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Optimization of chemically defined medium for recombinant Pichia pastoris for biomass production

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Title: Optimization of chemically defined medium for recombinant Pichia pastoris for biomass production
Authors: Ghosalkar, A.1, Sahai, V.1, Srivastava, A.2 asriv2002@yahoo.co.in
Source: Bioresource Technology. Nov2008, Vol. 99 Issue 16, p7906-7910. 5p.
Subjects: Pichia pastoris, Biomass, Trace metals, Biochemical engineering, Fermentation, Industrial microbiology
Abstract: Abstract: A chemically defined medium was optimized for the maximum biomass production of recombinant Pichia pastoris in the fermentor cultures using glycerol as the sole carbon source. Optimization was done using the statistical methods for getting the optimal level of salts, trace metals and vitamins for the growth of recombinant P. pastoris. The response surface methodology was effective in optimizing nutritional requirements using the limited number of experiments. The optimum medium composition was found to be 20g/L glycerol, 7.5g/L (NH4)2SO4, 1g/L MgSO4·7H2O, 8.5g/L KH2PO4, 1.5mL/L vitamin solution and 20mL/L trace metal solution. Using the optimized medium 11.25g DCW/L biomass was produced giving a yield coefficient of 0.55g biomass/g of glycerol in a batch culture. Chemostat cultivation of recombinant P. pastoris was done in the optimized medium at different dilution rates to determine the kinetic parameters for growth on glycerol. Maximum specific growth rate of 0.23h−1 and Monod saturation constant of 0.178g/L were determined by applying Monod model on the steady state data. Products of fermentation pathway, ethanol and acetate, were not detected by HPLC even at higher dilution rates. This supports the notion that P. pastoris cells grow on glycerol by a respiratory route and are therefore an efficient biomass and protein producers. [Copyright &y& Elsevier]
Copyright of Bioresource Technology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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  Data: Optimization of chemically defined medium for recombinant Pichia pastoris for biomass production
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  Data: <searchLink fieldCode="AR" term="%22Ghosalkar%2C+A%2E%22">Ghosalkar, A.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Sahai%2C+V%2E%22">Sahai, V.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Srivastava%2C+A%2E%22">Srivastava, A.</searchLink><relatesTo>2</relatesTo><i> asriv2002@yahoo.co.in</i>
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  Data: <searchLink fieldCode="JN" term="%22Bioresource+Technology%22">Bioresource Technology</searchLink>. Nov2008, Vol. 99 Issue 16, p7906-7910. 5p.
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  Data: <searchLink fieldCode="DE" term="%22Pichia+pastoris%22">Pichia pastoris</searchLink><br /><searchLink fieldCode="DE" term="%22Biomass%22">Biomass</searchLink><br /><searchLink fieldCode="DE" term="%22Trace+metals%22">Trace metals</searchLink><br /><searchLink fieldCode="DE" term="%22Biochemical+engineering%22">Biochemical engineering</searchLink><br /><searchLink fieldCode="DE" term="%22Fermentation%22">Fermentation</searchLink><br /><searchLink fieldCode="DE" term="%22Industrial+microbiology%22">Industrial microbiology</searchLink>
– Name: Abstract
  Label: Abstract
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  Data: Abstract: A chemically defined medium was optimized for the maximum biomass production of recombinant Pichia pastoris in the fermentor cultures using glycerol as the sole carbon source. Optimization was done using the statistical methods for getting the optimal level of salts, trace metals and vitamins for the growth of recombinant P. pastoris. The response surface methodology was effective in optimizing nutritional requirements using the limited number of experiments. The optimum medium composition was found to be 20g/L glycerol, 7.5g/L (NH4)2SO4, 1g/L MgSO4·7H2O, 8.5g/L KH2PO4, 1.5mL/L vitamin solution and 20mL/L trace metal solution. Using the optimized medium 11.25g DCW/L biomass was produced giving a yield coefficient of 0.55g biomass/g of glycerol in a batch culture. Chemostat cultivation of recombinant P. pastoris was done in the optimized medium at different dilution rates to determine the kinetic parameters for growth on glycerol. Maximum specific growth rate of 0.23h−1 and Monod saturation constant of 0.178g/L were determined by applying Monod model on the steady state data. Products of fermentation pathway, ethanol and acetate, were not detected by HPLC even at higher dilution rates. This supports the notion that P. pastoris cells grow on glycerol by a respiratory route and are therefore an efficient biomass and protein producers. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Bioresource Technology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1016/j.biortech.2008.01.059
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        Text: English
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      – SubjectFull: Biomass
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      – SubjectFull: Trace metals
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      – SubjectFull: Biochemical engineering
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      – SubjectFull: Fermentation
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      – SubjectFull: Industrial microbiology
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              M: 11
              Text: Nov2008
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