View in EDS

Electrophoretic deposition of gentamicin and chitosan into titanium nanotubes to target periprosthetic joint infection.

Saved in:
Bibliographic Details
Title: Electrophoretic deposition of gentamicin and chitosan into titanium nanotubes to target periprosthetic joint infection.
Authors: Della Fara, Greta1 (AUTHOR), Markovics, Adrienn1 (AUTHOR) adrienn_markovics@rush.edu, Radice, Simona1 (AUTHOR), Hamilton, John L.1 (AUTHOR), Chiesa, Roberto2 (AUTHOR), Sturm, Andreas3 (AUTHOR), Angenendt, Katja3 (AUTHOR), Fischer, Alfons1,3 (AUTHOR), Wimmer, Markus A.1 (AUTHOR)
Source: Journal of Biomedical Materials Research, Part B: Applied Biomaterials. Sep2023, Vol. 111 Issue 9, p1697-1704. 8p.
Subjects: Joint infections, Electrophoretic deposition, Gentamicin, Nanotubes, Chitosan
Abstract: Periprosthetic joint infection (PJI) occurs in 1%–2% of primary total hip and knee arthroplasties; the rate can reach 20% in individuals at risk. Due to the low local bioavailability of systemic antibiotics and possible off‐target effects, localized drug delivery systems are of great importance. Our aim was the electrophoretic deposition (EPD) of gentamicin and chitosan in Titanium (Ti) nanotubes to establish a local, prolonged antibiotic delivery. Nanotubes were created on Ti wire with a two‐step anodization process. For drug deposition, EPD and the air‐dry methods were compared. For a prolonged drug release, gentamicin and crosslinked chitosan were deposited in a two‐step EPD process. Drug release was quantified by fractional volume sampling. The Ti wires were tested against Staphylococcus aureus by agar dilution and liquid culture methods. MC3T3‐E1 osteoblastic cell viability was determined with trypan blue. Nanotubes were characterized by a 100 nm diameter and 7 μm length. EPD allowed a higher amount of gentamicin deposited than the air‐dry method. Drug deposition was controllable by adjusting the voltage and duration of the EPD process. The crosslinked chitosan layer allowed diffusion‐driven release kinetics for up to 3 days. Gentamicin‐loaded Ti wires significantly inhibited bacterial growth and resulted in a larger inhibition zone compared to unloaded wires. Twenty‐four hours of incubation with loaded wires did not have a significant effect on osteoblast viability. Gentamicin‐loaded Ti nanotubes represent a promising approach for PJI prevention, as well as a valuable preclinical tool for the investigation of localized drug delivery systems created on Ti surface. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Biomedical Materials Research, Part B: Applied Biomaterials is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
  Availability: 0
Header DbId: egs
DbLabel: Engineering Source
An: 164683172
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Electrophoretic deposition of gentamicin and chitosan into titanium nanotubes to target periprosthetic joint infection.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Della+Fara%2C+Greta%22">Della Fara, Greta</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Markovics%2C+Adrienn%22">Markovics, Adrienn</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> adrienn_markovics@rush.edu</i><br /><searchLink fieldCode="AR" term="%22Radice%2C+Simona%22">Radice, Simona</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Hamilton%2C+John+L%2E%22">Hamilton, John L.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Chiesa%2C+Roberto%22">Chiesa, Roberto</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Sturm%2C+Andreas%22">Sturm, Andreas</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Angenendt%2C+Katja%22">Angenendt, Katja</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Fischer%2C+Alfons%22">Fischer, Alfons</searchLink><relatesTo>1,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wimmer%2C+Markus+A%2E%22">Wimmer, Markus A.</searchLink><relatesTo>1</relatesTo> (AUTHOR)
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Journal+of+Biomedical+Materials+Research%2C+Part+B%3A+Applied+Biomaterials%22">Journal of Biomedical Materials Research, Part B: Applied Biomaterials</searchLink>. Sep2023, Vol. 111 Issue 9, p1697-1704. 8p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Joint+infections%22">Joint infections</searchLink><br /><searchLink fieldCode="DE" term="%22Electrophoretic+deposition%22">Electrophoretic deposition</searchLink><br /><searchLink fieldCode="DE" term="%22Gentamicin%22">Gentamicin</searchLink><br /><searchLink fieldCode="DE" term="%22Nanotubes%22">Nanotubes</searchLink><br /><searchLink fieldCode="DE" term="%22Chitosan%22">Chitosan</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Periprosthetic joint infection (PJI) occurs in 1%–2% of primary total hip and knee arthroplasties; the rate can reach 20% in individuals at risk. Due to the low local bioavailability of systemic antibiotics and possible off‐target effects, localized drug delivery systems are of great importance. Our aim was the electrophoretic deposition (EPD) of gentamicin and chitosan in Titanium (Ti) nanotubes to establish a local, prolonged antibiotic delivery. Nanotubes were created on Ti wire with a two‐step anodization process. For drug deposition, EPD and the air‐dry methods were compared. For a prolonged drug release, gentamicin and crosslinked chitosan were deposited in a two‐step EPD process. Drug release was quantified by fractional volume sampling. The Ti wires were tested against Staphylococcus aureus by agar dilution and liquid culture methods. MC3T3‐E1 osteoblastic cell viability was determined with trypan blue. Nanotubes were characterized by a 100 nm diameter and 7 μm length. EPD allowed a higher amount of gentamicin deposited than the air‐dry method. Drug deposition was controllable by adjusting the voltage and duration of the EPD process. The crosslinked chitosan layer allowed diffusion‐driven release kinetics for up to 3 days. Gentamicin‐loaded Ti wires significantly inhibited bacterial growth and resulted in a larger inhibition zone compared to unloaded wires. Twenty‐four hours of incubation with loaded wires did not have a significant effect on osteoblast viability. Gentamicin‐loaded Ti nanotubes represent a promising approach for PJI prevention, as well as a valuable preclinical tool for the investigation of localized drug delivery systems created on Ti surface. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Biomedical Materials Research, Part B: Applied Biomaterials is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=164683172
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1002/jbm.b.35267
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 8
        StartPage: 1697
    Subjects:
      – SubjectFull: Joint infections
        Type: general
      – SubjectFull: Electrophoretic deposition
        Type: general
      – SubjectFull: Gentamicin
        Type: general
      – SubjectFull: Nanotubes
        Type: general
      – SubjectFull: Chitosan
        Type: general
    Titles:
      – TitleFull: Electrophoretic deposition of gentamicin and chitosan into titanium nanotubes to target periprosthetic joint infection.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Della Fara, Greta
      – PersonEntity:
          Name:
            NameFull: Markovics, Adrienn
      – PersonEntity:
          Name:
            NameFull: Radice, Simona
      – PersonEntity:
          Name:
            NameFull: Hamilton, John L.
      – PersonEntity:
          Name:
            NameFull: Chiesa, Roberto
      – PersonEntity:
          Name:
            NameFull: Sturm, Andreas
      – PersonEntity:
          Name:
            NameFull: Angenendt, Katja
      – PersonEntity:
          Name:
            NameFull: Fischer, Alfons
      – PersonEntity:
          Name:
            NameFull: Wimmer, Markus A.
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 09
              Text: Sep2023
              Type: published
              Y: 2023
          Identifiers:
            – Type: issn-print
              Value: 15524973
          Numbering:
            – Type: volume
              Value: 111
            – Type: issue
              Value: 9
          Titles:
            – TitleFull: Journal of Biomedical Materials Research, Part B: Applied Biomaterials
              Type: main
ResultId 1