View in EDS

Evaluation and characterization of an endosperm-specific sbeIIa promoter in wheat.

Saved in:
Bibliographic Details
Title: Evaluation and characterization of an endosperm-specific sbeIIa promoter in wheat.
Authors: Miao Hongmei1, Fleming, Joy E.2 jef@psmail.net, Lü Debing1, Han Jinfeng1
Source: Chinese Science Bulletin. Mar2004, Vol. 49 Issue 6, p579-585. 7p. 4 Color Photographs, 2 Diagrams, 1 Chart, 1 Graph.
Subjects: Wheat, Starch, Grain, Biochemical engineering, Biochemistry
Abstract: Starch, the main component of the wheat grain, is the product of a complex biochemical pathway. The sbeIIa gene plays a key role in controlling the synthesis of starch, in particular, the biosynthesis of amylopectin, in maturing wheat grain. To investigate its regulatory mechanisms and endosperm-specific expression pattern, the sbeIIa promoter (3094 bp in length) was cloned using APCR and sequenced. The effect of a series of deletions was studied using a GUS transient assay system. Results showed that the 3094 bp sequence (sbe.g construct) exhibited full stable promoting activity and that the activities of 5' or 3' deletions reduced levels of GUS expression. Some constructs with internal deletions showed only weak activity, however, sbe.e, with a deletion from -1579—-1210 bp resulted in higher levels of expression than the full-length promoter sequence, sbe.g. This indicates that motifs such as the -300 bp element, G-box and/or P-box act as positive elements and are necessary in determining the promoter's endosperm-specific pattern and that negative repressor elements or motifs may also be present within the -1579—-1210 bp sequence. The age of wheat endosperm tissue used in the GUS-transient assay system is shown to be of significant importance. [ABSTRACT FROM AUTHOR]
Copyright of Chinese Science Bulletin is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
Description
Abstract:Starch, the main component of the wheat grain, is the product of a complex biochemical pathway. The sbeIIa gene plays a key role in controlling the synthesis of starch, in particular, the biosynthesis of amylopectin, in maturing wheat grain. To investigate its regulatory mechanisms and endosperm-specific expression pattern, the sbeIIa promoter (3094 bp in length) was cloned using APCR and sequenced. The effect of a series of deletions was studied using a GUS transient assay system. Results showed that the 3094 bp sequence (sbe.g construct) exhibited full stable promoting activity and that the activities of 5' or 3' deletions reduced levels of GUS expression. Some constructs with internal deletions showed only weak activity, however, sbe.e, with a deletion from -1579—-1210 bp resulted in higher levels of expression than the full-length promoter sequence, sbe.g. This indicates that motifs such as the -300 bp element, G-box and/or P-box act as positive elements and are necessary in determining the promoter's endosperm-specific pattern and that negative repressor elements or motifs may also be present within the -1579—-1210 bp sequence. The age of wheat endosperm tissue used in the GUS-transient assay system is shown to be of significant importance. [ABSTRACT FROM AUTHOR]
ISSN:10016538
DOI:10.1360/03wc0505