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Culture clash: challenging the dogma of microbial diversity.

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Bibliographic Details
Title: Culture clash: challenging the dogma of microbial diversity.
Authors: Donachie, Stuart P.1 donachie@hawaii.edu, Foster, Jamie S.2, Brown, Mark V.3
Source: ISME Journal: Multidisciplinary Journal of Microbial Ecology. Jun2007, Vol. 1 Issue 2, p97-99. 3p.
Subjects: Microbial ecology, Environmental sciences, Microbial diversity, Recombinant DNA, Microorganisms
Abstract: The article presents the author's views on reliance on microbial ecology in environmental studies. He says that reliance on microbial ecology perpetuates certain assumptions including, ribosomal RNA gene analyses can be used exclusively for the study of microbial diversity, only a fraction of environmental bacteria 'species' are culturable. He discusses various studies and says that there is a need to determine why so many cultivated microorganisms are not detected in 16S rDNA clone libraries.
Database: Engineering Source
Description
Abstract:The article presents the author's views on reliance on microbial ecology in environmental studies. He says that reliance on microbial ecology perpetuates certain assumptions including, ribosomal RNA gene analyses can be used exclusively for the study of microbial diversity, only a fraction of environmental bacteria 'species' are culturable. He discusses various studies and says that there is a need to determine why so many cultivated microorganisms are not detected in 16S rDNA clone libraries.
ISSN:17517362
DOI:10.1038/ismej.2007.22