Bibliographic Details
| Title: |
Air-sparged microfiltration of enzyme/yeast mixtures: determination of optimal conditions for enzyme recovery |
| Authors: |
Mercier-Bonin, Muriel1, Fonade, Christian2 |
| Source: |
Desalination. 2002, Vol. 148 Issue 1-3, p171. 6p. |
| Subjects: |
Two-phase flow, Fouling, Ultrafiltration |
| Abstract: |
The optimal conditions for the recovery of an enzyme were determined using gas/liquid two-phase flows. When filtering the enzyme-only solution under single-phase flow conditions, a severe fouling occurred. This fouling was manifested as a decline in enzyme mass flux due to a decrease in both flux and transmission. When yeast cells were added under the same experimental conditions, the enzyme mass flux was nearly twofold higher. During gas-sparged microfiltration of the enzyme/yeast mixture in a permeate-recycling mode at the same liquid flow rate, gas/liquid slug flow strongly decreased the mass flux of the enzyme due to the drastic reduction in transmission (70% decrease) even though the permeate flux was improved (140% improvement). However, with a bubble flow pattern, the enzyme mass flux was 25% higher (flux slightly enhanced and high transmission), and during the diafiltration experiment a 13% higher enzyme recovery was achieved. [Copyright &y& Elsevier] |
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| Database: |
Engineering Source |
