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Plasma-activated medium suppresses proliferation and migration of human lung cancer cells by regulating PI3K/AKT-Wnt signaling pathway.

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Title: Plasma-activated medium suppresses proliferation and migration of human lung cancer cells by regulating PI3K/AKT-Wnt signaling pathway.
Authors: Sun, Zhidan1,2 (AUTHOR), Ding, Chenglong1,2 (AUTHOR), Wang, Yuhan1,2 (AUTHOR), Zhou, Han2 (AUTHOR), Song, Wencheng1,2,3,4 (AUTHOR) wencsong@cmpt.ac.cn
Source: Journal of Bioscience & Bioengineering. Jan2025, Vol. 139 Issue 1, p60-69. 10p.
Subjects: Reactive oxygen species, Reactive nitrogen species, Cancer cell growth, Cancer-related mortality, Cell migration
Abstract: The main causes of high mortality in lung cancer patients are the malignant growth and migration of cancer cells. This study aims to investigate the underlying mechanisms of low-temperature plasma-activated medium (PAM) treating human lung cancer (HLC). Changes in the levels of reactive oxygen and nitrogen species both inside and outside the cells were evaluated. Our results showed that prolonged PAM exposure decreased cell viability, raised intracellular reactive oxygen species levels, and hindered cell migration while reducing mitochondrial membrane potential. Protein analysis revealed PAM increased GSK-3β and p-β-catenin expression but decreased PI3K, AKT, p-AKT, p-GSK-3β, Wnt, and β-catenin levels, thereby inhibiting the epithelial–mesenchymal transition. These findings suggest PAM suppresses HLC cells proliferation and migration by blocking the PI3K/AKT-Wnt pathway. The study will provide a valuable theoretical basis for future low-temperature plasma treatment, thereby improving the survival rates and prognosis of lung cancer. [Display omitted] • Role of bioactive components generated by low-temperature plasma-activated medium (PAM). • Investigation into the suppressive influence of PAM on the proliferation and motility of human lung cancer (HLC) cells. • Utilization of microfluidic chip technology to assess the biological impact of PAM on HLC cells. • Exploration of the new intrinsic molecular mechanism of PAM on HLC cells. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
Description
Abstract:The main causes of high mortality in lung cancer patients are the malignant growth and migration of cancer cells. This study aims to investigate the underlying mechanisms of low-temperature plasma-activated medium (PAM) treating human lung cancer (HLC). Changes in the levels of reactive oxygen and nitrogen species both inside and outside the cells were evaluated. Our results showed that prolonged PAM exposure decreased cell viability, raised intracellular reactive oxygen species levels, and hindered cell migration while reducing mitochondrial membrane potential. Protein analysis revealed PAM increased GSK-3β and p-β-catenin expression but decreased PI3K, AKT, p-AKT, p-GSK-3β, Wnt, and β-catenin levels, thereby inhibiting the epithelial–mesenchymal transition. These findings suggest PAM suppresses HLC cells proliferation and migration by blocking the PI3K/AKT-Wnt pathway. The study will provide a valuable theoretical basis for future low-temperature plasma treatment, thereby improving the survival rates and prognosis of lung cancer. [Display omitted] • Role of bioactive components generated by low-temperature plasma-activated medium (PAM). • Investigation into the suppressive influence of PAM on the proliferation and motility of human lung cancer (HLC) cells. • Utilization of microfluidic chip technology to assess the biological impact of PAM on HLC cells. • Exploration of the new intrinsic molecular mechanism of PAM on HLC cells. [ABSTRACT FROM AUTHOR]
ISSN:13891723
DOI:10.1016/j.jbiosc.2024.10.002