One-Step Immunoassay of Alpha-Fetoprotein Constructed by Silicon-Quantum-Dot-Loaded Porous Gold Nanoshells.
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| Title: | One-Step Immunoassay of Alpha-Fetoprotein Constructed by Silicon-Quantum-Dot-Loaded Porous Gold Nanoshells. |
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| Authors: | Lu, Xiaoling1 (AUTHOR), Shen, Chao1,2 (AUTHOR), Long, You1 (AUTHOR), Zhang, Song1,2 (AUTHOR), Chen, Fang2 (AUTHOR), Chen, Nan2 (AUTHOR), Huang, Chenghong1 (AUTHOR) |
| Source: | Nanomaterials (2079-4991). Apr2026, Vol. 16 Issue 8, p479. 16p. |
| Subjects: | Immunoassay, Alpha fetoproteins, Hepatocellular carcinoma, Fluorescence, Semiconductor quantum dots, Nanotechnology, Gold nanoparticles, Tumor markers |
| Abstract: | Alpha-fetoprotein (AFP) is widely utilized for auxiliary diagnosis of primary hepatocellular carcinoma. Therefore, the development of a facile immunosensor is essential for clinical applications. This study aims to develop a simple immunoassay for AFP detection. By incorporating silicon quantum dots (SiQDs) into etching hollow gold nanoshells (EHGNs) via precise nanomanipulation, we designed molecular probes based on SiQDs@EHGNs complex immobilized capture antibodies, which can convert the antigen/antibody binding process into fluorescent divergence signals for AFP measurement. This strategy enabled one-step fluorescence sensing for AFP detection with a linear range of 3.125–200.0 ng/mL and LOD of 0.234 ng/mL. The detection results of 15 clinical serum real samples demonstrated a 93.7% correlation with the market-accepted ECLIA method. The proposed method take advantages of simplicity and rapid response, offering a novel approach for tumor marker analysis with significant potential. [ABSTRACT FROM AUTHOR] |
| Copyright of Nanomaterials (2079-4991) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Abstract: | Alpha-fetoprotein (AFP) is widely utilized for auxiliary diagnosis of primary hepatocellular carcinoma. Therefore, the development of a facile immunosensor is essential for clinical applications. This study aims to develop a simple immunoassay for AFP detection. By incorporating silicon quantum dots (SiQDs) into etching hollow gold nanoshells (EHGNs) via precise nanomanipulation, we designed molecular probes based on SiQDs@EHGNs complex immobilized capture antibodies, which can convert the antigen/antibody binding process into fluorescent divergence signals for AFP measurement. This strategy enabled one-step fluorescence sensing for AFP detection with a linear range of 3.125–200.0 ng/mL and LOD of 0.234 ng/mL. The detection results of 15 clinical serum real samples demonstrated a 93.7% correlation with the market-accepted ECLIA method. The proposed method take advantages of simplicity and rapid response, offering a novel approach for tumor marker analysis with significant potential. [ABSTRACT FROM AUTHOR] |
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| ISSN: | 20794991 |
| DOI: | 10.3390/nano16080479 |
