better MT tolerance relative to Methylomonas koyamae and oxidized all CH4 within 120 h, even at the initial MT concentration of 2000 mg m−3. The optimal co-culture ratios of Methylomonas koyamae to Hyphomicrobium methylovorum were 4:1–12:1. Although MT could be converted spontaneously to dimethyl disulfide (DMDS), H2S, and CS2 in air, faster losses of MT, DMDS, H2S, and CS2 were observed in each strain mono-culture and the co-culture. Compared with Hyphomicrobium methylovorum, MT was degraded more quickly in the Methylomonas koyamae culture. During the co-culture, the CH4 oxidation process of Methylomonas koyamae could provide carbon and energy sources for the growth of Hyphomicrobium methylovorum, while Hyphomicrobium methylovorum oxidized MT to help Methylomonas koyamae detoxify. These findings are helpful to understand the synergy effects of Methylomonas koyamae and Hyphomicrobium methylovorum under MT stress and enrich the role of methanotrophs in the sulfur biogeochemical cycle. Key points: • The co-culture of Methylomonas and Hyphomicrobium has better tolerance to CH3SH. • Methylomonas can provide carbon sources for the growth of Hyphomicrobium. • The co-culture of Methylomonas and Hyphomicrobium enhances the removal of CH4 and CH3SH. [ABSTRACT FROM AUTHOR]
Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Label: Title
  Group: Ti
  Data: Synergy effects of Methylomonas koyamae and Hyphomicrobium methylovorum under methanethiol stress.
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  Data: <searchLink fieldCode="AR" term="%22Zhang%2C+Xin%22">Zhang, Xin</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Li%2C+Hua-Jun%22">Li, Hua-Jun</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Jiang%2C+Lei%22">Jiang, Lei</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Jing%22">Wang, Jing</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22He%2C+Ruo%22">He, Ruo</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> heruo@zju.edu.cn</i>
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  Data: <searchLink fieldCode="JN" term="%22Applied+Microbiology+%26+Biotechnology%22">Applied Microbiology & Biotechnology</searchLink>. May2023, Vol. 107 Issue 9, p3099-3111. 13p.
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  Data: <searchLink fieldCode="DE" term="%22Methanethiol%22">Methanethiol</searchLink><br /><searchLink fieldCode="DE" term="%22Sulfur+cycle%22">Sulfur cycle</searchLink><br /><searchLink fieldCode="DE" term="%22Volatile+organic+compounds%22">Volatile organic compounds</searchLink><br /><searchLink fieldCode="DE" term="%22Biogeochemical+cycles%22">Biogeochemical cycles</searchLink><br /><searchLink fieldCode="DE" term="%22Microbial+communities%22">Microbial communities</searchLink><br /><searchLink fieldCode="DE" term="%22Methanotrophs%22">Methanotrophs</searchLink><br /><searchLink fieldCode="DE" term="%22Ecosystems%22">Ecosystems</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Methanotrophs are able to metabolize volatile organic sulfur compounds (VOSCs), excrete organic carbon during CH4 oxidation, and influence microbial community structure and function of the ecosystem. In return, microbial community structure and environmental factors can affect the growth metabolism of methanotrophs. In this study, Methylomonas koyamae and Hyphomicrobium methylovorum were used for model organisms, and methanethiol (MT) was chosen for a typical VOSC to investigate the synergy effects under VOSC stress. The results showed that when Hyphomicrobium methylovorum was co-cultured with Methylomonas koyamae in the medium with CH4 used as the carbon source, the co-culture had better MT tolerance relative to Methylomonas koyamae and oxidized all CH4 within 120 h, even at the initial MT concentration of 2000 mg m−3. The optimal co-culture ratios of Methylomonas koyamae to Hyphomicrobium methylovorum were 4:1–12:1. Although MT could be converted spontaneously to dimethyl disulfide (DMDS), H2S, and CS2 in air, faster losses of MT, DMDS, H2S, and CS2 were observed in each strain mono-culture and the co-culture. Compared with Hyphomicrobium methylovorum, MT was degraded more quickly in the Methylomonas koyamae culture. During the co-culture, the CH4 oxidation process of Methylomonas koyamae could provide carbon and energy sources for the growth of Hyphomicrobium methylovorum, while Hyphomicrobium methylovorum oxidized MT to help Methylomonas koyamae detoxify. These findings are helpful to understand the synergy effects of Methylomonas koyamae and Hyphomicrobium methylovorum under MT stress and enrich the role of methanotrophs in the sulfur biogeochemical cycle. Key points: • The co-culture of Methylomonas and Hyphomicrobium has better tolerance to CH3SH. • Methylomonas can provide carbon sources for the growth of Hyphomicrobium. • The co-culture of Methylomonas and Hyphomicrobium enhances the removal of CH4 and CH3SH. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1007/s00253-023-12472-w
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      – Code: eng
        Text: English
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        PageCount: 13
        StartPage: 3099
    Subjects:
      – SubjectFull: Methanethiol
        Type: general
      – SubjectFull: Sulfur cycle
        Type: general
      – SubjectFull: Volatile organic compounds
        Type: general
      – SubjectFull: Biogeochemical cycles
        Type: general
      – SubjectFull: Microbial communities
        Type: general
      – SubjectFull: Methanotrophs
        Type: general
      – SubjectFull: Ecosystems
        Type: general
    Titles:
      – TitleFull: Synergy effects of Methylomonas koyamae and Hyphomicrobium methylovorum under methanethiol stress.
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            NameFull: Zhang, Xin
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            NameFull: Li, Hua-Jun
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            NameFull: Jiang, Lei
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            NameFull: Wang, Jing
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            NameFull: He, Ruo
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            – D: 01
              M: 05
              Text: May2023
              Type: published
              Y: 2023
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              Value: 107
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            – TitleFull: Applied Microbiology & Biotechnology
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