View in EDS

Plasma-activated medium suppresses proliferation and migration of human lung cancer cells by regulating PI3K/AKT-Wnt signaling pathway.

Saved in:
Bibliographic Details
Title: Plasma-activated medium suppresses proliferation and migration of human lung cancer cells by regulating PI3K/AKT-Wnt signaling pathway.
Authors: Sun, Zhidan1,2 (AUTHOR), Ding, Chenglong1,2 (AUTHOR), Wang, Yuhan1,2 (AUTHOR), Zhou, Han2 (AUTHOR), Song, Wencheng1,2,3,4 (AUTHOR) wencsong@cmpt.ac.cn
Source: Journal of Bioscience & Bioengineering. Jan2025, Vol. 139 Issue 1, p60-69. 10p.
Subjects: Reactive oxygen species, Reactive nitrogen species, Cancer cell growth, Cancer-related mortality, Cell migration
Abstract: The main causes of high mortality in lung cancer patients are the malignant growth and migration of cancer cells. This study aims to investigate the underlying mechanisms of low-temperature plasma-activated medium (PAM) treating human lung cancer (HLC). Changes in the levels of reactive oxygen and nitrogen species both inside and outside the cells were evaluated. Our results showed that prolonged PAM exposure decreased cell viability, raised intracellular reactive oxygen species levels, and hindered cell migration while reducing mitochondrial membrane potential. Protein analysis revealed PAM increased GSK-3β and p-β-catenin expression but decreased PI3K, AKT, p-AKT, p-GSK-3β, Wnt, and β-catenin levels, thereby inhibiting the epithelial–mesenchymal transition. These findings suggest PAM suppresses HLC cells proliferation and migration by blocking the PI3K/AKT-Wnt pathway. The study will provide a valuable theoretical basis for future low-temperature plasma treatment, thereby improving the survival rates and prognosis of lung cancer. [Display omitted] • Role of bioactive components generated by low-temperature plasma-activated medium (PAM). • Investigation into the suppressive influence of PAM on the proliferation and motility of human lung cancer (HLC) cells. • Utilization of microfluidic chip technology to assess the biological impact of PAM on HLC cells. • Exploration of the new intrinsic molecular mechanism of PAM on HLC cells. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Bioscience & Bioengineering is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
  Availability: 0
Header DbId: egs
DbLabel: Engineering Source
An: 181682259
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Plasma-activated medium suppresses proliferation and migration of human lung cancer cells by regulating PI3K/AKT-Wnt signaling pathway.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Sun%2C+Zhidan%22">Sun, Zhidan</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ding%2C+Chenglong%22">Ding, Chenglong</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Yuhan%22">Wang, Yuhan</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhou%2C+Han%22">Zhou, Han</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Song%2C+Wencheng%22">Song, Wencheng</searchLink><relatesTo>1,2,3,4</relatesTo> (AUTHOR)<i> wencsong@cmpt.ac.cn</i>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Journal+of+Bioscience+%26+Bioengineering%22">Journal of Bioscience & Bioengineering</searchLink>. Jan2025, Vol. 139 Issue 1, p60-69. 10p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Reactive+oxygen+species%22">Reactive oxygen species</searchLink><br /><searchLink fieldCode="DE" term="%22Reactive+nitrogen+species%22">Reactive nitrogen species</searchLink><br /><searchLink fieldCode="DE" term="%22Cancer+cell+growth%22">Cancer cell growth</searchLink><br /><searchLink fieldCode="DE" term="%22Cancer-related+mortality%22">Cancer-related mortality</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+migration%22">Cell migration</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: The main causes of high mortality in lung cancer patients are the malignant growth and migration of cancer cells. This study aims to investigate the underlying mechanisms of low-temperature plasma-activated medium (PAM) treating human lung cancer (HLC). Changes in the levels of reactive oxygen and nitrogen species both inside and outside the cells were evaluated. Our results showed that prolonged PAM exposure decreased cell viability, raised intracellular reactive oxygen species levels, and hindered cell migration while reducing mitochondrial membrane potential. Protein analysis revealed PAM increased GSK-3β and p-β-catenin expression but decreased PI3K, AKT, p-AKT, p-GSK-3β, Wnt, and β-catenin levels, thereby inhibiting the epithelial–mesenchymal transition. These findings suggest PAM suppresses HLC cells proliferation and migration by blocking the PI3K/AKT-Wnt pathway. The study will provide a valuable theoretical basis for future low-temperature plasma treatment, thereby improving the survival rates and prognosis of lung cancer. [Display omitted] • Role of bioactive components generated by low-temperature plasma-activated medium (PAM). • Investigation into the suppressive influence of PAM on the proliferation and motility of human lung cancer (HLC) cells. • Utilization of microfluidic chip technology to assess the biological impact of PAM on HLC cells. • Exploration of the new intrinsic molecular mechanism of PAM on HLC cells. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Bioscience & Bioengineering is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=181682259
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1016/j.jbiosc.2024.10.002
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 10
        StartPage: 60
    Subjects:
      – SubjectFull: Reactive oxygen species
        Type: general
      – SubjectFull: Reactive nitrogen species
        Type: general
      – SubjectFull: Cancer cell growth
        Type: general
      – SubjectFull: Cancer-related mortality
        Type: general
      – SubjectFull: Cell migration
        Type: general
    Titles:
      – TitleFull: Plasma-activated medium suppresses proliferation and migration of human lung cancer cells by regulating PI3K/AKT-Wnt signaling pathway.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Sun, Zhidan
      – PersonEntity:
          Name:
            NameFull: Ding, Chenglong
      – PersonEntity:
          Name:
            NameFull: Wang, Yuhan
      – PersonEntity:
          Name:
            NameFull: Zhou, Han
      – PersonEntity:
          Name:
            NameFull: Song, Wencheng
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 01
              Text: Jan2025
              Type: published
              Y: 2025
          Identifiers:
            – Type: issn-print
              Value: 13891723
          Numbering:
            – Type: volume
              Value: 139
            – Type: issue
              Value: 1
          Titles:
            – TitleFull: Journal of Bioscience & Bioengineering
              Type: main
ResultId 1