Comparison of qNMR Spectroscopy and an Enzyme-Based Standard Method for the Determination of Ethanol in Nonalcoholic Beer
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| Title: | Comparison of qNMR Spectroscopy and an Enzyme-Based Standard Method for the Determination of Ethanol in Nonalcoholic Beer |
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| Language: | English |
| Authors: | JessicaL. Bluitt, Elisa M. Woolridge, Neil Fitzgerald (ORCID |
| Source: | Journal of Chemical Education. 2025 102(9):4109-4114. |
| Availability: | Division of Chemical Education, Inc. and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc |
| Peer Reviewed: | Y |
| Page Count: | 6 |
| Publication Date: | 2025 |
| Document Type: | Journal Articles Reports - Descriptive |
| Education Level: | Higher Education Postsecondary Education |
| Descriptors: | Science Instruction, Chemistry, Spectroscopy, College Science, Undergraduate Students, Science Experiments |
| DOI: | 10.1021/acs.jchemed.5c00783 |
| ISSN: | 0021-9584 1938-1328 |
| Abstract: | The concept of using appropriate statistical methods to compare results from a newly proposed analytical method to a standard method can be poorly understood among undergraduate students. We developed a learning activity for an undergraduate analytical chemistry course to provide a practical demonstration of method comparison. The activity consists of students collecting data on the ethanol content of nonalcoholic beers via a quantitative NMR spectroscopic method, using a benchtop NMR, and an enzyme-based UV spectrophotometric method using a commercially available enzyme assay kit. Students are then able to apply a simple statistical test to determine whether the two methods are equivalent. The activity was successfully incorporated into a course. Students were able to measure ethanol concentrations using both methods and compare data using a Student's t-test. Survey results indicate that the students gained a better understanding of the use of statistics in evaluating an analytical method. |
| Abstractor: | As Provided |
| Entry Date: | 2026 |
| Accession Number: | EJ1491077 |
| Database: | ERIC |
| Abstract: | The concept of using appropriate statistical methods to compare results from a newly proposed analytical method to a standard method can be poorly understood among undergraduate students. We developed a learning activity for an undergraduate analytical chemistry course to provide a practical demonstration of method comparison. The activity consists of students collecting data on the ethanol content of nonalcoholic beers via a quantitative NMR spectroscopic method, using a benchtop NMR, and an enzyme-based UV spectrophotometric method using a commercially available enzyme assay kit. Students are then able to apply a simple statistical test to determine whether the two methods are equivalent. The activity was successfully incorporated into a course. Students were able to measure ethanol concentrations using both methods and compare data using a Student's t-test. Survey results indicate that the students gained a better understanding of the use of statistics in evaluating an analytical method. |
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| ISSN: | 0021-9584 1938-1328 |
| DOI: | 10.1021/acs.jchemed.5c00783 |
