Bibliographic Details
| Title: |
Physical estimation of triplet phases from two new proteins. |
| Authors: |
Mo, F., Mathiesen, R.H., Alzari, P.M., Lescar, J., Rasmussen, B. |
| Source: |
Acta Crystallographica: Section D (Wiley-Blackwell). Oct2002 Part 2, Vol. 58 Issue 10p2, p1780. 7p. |
| Subjects: |
Crystals, Glycosidases, Triplet state (Quantum mechanics) |
| Abstract: |
Three-beam interference experiments have been performed with crystals of two glycosidases: guinea-fowl hexagonal lysozyme, MW 14.3 kDa, and C. thermocellum endoglucanase Ce1A, MW 40 kDa. In both cases triplet phases could be estimated. Experimental parameters and details of the procedure are presented along with some examples of the results. The average differences between the estimated phases and those calculated from the crystallographic refinements were 17.9 and 15.9°, respectively. A brief discussion of alternative methods for physical phase acquisition is given, including possible strategies for the measurement and application of experimental phases in macromolecular crystallography. [ABSTRACT FROM AUTHOR] |
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| Database: |
Engineering Source |
