A Y-shape-structured photoelectrochemical biosensor based on Bi4NbO8Cl/TiO2 heterojunction and enzyme-mediated electrocatalysis for sensitive and selective determination of microRNA in serum.
Saved in:
| Title: | A Y-shape-structured photoelectrochemical biosensor based on Bi4NbO8Cl/TiO2 heterojunction and enzyme-mediated electrocatalysis for sensitive and selective determination of microRNA in serum. |
|---|---|
| Authors: | Zhong, Yu1 (AUTHOR), Xu, Zhi-Wei1 (AUTHOR), Cheng, Zhang-Jian1 (AUTHOR), Liu, Ai-Lin1 (AUTHOR) ailinliu@fjmu.edu.cn, Lei, Yun1 (AUTHOR) leiyun@fjmu.edu.cn |
| Source: | Microchemical Journal. Jul2025, Vol. 214, pN.PAG-N.PAG. 1p. |
| Subjects: | Electron donors, Electron transport, Titanium dioxide, Detection limit, Biosensors, Alkaline phosphatase |
| Abstract: | [Display omitted] • A Y-shape-structured PEC biosensor is constructed using dual signal amplification for miRNA-222 assay in serum. • A Y-shaped junction structure demonstrates salient selectivity and anti-interference capacity. • Bi 4 NbO 8 Cl/TiO 2 heterojunction enhances PEC biosensor performance. • The proposed PEC biosensor displays potential applications in tumor-related miRNAs assay of the clinical diagnosis. Accurate and sensitive detection of microRNAs (miRNAs) in tumorigenesis has posed a great challenge, as its characteristics of ultra-low abundance and high sequence homology. In this approach, a highly sensitive and selective Y-shape-structured photoelectrochemical (PEC) biosensor coupled with dual signal amplification strategy was constructed with introduction of heterojunction nanocomposite and in situ enzymatic generation of electron donor. Specifically, Bi 4 NbO 8 Cl/TiO 2 heterojunction allowed for efficient electron transport, further achieved the electrons-holes pairs separation, and thus greatly improved photocurrent intensity. Alkaline phosphatase (ALP) modified with auxiliary probes catalyzes hydrolysis of ascorbic acid 2-phosphate (AAP) to in situ generate ascorbic acid (AA) as electron donors for further signal amplification. Furthermore, hairpin capture probes with rigid structure were immobilized upright on electrode surface and stable Y-shaped junction configurations were formed away from the electrode surface, which was beneficial to avoid probes' self-entanglement, improve the hybridization efficiency and enhance the recognition ability. As a proof-of-concept, the Y-shape-structured PEC biosensor coupled with dual signal amplification tactic achieves accurate quantification of miRNA-222 (papillary thyroid carcinoma-relevant biomarker) with salient selectivity, rapid response and low detection limit (0.15 pM, S/N = 3). The proposed PEC biosensor displays potential applications in tumor-related miRNAs assay for tumor prediction, diagnosis and prognosis. [ABSTRACT FROM AUTHOR] |
| Copyright of Microchemical Journal is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
|---|---|
| Header | DbId: egs DbLabel: Engineering Source An: 185873789 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: A Y-shape-structured photoelectrochemical biosensor based on Bi4NbO8Cl/TiO2 heterojunction and enzyme-mediated electrocatalysis for sensitive and selective determination of microRNA in serum. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Zhong%2C+Yu%22">Zhong, Yu</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Xu%2C+Zhi-Wei%22">Xu, Zhi-Wei</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Cheng%2C+Zhang-Jian%22">Cheng, Zhang-Jian</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Liu%2C+Ai-Lin%22">Liu, Ai-Lin</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> ailinliu@fjmu.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Lei%2C+Yun%22">Lei, Yun</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> leiyun@fjmu.edu.cn</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Microchemical+Journal%22">Microchemical Journal</searchLink>. Jul2025, Vol. 214, pN.PAG-N.PAG. 1p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Electron+donors%22">Electron donors</searchLink><br /><searchLink fieldCode="DE" term="%22Electron+transport%22">Electron transport</searchLink><br /><searchLink fieldCode="DE" term="%22Titanium+dioxide%22">Titanium dioxide</searchLink><br /><searchLink fieldCode="DE" term="%22Detection+limit%22">Detection limit</searchLink><br /><searchLink fieldCode="DE" term="%22Biosensors%22">Biosensors</searchLink><br /><searchLink fieldCode="DE" term="%22Alkaline+phosphatase%22">Alkaline phosphatase</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: [Display omitted] • A Y-shape-structured PEC biosensor is constructed using dual signal amplification for miRNA-222 assay in serum. • A Y-shaped junction structure demonstrates salient selectivity and anti-interference capacity. • Bi 4 NbO 8 Cl/TiO 2 heterojunction enhances PEC biosensor performance. • The proposed PEC biosensor displays potential applications in tumor-related miRNAs assay of the clinical diagnosis. Accurate and sensitive detection of microRNAs (miRNAs) in tumorigenesis has posed a great challenge, as its characteristics of ultra-low abundance and high sequence homology. In this approach, a highly sensitive and selective Y-shape-structured photoelectrochemical (PEC) biosensor coupled with dual signal amplification strategy was constructed with introduction of heterojunction nanocomposite and in situ enzymatic generation of electron donor. Specifically, Bi 4 NbO 8 Cl/TiO 2 heterojunction allowed for efficient electron transport, further achieved the electrons-holes pairs separation, and thus greatly improved photocurrent intensity. Alkaline phosphatase (ALP) modified with auxiliary probes catalyzes hydrolysis of ascorbic acid 2-phosphate (AAP) to in situ generate ascorbic acid (AA) as electron donors for further signal amplification. Furthermore, hairpin capture probes with rigid structure were immobilized upright on electrode surface and stable Y-shaped junction configurations were formed away from the electrode surface, which was beneficial to avoid probes' self-entanglement, improve the hybridization efficiency and enhance the recognition ability. As a proof-of-concept, the Y-shape-structured PEC biosensor coupled with dual signal amplification tactic achieves accurate quantification of miRNA-222 (papillary thyroid carcinoma-relevant biomarker) with salient selectivity, rapid response and low detection limit (0.15 pM, S/N = 3). The proposed PEC biosensor displays potential applications in tumor-related miRNAs assay for tumor prediction, diagnosis and prognosis. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Microchemical Journal is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=185873789 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.microc.2025.113954 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 1 StartPage: N.PAG Subjects: – SubjectFull: Electron donors Type: general – SubjectFull: Electron transport Type: general – SubjectFull: Titanium dioxide Type: general – SubjectFull: Detection limit Type: general – SubjectFull: Biosensors Type: general – SubjectFull: Alkaline phosphatase Type: general Titles: – TitleFull: A Y-shape-structured photoelectrochemical biosensor based on Bi4NbO8Cl/TiO2 heterojunction and enzyme-mediated electrocatalysis for sensitive and selective determination of microRNA in serum. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Zhong, Yu – PersonEntity: Name: NameFull: Xu, Zhi-Wei – PersonEntity: Name: NameFull: Cheng, Zhang-Jian – PersonEntity: Name: NameFull: Liu, Ai-Lin – PersonEntity: Name: NameFull: Lei, Yun IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 07 Text: Jul2025 Type: published Y: 2025 Identifiers: – Type: issn-print Value: 0026265X Numbering: – Type: volume Value: 214 Titles: – TitleFull: Microchemical Journal Type: main |
| ResultId | 1 |
