Characterization and chemical modification of a Bacillus velezensis SN-1 exopolysaccharide, rEPS-2.
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| Title: | Characterization and chemical modification of a Bacillus velezensis SN-1 exopolysaccharide, rEPS-2. |
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| Authors: | Wu, Rina1 (AUTHOR) wrn6956@163.com, Liu, Yao2 (AUTHOR) liuyao2605@163.com, Gao, Yuzhe2 (AUTHOR), Yang, Xiaofei2 (AUTHOR), Yang, Qingyu2 (AUTHOR) yangqy0311@163.com, Cao, Chengxu2 (AUTHOR) caochengxu@synu.edu.cn |
| Source: | European Food Research & Technology. Aug2025, Vol. 251 Issue 8, p2161-2171. 11p. |
| Subjects: | Microbial polysaccharides, Cancer treatment, Oxidant status, Chemical engineering, Antioxidants, Structure-activity relationships |
| Abstract: | This study developed three structurally modified derivatives (acetylated rEPS-2 A, phosphorylated rEPS-2P, and carboxymethylated rEPS-2 C) from rEPS-2, a bioactive exopolysaccharide produced by Bacillus velezensis SN-1. Comprehensive characterization using gel permeation chromatography, FT-IR, and NMR spectroscopy confirmed successful functional group incorporation, while SEM and TGA analyses revealed significant alterations in surface morphology and thermal stability. Biological evaluation demonstrated that carboxymethylated rEPS-2 C exhibited markedly enhanced antioxidant capacity (showing 28.6% increase in DPPH scavenging activity compared to native rEPS-2) and superior antitumor effects (with 75.35% inhibition against HepG-2 cells), outperforming other derivatives. These findings highlight the structure-activity relationship of polysaccharide modifications and position rEPS-2 C as a promising dual-functional agent for oxidative stress-related disorders and cancer therapy applications. [ABSTRACT FROM AUTHOR] |
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| Database: | Engineering Source |
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| Abstract: | This study developed three structurally modified derivatives (acetylated rEPS-2 A, phosphorylated rEPS-2P, and carboxymethylated rEPS-2 C) from rEPS-2, a bioactive exopolysaccharide produced by Bacillus velezensis SN-1. Comprehensive characterization using gel permeation chromatography, FT-IR, and NMR spectroscopy confirmed successful functional group incorporation, while SEM and TGA analyses revealed significant alterations in surface morphology and thermal stability. Biological evaluation demonstrated that carboxymethylated rEPS-2 C exhibited markedly enhanced antioxidant capacity (showing 28.6% increase in DPPH scavenging activity compared to native rEPS-2) and superior antitumor effects (with 75.35% inhibition against HepG-2 cells), outperforming other derivatives. These findings highlight the structure-activity relationship of polysaccharide modifications and position rEPS-2 C as a promising dual-functional agent for oxidative stress-related disorders and cancer therapy applications. [ABSTRACT FROM AUTHOR] |
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| ISSN: | 14382377 |
| DOI: | 10.1007/s00217-025-04748-4 |
