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Microbial production of hydrogen from starch-manufacturing wastes

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Title: Microbial production of hydrogen from starch-manufacturing wastes
Authors: Yokoi, H. yokoi@cc.miyazaki-u.ac.jp, Maki, R.1, Hirose, J.1, Hayashi, S.1
Source: Biomass & Bioenergy. May2002, Vol. 22 Issue 5, p389. 7p.
Subjects: Industrial microorganisms, Hydrogen production
Abstract: Effective hydrogen production from starch-manufacturing wastes by microorganisms was investigated. Continuous hydrogen production in high yield of 2.7 mol H2 mol−1 glucose was attained by a mixed culture of Clostridium butyricum and Enterobacter aerogenes HO-39 in the starch waste medium consisting of sweet potato starch residue as a carbon source and corn steep liquor as a nitrogen source in a repeated batch culture. Rhodobacter sp. M-19 could produce hydrogen from the supernatant of the culture broth obtained in the repeated batch culture of C. butyricum and E. aerogenes HO-39. Hydrogen yield of 4.5 mol H2 mol−1 glucose was obtained by culturing Rhodobacter sp. M-19 in the supernatant supplemented with 20 μg l−1 Na2MoO4·2H2O and 10 mg l−1 EDTA in a repeated batch culture with pH control at 7.5. Therefore, continuous hydrogen production with total hydrogen yield of 7.2 mol H2 mol−1 glucose from the starch remaining in the starch residue was attained by the repeated batch culture with C. butyricum and E. aerogenes HO-39 and by the successive repeated batch culture with Rhodobacter sp. M-19. [Copyright &y& Elsevier]
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Database: Engineering Source
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Abstract:Effective hydrogen production from starch-manufacturing wastes by microorganisms was investigated. Continuous hydrogen production in high yield of <f>2.7 mol</f> <f>H2 mol−1</f> glucose was attained by a mixed culture of Clostridium butyricum and Enterobacter aerogenes HO-39 in the starch waste medium consisting of sweet potato starch residue as a carbon source and corn steep liquor as a nitrogen source in a repeated batch culture. Rhodobacter sp. M-19 could produce hydrogen from the supernatant of the culture broth obtained in the repeated batch culture of C. butyricum and E. aerogenes HO-39. Hydrogen yield of <f>4.5 mol</f> <f>H2 mol−1</f> glucose was obtained by culturing Rhodobacter sp. M-19 in the supernatant supplemented with <f>20 μg l−1</f> <f>Na2MoO4·2H2O</f> and <f>10 mg l−1</f> EDTA in a repeated batch culture with pH control at 7.5. Therefore, continuous hydrogen production with total hydrogen yield of <f>7.2 mol</f> <f>H2 mol−1</f> glucose from the starch remaining in the starch residue was attained by the repeated batch culture with C. butyricum and E. aerogenes HO-39 and by the successive repeated batch culture with Rhodobacter sp. M-19. [Copyright &y& Elsevier]
ISSN:09619534
DOI:10.1016/S0961-9534(02)00014-4