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Enhanced production of ergothioneine in Aspergillus oryzae.

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Title: Enhanced production of ergothioneine in Aspergillus oryzae.
Authors: Wang, Lihong1,2 (AUTHOR), Tian, Xueqin1,2 (AUTHOR), Xue, Pinghong1,2 (AUTHOR), Deng, Yunhong1,2 (AUTHOR), Gao, Rui1,2 (AUTHOR) gaoruineau@163.com, Hu, Zhihong1,2 (AUTHOR) huzhihong426@163.com
Source: Applied Microbiology & Biotechnology. 6/10/2025, Vol. 109 Issue 1, p1-11. 11p.
Subjects: Koji, Neurospora crassa, Edible fungi, Filamentous fungi, Peroxisomes
Abstract: Ergothioneine (EGT) is a rare amino acid with potent antioxidant and anti-inflammatory properties, with a wide range of applications in food, cosmetics, and medicine. In the present study, Aspergillus oryzae, a common edible fungus, was engineered as an optimal host for EGT production. Moreover, two endogenous genes involved in EGT biosynthesis were characterized. The homolog AoEgt1 was shown to be localized in the vacuoles, whereas the homolog AoEgt2 was found in the peroxisomes. Overexpression of EGT biosynthetic genes from different organisms enhanced EGT production, yielding 15.17 mg EGT/g of dry weight. Using glucose as the carbon source and supplementing methionine (Met) as a precursor further increased EGT production to 20.03 mg EGT/g of dry weight, constituting an eight-fold increase compared to the wild-type strain. This study discusses the successful construction of a high-yielding A. oryzae strain for EGT biosynthesis, providing a novel strategy for efficient EGT synthesis. Key points: • Two newly described homologs, AoEgt1 and AoEgt2, were identified in A. oryzae. • AoEgt1 and AoEgt2 were found to contribute to EGT biosynthesis. • EGT production was significantly increased by overexpression of Egt1 and Egt2. • Glucose and Met supplementation in the medium increased EGT production. [ABSTRACT FROM AUTHOR]
Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Enhanced production of ergothioneine in Aspergillus oryzae.
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  Data: <searchLink fieldCode="AR" term="%22Wang%2C+Lihong%22">Wang, Lihong</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Tian%2C+Xueqin%22">Tian, Xueqin</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Xue%2C+Pinghong%22">Xue, Pinghong</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Deng%2C+Yunhong%22">Deng, Yunhong</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Gao%2C+Rui%22">Gao, Rui</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> gaoruineau@163.com</i><br /><searchLink fieldCode="AR" term="%22Hu%2C+Zhihong%22">Hu, Zhihong</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> huzhihong426@163.com</i>
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  Data: <searchLink fieldCode="JN" term="%22Applied+Microbiology+%26+Biotechnology%22">Applied Microbiology & Biotechnology</searchLink>. 6/10/2025, Vol. 109 Issue 1, p1-11. 11p.
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  Data: <searchLink fieldCode="DE" term="%22Koji%22">Koji</searchLink><br /><searchLink fieldCode="DE" term="%22Neurospora+crassa%22">Neurospora crassa</searchLink><br /><searchLink fieldCode="DE" term="%22Edible+fungi%22">Edible fungi</searchLink><br /><searchLink fieldCode="DE" term="%22Filamentous+fungi%22">Filamentous fungi</searchLink><br /><searchLink fieldCode="DE" term="%22Peroxisomes%22">Peroxisomes</searchLink>
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  Data: Ergothioneine (EGT) is a rare amino acid with potent antioxidant and anti-inflammatory properties, with a wide range of applications in food, cosmetics, and medicine. In the present study, Aspergillus oryzae, a common edible fungus, was engineered as an optimal host for EGT production. Moreover, two endogenous genes involved in EGT biosynthesis were characterized. The homolog AoEgt1 was shown to be localized in the vacuoles, whereas the homolog AoEgt2 was found in the peroxisomes. Overexpression of EGT biosynthetic genes from different organisms enhanced EGT production, yielding 15.17 mg EGT/g of dry weight. Using glucose as the carbon source and supplementing methionine (Met) as a precursor further increased EGT production to 20.03 mg EGT/g of dry weight, constituting an eight-fold increase compared to the wild-type strain. This study discusses the successful construction of a high-yielding A. oryzae strain for EGT biosynthesis, providing a novel strategy for efficient EGT synthesis. Key points: • Two newly described homologs, AoEgt1 and AoEgt2, were identified in A. oryzae. • AoEgt1 and AoEgt2 were found to contribute to EGT biosynthesis. • EGT production was significantly increased by overexpression of Egt1 and Egt2. • Glucose and Met supplementation in the medium increased EGT production. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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              Text: 6/10/2025
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