ABCC11 Earwax Trait and Genotype Are Suitable Tools for Introductory Labs to Learn Genetics and Molecular Techniques
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| Title: | ABCC11 Earwax Trait and Genotype Are Suitable Tools for Introductory Labs to Learn Genetics and Molecular Techniques |
|---|---|
| Language: | English |
| Authors: | Tohru Ohta, Rie Takai, Akiko Yoshida, Durga Paudel, Sarita Giri, Takao Kitagawa, Toshiya Arakawa, Yasuhiro Kuramitsu, Tomoharu Tokutomi (ORCID |
| Source: | Biochemistry and Molecular Biology Education. 2025 53(6):603-609. |
| Availability: | Wiley. Available from: John Wiley & Sons, Inc. 111 River Street, Hoboken, NJ 07030. Tel: 800-835-6770; e-mail: cs-journals@wiley.com; Web site: https://www.wiley.com/en-us |
| Peer Reviewed: | Y |
| Page Count: | 7 |
| Publication Date: | 2025 |
| Document Type: | Journal Articles Reports - Research |
| Education Level: | Secondary Education High Schools |
| Descriptors: | Genetics, Science Experiments, Secondary School Science, Computer Assisted Instruction, Molecular Biology, High School Students, Laboratory Experiments, Science Instruction |
| DOI: | 10.1002/bmb.70010 |
| ISSN: | 1470-8175 1539-3429 |
| Abstract: | Professional experiments in genetic research usually start in a class at university. However, interest in genetic research techniques from an early age is essential. We have continuously performed a short genetic experimental course for high school students using a simple molecular experiment and computer-based learning for Mendelian inheritance. We utilized the phenotype-genotype association of ABCC11 polymorphism (rs17822931), in which the A allele at rs17822931 of ABCC11 is the recessive genotype for the dry earwax phenotype. Conversely, the G allele is the dominant genotype for the wet earwax phenotype. The phenotype is primarily determined by a single-nucleotide polymorphism (SNP), rs17822931, with the A or G allele, which has global prevalence with few exceptions. The A or G allele is easily typed using the DdeI restriction enzyme. The students experienced molecular techniques and created computer-equipped pedigree charts using the software "f-tree." The earwax trait is an excellent tool for genetic education to understand Mendelian inheritance, genotype-phenotype association, PCR cloning, and restriction enzyme digestion, and it is suitable for discussing the historical and geographical migration of the ancient Mongolian people. After the short course, a survey showed that 81% of students were satisfied, including 71% of very satisfied students. |
| Abstractor: | As Provided |
| Entry Date: | 2025 |
| Accession Number: | EJ1490578 |
| Database: | ERIC |
| Abstract: | Professional experiments in genetic research usually start in a class at university. However, interest in genetic research techniques from an early age is essential. We have continuously performed a short genetic experimental course for high school students using a simple molecular experiment and computer-based learning for Mendelian inheritance. We utilized the phenotype-genotype association of ABCC11 polymorphism (rs17822931), in which the A allele at rs17822931 of ABCC11 is the recessive genotype for the dry earwax phenotype. Conversely, the G allele is the dominant genotype for the wet earwax phenotype. The phenotype is primarily determined by a single-nucleotide polymorphism (SNP), rs17822931, with the A or G allele, which has global prevalence with few exceptions. The A or G allele is easily typed using the DdeI restriction enzyme. The students experienced molecular techniques and created computer-equipped pedigree charts using the software "f-tree." The earwax trait is an excellent tool for genetic education to understand Mendelian inheritance, genotype-phenotype association, PCR cloning, and restriction enzyme digestion, and it is suitable for discussing the historical and geographical migration of the ancient Mongolian people. After the short course, a survey showed that 81% of students were satisfied, including 71% of very satisfied students. |
|---|---|
| ISSN: | 1470-8175 1539-3429 |
| DOI: | 10.1002/bmb.70010 |
