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A Y-shape-structured photoelectrochemical biosensor based on Bi4NbO8Cl/TiO2 heterojunction and enzyme-mediated electrocatalysis for sensitive and selective determination of microRNA in serum.

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Title: A Y-shape-structured photoelectrochemical biosensor based on Bi4NbO8Cl/TiO2 heterojunction and enzyme-mediated electrocatalysis for sensitive and selective determination of microRNA in serum.
Authors: Zhong, Yu1 (AUTHOR), Xu, Zhi-Wei1 (AUTHOR), Cheng, Zhang-Jian1 (AUTHOR), Liu, Ai-Lin1 (AUTHOR) ailinliu@fjmu.edu.cn, Lei, Yun1 (AUTHOR) leiyun@fjmu.edu.cn
Source: Microchemical Journal. Jul2025, Vol. 214, pN.PAG-N.PAG. 1p.
Subjects: Electron donors, Electron transport, Titanium dioxide, Detection limit, Biosensors, Alkaline phosphatase
Abstract: [Display omitted] • A Y-shape-structured PEC biosensor is constructed using dual signal amplification for miRNA-222 assay in serum. • A Y-shaped junction structure demonstrates salient selectivity and anti-interference capacity. • Bi 4 NbO 8 Cl/TiO 2 heterojunction enhances PEC biosensor performance. • The proposed PEC biosensor displays potential applications in tumor-related miRNAs assay of the clinical diagnosis. Accurate and sensitive detection of microRNAs (miRNAs) in tumorigenesis has posed a great challenge, as its characteristics of ultra-low abundance and high sequence homology. In this approach, a highly sensitive and selective Y-shape-structured photoelectrochemical (PEC) biosensor coupled with dual signal amplification strategy was constructed with introduction of heterojunction nanocomposite and in situ enzymatic generation of electron donor. Specifically, Bi 4 NbO 8 Cl/TiO 2 heterojunction allowed for efficient electron transport, further achieved the electrons-holes pairs separation, and thus greatly improved photocurrent intensity. Alkaline phosphatase (ALP) modified with auxiliary probes catalyzes hydrolysis of ascorbic acid 2-phosphate (AAP) to in situ generate ascorbic acid (AA) as electron donors for further signal amplification. Furthermore, hairpin capture probes with rigid structure were immobilized upright on electrode surface and stable Y-shaped junction configurations were formed away from the electrode surface, which was beneficial to avoid probes' self-entanglement, improve the hybridization efficiency and enhance the recognition ability. As a proof-of-concept, the Y-shape-structured PEC biosensor coupled with dual signal amplification tactic achieves accurate quantification of miRNA-222 (papillary thyroid carcinoma-relevant biomarker) with salient selectivity, rapid response and low detection limit (0.15 pM, S/N = 3). The proposed PEC biosensor displays potential applications in tumor-related miRNAs assay for tumor prediction, diagnosis and prognosis. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
Description
Abstract:[Display omitted] • A Y-shape-structured PEC biosensor is constructed using dual signal amplification for miRNA-222 assay in serum. • A Y-shaped junction structure demonstrates salient selectivity and anti-interference capacity. • Bi 4 NbO 8 Cl/TiO 2 heterojunction enhances PEC biosensor performance. • The proposed PEC biosensor displays potential applications in tumor-related miRNAs assay of the clinical diagnosis. Accurate and sensitive detection of microRNAs (miRNAs) in tumorigenesis has posed a great challenge, as its characteristics of ultra-low abundance and high sequence homology. In this approach, a highly sensitive and selective Y-shape-structured photoelectrochemical (PEC) biosensor coupled with dual signal amplification strategy was constructed with introduction of heterojunction nanocomposite and in situ enzymatic generation of electron donor. Specifically, Bi 4 NbO 8 Cl/TiO 2 heterojunction allowed for efficient electron transport, further achieved the electrons-holes pairs separation, and thus greatly improved photocurrent intensity. Alkaline phosphatase (ALP) modified with auxiliary probes catalyzes hydrolysis of ascorbic acid 2-phosphate (AAP) to in situ generate ascorbic acid (AA) as electron donors for further signal amplification. Furthermore, hairpin capture probes with rigid structure were immobilized upright on electrode surface and stable Y-shaped junction configurations were formed away from the electrode surface, which was beneficial to avoid probes' self-entanglement, improve the hybridization efficiency and enhance the recognition ability. As a proof-of-concept, the Y-shape-structured PEC biosensor coupled with dual signal amplification tactic achieves accurate quantification of miRNA-222 (papillary thyroid carcinoma-relevant biomarker) with salient selectivity, rapid response and low detection limit (0.15 pM, S/N = 3). The proposed PEC biosensor displays potential applications in tumor-related miRNAs assay for tumor prediction, diagnosis and prognosis. [ABSTRACT FROM AUTHOR]
ISSN:0026265X
DOI:10.1016/j.microc.2025.113954