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Lateral flow assay for detecting aflatoxin B1 based on prussian blue nanoparticles.

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Title: Lateral flow assay for detecting aflatoxin B1 based on prussian blue nanoparticles.
Authors: Zhong, Ying1 (AUTHOR), Zhang, Hao1 (AUTHOR), Yuan, Yihan1 (AUTHOR), Zou, Bole1 (AUTHOR), Hu, Chao1 (AUTHOR), Tu, Qin1 (AUTHOR) tuqin@nwsuaf.edu.cn, Wang, Jinyi1 (AUTHOR) jywang@nwsuaf.edu.cn
Source: Microchimica Acta. May2026, Vol. 193 Issue 5, p1-12. 12p.
Subjects: Aflatoxins, Prussian blue, Biosensors, Rapid diagnostic tests, Food safety, Point-of-care testing
Abstract: Current detection methods for aflatoxin B1 (AFB1) are often hindered by interference from complex sample matrices and typically require bulky instrumentation and specialized operations, which limit their utility for rapid on-site screening. To overcome these challenges, a lateral flow assay (LFA) strip was developed that employs Prussian blue nanoparticles (PBNP) as a signal label. This approach substitutes conventional gold nanoparticles with cost-effective and easily synthesized PBNP. By utilizing EDC/NHS coupling, PBNP was conjugated to an AFB1-specific aptamer, establishing a competitive detection system. The experiments were optimized for probe coupling efficiency and reaction conditions, and the performance of the test strip was comprehensively evaluated. Results indicated that the constructed PBNP-LFA exhibited reliable detection performance, with a detection limit of 20.92 µg/kg, and excellent specificity for AFB1, with the entire detection process completed within 10 min. Furthermore, in practical food samples, such as corn flour and peanuts, this method demonstrated remarkable resistance to matrix interference and good reliability. This study presents a promising new strategy for rapid, low-cost point-of-care detection of AFB1. Highlights: A novel lateral flow assay has been developed for the rapid detection of aflatoxin B1 (AFB1), utilizing cost-effective and easily synthesized Prussian blue nanoparticles as signal labels. The assay demonstrates reliable detection performance, with a limit of detection of 20.92 µg/kg and a linear detection range of 0.1–50 µM, exhibiting a correlation coefficient of 0.9896 for AFB1. The lateral flow assay demonstrates excellent specificity, repeatability, and stability over a period of 10 days at 4 °C, while exhibiting negligible matrix interference in corn and wheat flour samples. The method simplifies operation, avoids large-scale instrumentation, and is suitable for on-site rapid screening of AFB1 in food and agricultural products. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
Description
Abstract:Current detection methods for aflatoxin B1 (AFB1) are often hindered by interference from complex sample matrices and typically require bulky instrumentation and specialized operations, which limit their utility for rapid on-site screening. To overcome these challenges, a lateral flow assay (LFA) strip was developed that employs Prussian blue nanoparticles (PBNP) as a signal label. This approach substitutes conventional gold nanoparticles with cost-effective and easily synthesized PBNP. By utilizing EDC/NHS coupling, PBNP was conjugated to an AFB1-specific aptamer, establishing a competitive detection system. The experiments were optimized for probe coupling efficiency and reaction conditions, and the performance of the test strip was comprehensively evaluated. Results indicated that the constructed PBNP-LFA exhibited reliable detection performance, with a detection limit of 20.92 µg/kg, and excellent specificity for AFB1, with the entire detection process completed within 10 min. Furthermore, in practical food samples, such as corn flour and peanuts, this method demonstrated remarkable resistance to matrix interference and good reliability. This study presents a promising new strategy for rapid, low-cost point-of-care detection of AFB1. Highlights: A novel lateral flow assay has been developed for the rapid detection of aflatoxin B1 (AFB1), utilizing cost-effective and easily synthesized Prussian blue nanoparticles as signal labels. The assay demonstrates reliable detection performance, with a limit of detection of 20.92 µg/kg and a linear detection range of 0.1–50 µM, exhibiting a correlation coefficient of 0.9896 for AFB1. The lateral flow assay demonstrates excellent specificity, repeatability, and stability over a period of 10 days at 4 °C, while exhibiting negligible matrix interference in corn and wheat flour samples. The method simplifies operation, avoids large-scale instrumentation, and is suitable for on-site rapid screening of AFB1 in food and agricultural products. [ABSTRACT FROM AUTHOR]
ISSN:00263672
DOI:10.1007/s00604-026-08075-7